The nucleotide sequence of the Bali-PstI fragment of T5 DNA, 1347 bp in length, coding for S-exonuclease (D 15 gene), has heen determined. A coding region of the gene contains 873 bp and is preceded by a typical Shine-Dalgarno sequence. The D15 gene belongs to a cluster, consisting of at least 3 genes, in which a termination codon of a preceding gene overlaps an initiation codon of the following one. The sequence contains an open reading frame for 291 amino acid residues. The molecular mass of the S-exonuclease calculated from the predicted amino acid sequence is 33400 Da.Bacteriophage T5 DNA sequencing D15 gene ISI element
Bacteriophage T5 BglII/HindIII DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322. The analysis of DNA sequence indicates that tRNA genes code isoacceptor tRNAsSer (tRNASer
1 and tRNASer
2) with anticodons UGA and GGA, respectively. The main unusual structural feature of these tRNAs is the presence of extra non‐basepaired nucleotides in the joinings of stem ‘b’ with stems ‘a’ and ‘c’.
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