The novel SNPs of leptin gene and their associations with growth traits in Chinese Nanjiang Yellow goat

Wang, Cen; Zhang, Hao; Niu, Lili; Guo, Jiazhong; Jia, Xianbo; Wang, Linjie; Li, Li; Zhang, Hongping; Zhong, Tao

doi:10.1016/j.gene.2015.06.073

Cited by 13 publications

(17 citation statements)

References 35 publications

(34 reference statements)

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“…The 5 bp indel was in HWE ( P > 0.05); however, the 16 bp locus was not ( P < 0.05), because of the lower number of observed DD genotypes. One possible reason for this is rapid, powerful, and effective selection, which could affect the allelic balance of the indel locus (Zhao et al, 2013 ; Wang et al, 2015 ). Therefore, our data indicate that the selection pressure was more powerful on 16 bp than the 5 bp indel locus in the investigated goat population.…”

Section: Discussionmentioning

confidence: 99%

Insertion/Deletion Within the KDM6A Gene Is Significantly Associated With Litter Size in Goat

et al. 2018

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A previous whole-genome association analysis identified lysine demethylase 6A (KDM6A), which encodes a type of histone demethylase, as a candidate gene associated to goat fecundity. KDM6A gene knockout mouse disrupts gametophyte development, suggesting that it has a critical role in reproduction. In this study, goat KDM6A mRNA expression profiles were determined, insertion/deletion (indel) variants in the gene identified, indel variants effect on KDM6A gene expression assessed, and their association with first-born litter size analyzed in 2326 healthy female Shaanbei white cashmere goats. KDM6A mRNA was expressed in all tissues tested (heart, liver, spleen, lung, kidney, muscle, brain, skin and testis); the expression levels in testes at different developmental stages [1-week-old (wk), 2, 3 wk, 1-month-old (mo), 1.5 and 2 mo] indicated a potential association with the mitosis-to-meiosis transition, implying that KDM6A may have an essential role in goat fertility. Meanwhile, two novel intronic indels of 16 bp and 5 bp were identified. Statistical analysis revealed that only the 16 bp indel was associated with first-born litter size (P < 0.01), and the average first-born litter size of individuals with an insertion/insertion genotype higher than that of those with the deletion/deletion genotype (P < 0.05). There was also a significant difference in genotype distributions of the 16 bp indel between mothers of single-lamb and multi-lamb litters in the studied goat population (P = 0.001). Consistently, the 16 bp indel also had a significant effect on KDM6A gene expression. Additionally, there was no significant linkage disequilibrium (LD) between these two indel loci, consistent with the association analysis results. Together, these findings suggest that the 16 bp indel in KDM6A may be useful for marker-assisted selection (MAS) of goats.

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Section: Discussionmentioning

confidence: 99%

Insertion/Deletion Within the KDM6A Gene Is Significantly Associated With Litter Size in Goat

et al. 2018

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“…Significant associations between growth traits and polymorphisms in LEPT have also been reported in goats (Wang et al, 2015), whereas other studies have demonstrated the associations of LEPT mutations with the carcass and meat quality traits in cattle (Tian et al, 2013). These associations are possibly explained by the role that leptin plays in the energy metabolic regulation through its action on the hypothalamic-pituitary-adrenal axis, with subsequent effects on the growth process of different species (Delavaud et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…In bovine adipose tissue, growth hormone regulates the gene expression of leptin (Houseknecht et al, 2000), a hormone that functions as a lipostatic signal to regulate body weight (BW), feed intake, expenditure energy, reproduction, and functions of the immune system (Houseknecht et al, 1998;Larsson et al, 1998;Lord et al, 1998;Woods et al, 1998;Cunningham et al, 1999;Delavaud et al, 2002;Garcia et al, 2002;Nkrumah et al, 2004). Genomic variations in leptin (LEPT) gene have been associated with growth traits in goats (Wang et al, 2015) and beef cattle (Tian et al, 2013). The phenomenon of excessive muscle fiber formation in Belgian Blue and Piedmontese cattle, known as "doublemuscling," has been associated with myostatin (MSTN) gene (Rehfeldt et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Identification of SNPs in growth-related genes in Colombian creole cattle

