The morphological normalcy of the sperm nucleus and pregnancy rate of intracytoplasmic injection with morphologically selected sperm

Berkovitz, Arie; Eltes, F.; Yaari, Shlomit; Katz, Nathan; Barr, Ian G.; Fishman, Ami; Bartoov, B.

doi:10.1093/humrep/deh545

Cited by 273 publications

(206 citation statements)

References 16 publications

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“…Traditionally, semen parameters like sperm count, motility and morphology were considered for assessment of male factor. Of these, sperm morphology is much emphasized recently and is proven to be one of the important predictors in determining the fertilizing capacity of sperm both in vivo and in vitro conditions [23][24][25]. Several clinical studies have demonstrated a clear association between sperm head morphology and improvement in IVF success rate [4,26,27].…”

Section: Discussionmentioning

confidence: 99%

CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation

Sivanarayana

Krishna

Prakash³

et al. 2012

J Assist Reprod Genet

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Purpose The present study was undertaken to evaluate the effects of morphokinetic abnormalities of human spermatozoa on chromatin packing and DNA integrity and possible beneficial effects of sperm selection in ICSI. Methods Semen samples from 1002 patients were analysed for morphology and motility using CASA. Protamine status and DNA fragmentation were analysed by chromomycin A3 staining and sperm chromatin dispersion assay respectively. Results Sperms with elongated, thin, round, pyri, amorphous, micro and macro forms were significantly higher in teratozoospermic and oligoasthenoteratozoospermic groups. Significant difference in chromatin packing and DNA fragmentation index was observed in these abnormal groups compared with normal. Similarly significant correlation was also seen between abnormal motility parameters and DNA fragmentation index in asthenozoospermic group compared with normal. Conclusions Specific abnormal morphological forms have higher incidence of chromatin packing abnormalities and DNA fragmentation. Using these sperms in ICSI might have an impact on fertilization, embryo development and abortion rates. These can be selectively avoided during ICSI procedure to improve ART outcome.

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Section: Discussionmentioning

confidence: 99%

CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation

Sivanarayana

Krishna

Prakash³

et al. 2012

J Assist Reprod Genet

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“…All oocytes in both cycles showed one polar body and no pronuclei. In the three subsequent cycles, we applied IMSI [20] combined with AOA using fresh semen (Table 1). In the third cycle, 7/8 fresh MII donor oocytes were fertilized, and five developing embryos were obtained; two embryos were transferred at Day 3 (one with nine cells of unequal size, <10 % fragmentation; and one with six cells of unequal size, 0 % fragmentation); one non-compacted embryo was vitrified at Day 4 (eight cells of equal size, 0 % fragmentation).…”

Section: Semen Characteristics and Clinical Parameters Of Icsi Cyclesmentioning

confidence: 99%

PLCζ disruption with complete fertilization failure in normozoospermia

Durbán¹,

Barragán²,

Colodrón³

et al. 2015

J Assist Reprod Genet

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Purpose Intracytoplasmic sperm injection (ICSI) is widely used to achieve fertilization in the presence of severe male factor, resulting in high fertilization rates. Nevertheless, 1-3 % of couples experience complete fertilization failure after ICSI. When a male factor is identified, assisted oocyte activation (AOA) can help overcome fertilization failures. The objective of this study is to describe a case of repeated complete fertilization failures after ICSI with donor oocytes, and to investigate the molecular and functional aspects of phospholipase C zeta (PLCζ) protein in the patient semen. Methods The patient was a normozoospermic male who had previously fathered, through natural conception, four children by a different partner. Molecular and functional analysis of sperm-specific PLCζ in the patient and control samples by means of gene sequencing, immunocytochemistry, Western blot, mouse oocyte activation test (MOAT), and mouse oocyte calcium analysis (MOCA) were used.Results PLCζ expression levels and distribution were significantly disrupted, although MOAT and MOCA did not indicate a decrease in activation ability. Conclusions Normozoospermic males can have disrupted expression and distribution of PLCζ, and reduced activation ability after ICSI in human oocytes, despite their normal activation potential in functional testing using mouse oocytes. Discrepancy among molecular and functional data might exist, as mutations in the gene sequence may not be the only cause of alteration in PLCζ protein related to activation failures.

