The myosin motor domain-containing chitin synthase PdChsVII is required for development, cell wall integrity and virulence in the citrus postharvest pathogen Penicillium digitatum

Gandía, Mónica; Harries, Eleonora; Marcos, José F.

doi:10.1016/j.fgb.2014.04.002

Cited by 41 publications

(68 citation statements)

References 76 publications

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“…Therefore, further characterization of the evolution of the fungal CHSs could not be achieved based on phylogenetic and domain structure analyses. Unexpectedly, the 7 clades corresponded exactly to the 7 classes of CHSs that were previously reported based on a small number of fungal CHSs510111213. Consequently, we used the previously proposed nomenclature to name the 7 clades.…”

Section: Resultsmentioning

confidence: 76%

“…False positives were removed by examining the presence of conserved domains and motifs of CHSs, resulting in the identification of 1,571CHSs (Fig. S1, Table S2) that included all CHSs identified in several previous studies6111213, suggesting that our method for retrieving CHSs from fungal genomes was effective.…”

Section: Resultsmentioning

confidence: 99%

“…Chs genes are involved in pathogenicity in mammal pathogenic fungi and many plant pathogenic fungi121318253334, but their functions in pathogenicity have not been studied in insect pathogenic fungi. In the present study, we investigated the functions of Chs genes in the model insect pathogenic fungus M. robertsii .…”

Section: Resultsmentioning

confidence: 99%

“…Using the conserved region CON1, a number of studies have investigated the evolution of fungal CHSs. When a small number of CHSs from dikarya fungi were used, fungal CHSs could be phylogenetically divided into 7 classes (I to VII) with high bootstrap support510111213. The 7 classes of CHS are usually grouped into 3 divisions, each with distinct domain structures11.…”

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confidence: 99%

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Evolution of the chitin synthase gene family correlates with fungal morphogenesis and adaption to ecological niches

Liu

Zhang

et al. 2017

Sci Rep

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The fungal kingdom potentially has the most complex chitin synthase (CHS) gene family, but evolution of the fungal CHS gene family and its diversification to fulfill multiple functions remain to be elucidated. Here, we identified the full complement of CHSs from 231 fungal species. Using the largest dataset to date, we characterized the evolution of the fungal CHS gene family using phylogenetic and domain structure analysis. Gene duplication, domain recombination and accretion are major mechanisms underlying the diversification of the fungal CHS gene family, producing at least 7 CHS classes. Contraction of the CHS gene family is morphology-specific, with significant loss in unicellular fungi, whereas family expansion is lineage-specific with obvious expansion in early-diverging fungi. ClassV and ClassVII CHSs with the same domain structure were produced by the recruitment of domains PF00063 and PF08766 and subsequent duplications. Comparative analysis of their functions in multiple fungal species shows that the emergence of ClassV and ClassVII CHSs is important for the morphogenesis of filamentous fungi, development of pathogenicity in pathogenic fungi, and heat stress tolerance in Pezizomycotina fungi. This work reveals the evolution of the fungal CHS gene family, and its correlation with fungal morphogenesis and adaptation to ecological niches.

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Section: Resultsmentioning

confidence: 76%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Evolution of the chitin synthase gene family correlates with fungal morphogenesis and adaption to ecological niches

Liu

Zhang

et al. 2017

Sci Rep

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“…After 10 days, the produced conidia were collected, filtered with 5 μm pore filters and counted in a Neubauer improved chamber (Marienfeld, Germany). The yield of conidia was normalised to the surface of the fungal colony according to Gandía et al [26]. For each experimental treatment (growth and conidiation rates), five Petri dishes for each Trichoderma spp.…”

Section: Methodsmentioning

confidence: 99%

Integrated control of wood destroying basidiomycetes combining Cu-based wood preservatives and Trichoderma spp.

et al. 2017

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The production of new generation of wood preservatives (without addition of a co-biocide) in combination with an exchange of wood poles on identical sites with high fungal inoculum, has resulted in an increase of premature failures of wood utility poles in the last decades. Wood destroying basidiomycetes inhabiting sites where poles have been installed, have developed resistance against wood preservatives. The objective of the in vitro studies was to identify a Trichoderma spp. with a highly antagonistic potential against wood destroying basidiomycetes that is capable of colonizing Cu-rich environments. For this purpose, the activity of five Trichoderma spp. on Cu-rich medium was evaluated according to its growth and sporulation rates. The influence of the selected Trichoderma spp. on wood colonization and degradation by five wood destroying basidiomycetes was quantitatively analyzed by means of dry weight loss of wood specimens. Furthermore, the preventative effect of the selected Trichoderma spp. in combination with four Cu-based preservatives was also examined by mass loss and histological changes in the wood specimens. Trichoderma harzianum (T-720) was considered the biocontrol agent with higher antagonistic potential to colonize Cu-rich environments (up to 0.1% CuSO4 amended medium). T. harzianum demonstrated significant preventative effect on wood specimens against four wood destroying basidiomycetes. The combined effect of T. harzianum and Cu-based wood preservatives demonstrated that after 9 months incubation with two wood destroying basidiomycetes, wood specimens treated with 3.8 kg m-3 copper-chromium had weight losses between 55–65%, whereas containers previously treated with T. harzianum had significantly lower weight losses (0–25%). Histological studies on one of the wood destroying basidiomycetes revealed typical decomposition of wood cells by brown-rot fungi in Cu-impregnated samples, that were notably absent in wood specimens previously exposed to T. harzianum. It is concluded that carefully selected Trichoderma isolates can be used for integrated wood protection against a range of wood destroying basidiomycetes and may have potential for integrated wood protection in the field.

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9 Chitin Synthesis and Fungal Cell Morphogenesis

Roncero

Sánchez‐Díaz

Valdivieso

2016

Biochemistry and Molecular Biology

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The myosin motor domain-containing chitin synthase PdChsVII is required for development, cell wall integrity and virulence in the citrus postharvest pathogen Penicillium digitatum

Cited by 41 publications

References 76 publications

Evolution of the chitin synthase gene family correlates with fungal morphogenesis and adaption to ecological niches

Evolution of the chitin synthase gene family correlates with fungal morphogenesis and adaption to ecological niches

Integrated control of wood destroying basidiomycetes combining Cu-based wood preservatives and Trichoderma spp.

9 Chitin Synthesis and Fungal Cell Morphogenesis

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