Identification of SNPs in growth-related genes in Colombian creole cattle

Abstract

ABSTRACT. Colombian creole cattle have important adaptation traits related to heat tolerance and reproductive and productive efficiency. Romosinuano (ROMO) and Blanco Orejinegro (BON) are the most common breeds used by Colombian cattle breeders. Growth traits are of prime importance in these animals, which are mainly raised for beef production. Genes encoding growth hormone, growth hormone receptor, homeobox protein, insulin growth factor binding protein 3, leptin, and myostatin have been associated with physiological growth pathways in cattle and other species. We therefore aimed to identify single nucleotide polymorphisms (SNPs) within these genes in ROMO, BON, and Zebu cattle. DNA regions of these genes were sequenced in 386 animals; 47 new SNPs were found, of which 14 were located in the exonic regions, thereby changing the protein sequence. An association of SNPs with weaning weight (WW), daily weight gain at weaning (DWG), and weight at 16 months (W16M) traits was deduced. The genetic analysis revealed several SNPs related to these traits. The SNP GhRE06.2 had a significant association with WW and the SNP Lep03.4 was highly associated with DWG and W16M. Other polymorphisms were significantly associated with WW and DWG, although they did not surpass the Bonferroni significance threshold. The new mutations identified may indicate important points of genetic control in the DNA that could be responsible for changes in the expression of the analyzed traits. These SNPs might be used in future breeding programs to improve the productive performance of cattle in beef farms.

Optimization of high molecular weight DNA extraction methods in shrimp for a long-read sequencing platform

“…Genome sequence has enabled the identification of single nucleotide polymorphisms (SNPs) and genetic markers for desirable traits (Zwane et al, 2019) such as productivity, disease resistance and reproductive maturation (Guppy et al, 2018). For instance, specific SNPs for growth performance and immunity traits have been identified and utilized for breeding programs assisted by genomic selection in Blanco Orejinegro cattle (Martínez et al, 2010;Martínez et al, 2016), chicken (Li et al, 2013;Xie et al, 2012) and pig (Geraci et al, 2019;Yang et al, 2019). Genomics has indeed contributed to the exponential growth of terrestrial animal production, yet it has not been fully exploited in aquaculture industry due to several challenges in obtaining whole genome sequences of marine organisms.…”

Asociación de polimorfismos del gen Leptina con calidad seminal en toros raza Carora

“…Polimorfismos del gen LEP han sido asociados con la concentración de Leptina circulante [30] y con rasgos de importancia económica en diferentes especies, tales como, de producción de leche [54], características de la canal en ganado Nellore [63], calidad de la carne en bovinos y cerdos [10,66], rasgos de crecimiento en cabras y novillos [44,69], eficiencia alimenticia en toros Nellore [48] y rasgos de fertilidad [29]. En el caso del rendimiento reproductivo del macho, se ha comprobado el efecto de esta hormona sobre la pubertad, espermatogénesis, maduración, y motilidad espermática en diferentes especies [33,45] y sobre la capacitación espermática en cerdos y búfalos (Bubalus bubalis) [6,31].…”

Polymorphism of Bovine Growth Hormone Receptor Gene (g.3338A>G) and Its Association with Body Measurements and Body Weight in Pasundan Cows

“…Previous studies reported that polymorphism of bGHR gene was occured in Bos javanicus breed (Maskur et al, 2014;Zulkharnaim et al, 2010), Bos taurus breeds (Di Stasio et al, 2005;Olenski et al, 2010;Misrianti et al, 2011;Akad et al, 2012) and Bos indicus breeds (Zulkharnaim et al, 2010;Deepika & Salar, 2013). One of the polymorphism in bGHR gene were detected in exon 10 (Hadi et al, 2015;Martinez et al, 2016). The single nucleotide polymorphism (SNP) in the exon 10 of bGHR gene was detected in position g.3338A>G (transition mutation) and affecting the amino acid change from Serine to Glycine (Deepika & Salar, 2013;Martinez et al, 2016).…”

“…One of the polymorphism in bGHR gene were detected in exon 10 (Hadi et al, 2015;Martinez et al, 2016). The single nucleotide polymorphism (SNP) in the exon 10 of bGHR gene was detected in position g.3338A>G (transition mutation) and affecting the amino acid change from Serine to Glycine (Deepika & Salar, 2013;Martinez et al, 2016). The previous study reported that this SNP also occurred in some Indonesian native cattle such as Bali, Pesisir (Zulkharnaim et al, 2010) and Friesian Holstein (Misrianti et al, 2011).…”