The Molecular Detection of Human Bocavirus (HBoV) in Colorectal Tissue with Malignant and Non-Malignant Lesions

Abstract

Background:Colorectal cancer (CRC) as a worldwide human health concern is identified being a multifactorial subject that infection with specific viral particles such as oncogenic viruses is research interest. Human bocavirus (HBoV) as a recent isolated virus has been investigated in many respiratory and enteric diseases but rare studies evaluates it in tissue specimens especially in cancerous sections. The aim of this study was to detect the presence of HBoV genome and its genotyping in CRC patient’s tissue and compare the result with matched healthy control group tissue.Method:in this retrospective case-control study, CRC cases were sporadic and non-familial cancerous while control subjects had healthy or non-malignant lesions in colon tissue. A conventional-PCR performed by specific primers for HBoV VP1 gene. After sequencing of positive PCR products, raw data used for trimming and alignment by bioinformatics software CLC Main Workbench 5 and MEGA5. SPSS v.22 used for statistical calculations.Result:a total of 157 subjects were participated that 66 were diagnosed as CRC cases and 91 were non-CRC colon tissue as control group that matched by the cases. The mean age (y) ± standard deviation of each case and control groups were 59.35±14.48 and 57.21±14.66, respectively. PCR results showed there were 1.3% (2/157) HBoV positive (of each groups one was positive). Sequencing analysis showed all were HBoV-1 genotype.Conclusion:our study showed there are low rate of HBoV genome in Iranian CRC and non-CRC colon tissue. Furthermore, the predominant genotype in our studied subsets were HBoV-1 according to phylogenetic analysis.

T84 Monolayer Cell Cultures Support Productive HBoV and HSV-1 Replication and Enable In Vitro Co-Infection Studies

“…In addition to these commonly known facts and the observation by several groups that HBoV is frequently detected as a co-pathogen [5,6], our group has made a series of clinical observations that support the hypothesis that the HBoV replication cycle is influenced by herpesviruses. First, we and others have identified HBoV in up to 25% of colorectal and lung cancers and later also in up to 60% of tonsillar tumors [7][8][9][10][11]. In concert with the fact that HBoV establishes a covalently closed circular DNA [12,13], we assumed that it may persist, which was supported (if not confirmed) by a clinical case in which HBoV cccDNA was detected over more than six months, while in the phases with clinical symptoms of cough the cccDNA was found as a relaxed circle, whereas in the asymptomatic phases the cccDNA was coiled or supercoiled [14].…”

Epidemiology of Human Bocavirus in the Middle East and North Africa: Systematic Review

“…In Egypt, Abdel-Moneim et al (2016) studied the presence of HBoV in colorectal cancer patients and found that among one hundred and one patients, twenty-four of them (23.8%) were positive for HBoV [15]. Moreover, Niya et al (2018) used a case versus control population to detect the presence of the HBoV genome in colorectal cancer patients' tissue and compared the result with matched healthy control group tissue; HBoV was detected in one patient from each group, with a total prevalence of 1.3% [31].…”

Plasma Changes of Branched-Chain Amino Acid in Patients with Esophageal Cancer

“…11,12 Furthermore, AAs play a role in energy production, the synthesis of nucleosides, and the maintenance of redox balance in cancer cells. 13 Many types of cancer are accompanied by significant changes in the metabolism 14 of AAs according to the tumor demands and its interaction with the host cell. In this context, BCAAs supplements have been studied as a way to improve protein synthesis.…”

“…Many types of cancer are accompanied by significant changes in the metabolism 14 of AAs according to the tumor demands and its interaction with the host cell. In this context, BCAAs supplements have been studied as a way to improve protein synthesis.…”