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“…49 The study design used by Bartoov's group was able to exclude the special sperm preparation technique needed for IMSI as the reason for higher implantation and pregnancy rates and lower miscarriage rates. 50 Performing diagnostic MSOME or therapeutic IMSI has several clinical limitations: (1) significant time and effort is needed for IMSI, because sperm selection can take several hours; (2) expertise and training is needed for these techniques; and (3) the cost of upgrading laboratory equipment to perform IMSI is significant. 50 Taking into account these issues, a diagnostic MSOME is advised in advance of IMSI to determine in which patients IMSI is likely to improve ICSI outcomes.…”

Section: Reactive Oxygen Speciesmentioning

confidence: 99%

An Assessment of New Sperm Tests for Male Infertility

Natali

Turek

2011

Urology

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The routine semen analysis, although used for more than 50 years, fails to accurately distinguish between fertile and infertile men. As a consequence, many tests of sperm function (TSF) have been developed. This review discusses both older and newer diagnostic TSF. It outlines the principles underlying each assay and reviews aggregate clinical data to determine its current relevance and utility. It concludes that the relevance of many older TSF is questionable, with the wide acceptance of intracytoplasmic sperm injection (ICSI). Newer TSF have the potential to deliver more clinically relevant information but require more extensive study to better understand their predictive role in the ICSI era. UROLOGY 77: 1027-1034, 2011. © 2011 Elsevier Inc. All rights reserved.I n addition to the medical history and physical examination, the conventional semen analysis has been an essential laboratory test for the evaluation of male fertility for at least 50 years. However, the idea that fertility can be defined by threshold values of semen parameters is a concept that is fundamentally flawed. 1 Although not a true measure of fertility, the semen analysis, if abnormal, suggests that the probability of achieving fertility is lower than normal. 2 Because of biological variability, two semen analyses are generally needed, performed with two to three days of sexual abstinence and evaluated in a standardized fashion. 2 Recognized normal values for semen parameters are given in Table 1 in agreement with the latest recommendations by the World Health Organization (WHO). 2 Traditionally, (except for the latest WHO recommendations), these references are derived by expert consensus and not by prospective clinical trials and thus their true relationship to male fertility is unclear. In addition, the definition of what constitutes "normal" semen parameters is constantly challenged. A meta-analysis of 29 US studies of semen quality from 9612 fertile, or presumably fertile, men suggested that a sperm concentration of 98 million/mL is normal. 3 Although sperm motility is considered the "best" predictor of fertility, normal sperm motility ranges from 53% to 62%. 4 Thus, simply deriving "normal" semen parameters has been a prohibitively lengthy and inconclusive process to date.Finally, other biological variables affect the clinical utility of the semen analysis. In addition to wide intraindividual variation, seasonal 5 and geographic variation 4 further complicate the performance of the semen analysis as a fertility measure. As examples, within-subject, interejaculate coefficients of variation for sperm concentration and motility are estimated at 44.7% and 15% in one study. 1 Thus, even with excellent quality control, wide biological variation in semen quality profoundly challenges the notion that the conventional semen analysis can accurately assess male fertility. OLDER ADJUNCTIVE SPERM TESTSBecause of the need to more precisely characterize normal fertile semen, adjunctive semen testing has become popular (Figure 1). The concept behind de...

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The morphological normalcy of the sperm nucleus and pregnancy rate of intracytoplasmic injection with morphologically selected sperm

Abstract: Implantation and pregnancy by ICSI is associated with morphological nuclear normalcy of sperm. Sperm with a morphologically abnormal nucleus usually have low fertility potential, but some with certain nuclear abnormalities may still be able to produce pregnancy following ICSI.

Cited by 273 publications

References 16 publications

CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation

CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation

PLCζ disruption with complete fertilization failure in normozoospermia

An Assessment of New Sperm Tests for Male Infertility

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