The NAD + -dependent deacetylase, Bifidobacterium longum Sir2 in response to oxidative stress by deacetylating SigH (σ H ) and FOXO3a in Bifidobacterium longum and HEK293T cell respectively

Guo, Qing; Li, Shiyu; Xie, Yu; Zhang, Qian; Liu, Mengge; Xu, Zhenrui; Sun, Han; Yang, Yan

doi:10.1016/j.freeradbiomed.2017.05.012

Cited by 27 publications

(27 citation statements)

References 41 publications

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“…A protein related with transcription‐σ H , as the effect target of sir2 of B . longum in prokaryotic cells (Guo et al, ). To identify the substrate of EF‐Sir2 protein, on the basis of the report, we first analysed related transcription factors in 91 lysine‐acetylated proteins, including rpoA, sigA, rho, D920_01001, D920_02736, and D920_00630.…”

Section: Resultsmentioning

confidence: 99%

“…Interestingly, differ from our previous results of anaerobic bacteria sir2‐like gene, which has shown that deacetylation of σ H by means of BL‐sir2 accounts for antioxidant reponse in B . longum (Guo et al, ). Although both Rho and σ H related to transcription factor, the former regulated glucose metabolism related enzymes of E .…”

Section: Discussionmentioning

confidence: 99%

“…As was done for the previous methods (Guo et al, ), the total cellular protein of E . faecalis were collected and incubated.…”

Section: Methodsmentioning

confidence: 99%

“…CobB also been shown to be involved in transcriptional regulation of the stress responsive promoter cpxP (Lima et al, ), which suggested that the bacterial sir2 has a precise molecular target for energy metabolism. Our study of the sir2 homologues of an anaerobic probiotics Bifidobacterium longum showed that it was associated with the oxidative stress response transcription factor δ H (Guo et al, ). Moreover, the regulation of partial metabolic enzymes by Sir2 protein (Wang et al, ; Yu, Kim, Moon, Ryu, & Pan, ) also showed that the prokaryotic Sir2 protein is functionally similar to the mammalian sirtuin protein family, especially the metabolic process.…”

Section: Introductionmentioning

confidence: 99%

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Enterococcus faecalis sir2-like gene enhances aerobic metabolism of themselves and mitochondrial respiration of mammal cells to bring about improving metabolic syndrome through the PGC-1α pathway

Fei

et al. 2019

J Tissue Eng Regen Med

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Recent studies showed that probiotics could improve metabolic syndrome, making the identification of factors affecting metabolic control more important than ever.The mammalian sirtuin protein family has received much attention for its regulatory role, especially in various mitochondrial ATP, glucose, and lipid metabolic pathways.However, compared with the mammalian sirtuin protein family, the function of prokaryotic sir2 protein is much less known. We studied the effects of probiotics sir2 protein on cell energy metabolize pathway, which showed that deletion of Enterococcus faecalis sir2 inhibited the aerobic oxidation of bacteria and increased the bacterial fermentation. The study of EF-sir2 (sir2 protein of E. faecalis) role of molecular targets demonstrated that deacetylation of EF-sir2 was via Rho upregulating in E. faecalis.When transfected into HEK293T cells, EF-sir2 could significantly facilitate aerobic oxidation of glucose, enhance the respiration to generate more ATP, and cause upregulation of NRF1 target gene. Then, we found EF-sir2 could increase activity of PGC-1α by deacetylation and PGC-1α inhibition decreased the expression of NRF1 target gene. Finally, we demonstrated that EF-sir2 could significantly improve the metabolic index of mammalian cells through insulin resistanced model in vitro and metabolic syndrome rat model in vivo. Our results first revealed that prokaryotic sir2 genes affect the molecular mechanism of cellular metabolism and the regulatory of cell homeostasis in prokaryotic and mammalian cells, suggesting that EF-sir2 has a positive regulatory effect on metabolic disturbance and may be used for the prevention and treatment of pathological processes related to metabolic syndrome. KEYWORDS cellular energy, metabolic syndrome, NRF1, PGC-1α, probiotics, sir2-like gene * Shiyu Li and Zhengbin Fei contributed equally to this work.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…As was done for the previous methods (Guo et al, ), the total cellular protein of E . faecalis were collected and incubated.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enterococcus faecalis sir2-like gene enhances aerobic metabolism of themselves and mitochondrial respiration of mammal cells to bring about improving metabolic syndrome through the PGC-1α pathway

Fei

et al. 2019

J Tissue Eng Regen Med

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“…For example, oxygen reacts with metals present in heme‐dependent cytochrome oxidase, which uses O 2 as substrate (Cesselin et al., 2011). In addition, reaction of O 2 with certain metals forms products, such as superoxide anion radicals (O 2 − ), hydrogen peroxide (H 2 O 2 ), and hydroxyl radicals (OH − ), which are more harmful to cells than O 2 (Guo et al., 2017).…”

Section: Environmental Stressesmentioning

confidence: 99%

Updates on understanding of probiotic lactic acid bacteria responses to environmental stresses and highlights on proteomic analyses

Mbye

Baig

AbuQamar

et al. 2020

Comp Rev Food Sci Food Safe

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Probiotics are defined as live microorganisms that improve the health of the host when administered in adequate quantities. Nonetheless, probiotics encounter extreme environmental conditions during food processing or along the gastrointestinal tract. This review discusses different environmental stresses that affect probiotics during food preparation, storage, and along the alimentary canal, including high temperature, low temperature, low and alkaline pH, oxidative stress, high hydrostatic pressure, osmotic pressure, and starvation. The understanding of how probiotics deal with environmental stress and thrive provides useful information to guide the selection of the strains with enhanced performance in specific situations, in food processing or during gastrointestinal transit. In most cases, multiple biological functions are affected upon exposure of the cell to environmental stress. Sensing of sublethal environmental stress can allow for adaptation processes to occur, which can include alterations in the expression of specific proteins.

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Genome of Bifidobacterium longum NCIM 5672 provides insights into its acid-tolerance mechanism and probiotic properties

et al. 2021

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The NAD + -dependent deacetylase, Bifidobacterium longum Sir2 in response to oxidative stress by deacetylating SigH (σ H ) and FOXO3a in Bifidobacterium longum and HEK293T cell respectively

Cited by 27 publications

References 41 publications

Enterococcus faecalis sir2-like gene enhances aerobic metabolism of themselves and mitochondrial respiration of mammal cells to bring about improving metabolic syndrome through the PGC-1α pathway

Enterococcus faecalis sir2-like gene enhances aerobic metabolism of themselves and mitochondrial respiration of mammal cells to bring about improving metabolic syndrome through the PGC-1α pathway

Updates on understanding of probiotic lactic acid bacteria responses to environmental stresses and highlights on proteomic analyses

Genome of Bifidobacterium longum NCIM 5672 provides insights into its acid-tolerance mechanism and probiotic properties

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