et al. 2016

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ABSTRACT. Colombian creole cattle have important adaptation traits related to heat tolerance and reproductive and productive efficiency. Romosinuano (ROMO) and Blanco Orejinegro (BON) are the most common breeds used by Colombian cattle breeders. Growth traits are of prime importance in these animals, which are mainly raised for beef production. Genes encoding growth hormone, growth hormone receptor, homeobox protein, insulin growth factor binding protein 3, leptin, and myostatin have been associated with physiological growth pathways in cattle and other species. We therefore aimed to identify single nucleotide polymorphisms (SNPs) within these genes in ROMO, BON, and Zebu cattle. DNA regions of these genes were sequenced in 386 animals; 47 new SNPs were found, of which 14 were located in the exonic regions, thereby changing the protein sequence. An association of SNPs with weaning weight (WW), daily weight gain at weaning (DWG), and weight at 16 months (W16M) traits was deduced. The genetic analysis revealed several SNPs related to these traits. The SNP GhRE06.2 had a significant association with WW and the SNP Lep03.4 was highly associated with DWG and W16M. Other polymorphisms were significantly associated with WW and DWG, although they did not surpass the Bonferroni significance threshold. The new mutations identified may indicate important points of genetic control in the DNA that could be responsible for changes in the expression of the analyzed traits. These SNPs might be used in future breeding programs to improve the productive performance of cattle in beef farms.

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“…In this context, the relevant gene should be studied in Honamli and Hair goats by detecting polymorphism using sequence analysis. WANG et al, (2015) have found six novel mutations in LEP gene, which have been associated with growth traits in six native Chinese goat breeds. On the other hand, some SNPs have been found to be associated growth traits in cattle (YANG et al, 2007;SILVA et al, 2014).…”

Section: Yijklmno = μ + Ai + Bj + Ck + DL + Fm + Gn + Eijklmnomentioning

confidence: 99%

Identifying polymorphism in some genes and their effects on growth performance in Honamli and hair goat breeds

Korkmaz-Ağaoğlu¹,

Elmaz²,

Akyüz³

et al. 2019

Genetika

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2019): Identifying polymorphism in some genes and their effects in growth performance in Honamli and Hair goat breeds.-Genetika, Vol 51, No.3, 995-1008. The aim of this study was to detect growth hormone (GH), leptin (LEP), pituitary transcription factor-1 (POU1F1), bone morphogenetic protein-15 (BMP15) and myostatin (MSTN) genes polymorphisms, to investigate the genetic structure and allele frequencies value for these polymorphisms and to determine their influence on some growth traits (live weight and linear body measurements as growth traits on 90, 120, 180 and 365 days) in Honamli and Hair goat breeds. For these purposes, a total of 300 goats were used, genotyping was performed on 150 Honamli and 150 Hair goat breeds. Genotypes were identified by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Phenotypic data of growth traits of each individual goat were collected. A 422 bp product and a 116 bp product of GH1 and GH2 respectively, a 450 bp product of POU1F1, a 152 bp and 400 bp product of LEP EX2 and LEP INT2 respectively, a 497 bp product of MSTN and a 141 bp product of BMP15, thus 7 loci of 5 genes, were amplified 996 GENETIKA, Vol. 51, No3, 995-1008, 2019 by PCR. The effect of genotypes at each locus on growth traits was assessed with general linear models. Birth weights, live weights and linear body measurements in the Honamli goat were higher in Hair goat. Investigated breeds were in Hardy-Weinberg equilibrium for GH2-HaeIII, POU1F1-Pst1 and MSTN-DraI polymorphisms, but not for the GH1-HaeIII polymorphism (p<0.001). Whereas, LEP and BMP15 genes were monomorphic in both breeds. On the other hand, no significant associations were found between examined growth traits and any of the studied polymorphisms in the Honamli and Hair goat breeds. This study reported the existence of genetic polymorphisms in the GH, POU1F1 and MSTN genes and their effects on growth traits in Honamli and Hair goat breeds were investigated for the first time. The present results led us to conclude that although the GH2-HaeIII, POU1F1-Pst1 and MSTN-DraI polymorphism presented adequate segregation for association studies in Honamli and Hair goat breeds, it does not seem to be a promising selection tool for growth traits in Honamli and Hair goat breeds.

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The novel SNPs of leptin gene and their associations with growth traits in Chinese Nanjiang Yellow goat

Cited by 13 publications

References 35 publications

Insertion/Deletion Within the KDM6A Gene Is Significantly Associated With Litter Size in Goat

Insertion/Deletion Within the KDM6A Gene Is Significantly Associated With Litter Size in Goat

Identification of SNPs in growth-related genes in Colombian creole cattle

Identifying polymorphism in some genes and their effects on growth performance in Honamli and hair goat breeds

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