THE MORPHOLOGICAL CHARACTERISTICS OF ADAPTED LAMTORO (Leucaena leucocephala CV TARRAMBA) pH 3.4 THAT PRODUCED BY 40GY GAMMA RAY IRRADIATION BASED ON DIFFERENCES OF CYTOKININS IN TISSUE CULTURE

Abstract

ABSTRAKLamtoro merupakan tanaman leguminosa pohon dengan kandungan protein tinggi yang memiliki tingkat adaptasi lingkungan yang luas terhadap cekaman kering, tetapi kemampuan adaptasi lamtoro terhadap cekaman masam relatif terbatas. Pemutasian lamtoro melalui iradiasi sinar gamma dosis 40 Gy pada tingkat kalus melalui kultur jaringan menghasilkan kandidat kalus lamtoro teradaptasi asam pada pH 3.4. Penelitian ini bertujuan untuk mengukur karakteristik morfologi kalus lamtoro cv Tarramba teradaptasi pH 3.4 hasil iradiasi sinar gamma 40Gy berdasaarkan perbedaan sumber sitokinin (kinetin, BAP, TDZ) pada kultur jaringan. Rancangan penelitian adalah RAL dengan 3 perlakuan perbedaan sumber sitokinin (kinetin, BAP dan TDZ) dan 10 ulangan. Hasil penelitian menunjukkan sumber sitokinin TDZ 0.5 ppm memberikan respon diameter kalus terbaik serta mampu menghasilkan tekstur kalus yang remah, tetapi menunjukkan respon warna kalus yang cenderung hijau muda. Kata kunci: Leucaena leucocephala cv Tarramba, morfologi kalus, teradaptasi pH 3.4, iradiasi sinar gamma, sitokininABSTRACT Lamtoro, a legume tree plant with high protein content that has a wide range of environmental adaptation to dry stress, but relatively limited to acid stress. Mutation of lamtoro through 40 Gy gamma ray irradiation on callus culture produces lamtoro callus candidates adapted with pH 3.4. Aim of the study was to measure the morphological characteristics of adapted lamtoro cv Tarramba pH 3.4 that produced by 40Gy gamma ray irradiation based on differences of cytokinins in tissue culture. The study used complete randomized design with three treatments of different sources of cytokines i.e. kinetin, BAP and TDZ with 10 replications. The results showed that 0.5 ppm TDZ was the best treatment in callus diameter, and produced crumbly callus texture, but showed callus color response that tended to be light green.