The Molecular Control of Adipogenesis, with Special Reference to Lymphatic Pathology

Rosen, Evan D.

doi:10.1111/j.1749-6632.2002.tb04875.x

Cited by 74 publications

(58 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These findings strongly suggest that stromal cells and blood vessels play key roles in adipogenesis and obesity. However, little is known about how adipogenesis proceeds in vivo or the significance and mechanism of the interactions between stromal cells, vascular cells, and adipocytes (17,18). This is in part because adipose tissue is largely comprised of adipocytes containing large lipid droplets.…”

mentioning

confidence: 99%

Adipogenesis in Obesity Requires Close Interplay Between Differentiating Adipocytes, Stromal Cells, and Blood Vessels

et al. 2007

View full text Add to dashboard Cite

OBJECTIVE-The expansion of adipose tissue mass seen in obesity involves both hyperplasia and hypertrophy of adipocytes. However, little is known about how adipocytes, adipocyte precursors, blood vessels, and stromal cells interact with one another to achieve adipogenesis. RESEARCH DESIGN AND METHODS-We have developed a confocal microscopy-based method of three-dimensional visualization of intact living adipose tissue that enabled us to simultaneously evaluate angiogenesis and adipogenesis in db/db mice.RESULTS-We found that adipocyte differentiation takes place within cell clusters (which we designated adipogenic/angiogenic cell clusters) that contain multiple cell types, including endothelial cells and stromal cells that express CD34 and CD68 and bind lectin. There were close spatial and temporal interrelationships between blood vessel formation and adipogenesis, and the sprouting of new blood vessels from preexisting vasculature was coupled to adipocyte differentiation. CD34 ϩ CD68 ϩ lectin-binding cells could clearly be distinguished from CD34 Ϫ CD68 ϩ macrophages, which were scattered in the stroma and did not bind lectin. Adipogenic/angiogenic cell clusters can morphologically and immunohistochemically be distinguished from crownlike structures frequently seen in the late stages of adipose tissue obesity. Administration of anti-vascular endothelial growth factor (VEGF) antibodies inhibited not only angiogenesis but also the formation of adipogenic/angiogenic cell clusters, indicating that the coupling of adipogenesis and angiogenesis is essential for differentiation of adipocytes in obesity and that VEGF is a key mediator of that process. CONCLUSIONS-Living tissue imaging techniques providenovel evidence of the dynamic interactions between differentiating adipocytes, stromal cells, and angiogenesis in living obese adipose tissue. Diabetes

show abstract

mentioning

confidence: 99%

Adipogenesis in Obesity Requires Close Interplay Between Differentiating Adipocytes, Stromal Cells, and Blood Vessels

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Peroxisome proliferator-activator receptor gamma (PPARγ) is a transcription factor and a pivotal regulator of adipocyte differentiation (1). It contains an N-terminal transactivation domain (activation function 1, AF1), a DNA binding domain (DBD), and a C-terminal ligand binding domain (LBD, which harbors the liganddependent transactivation domain AF2).…”

mentioning

confidence: 99%

c-Abl tyrosine kinase promotes adipocyte differentiation by targeting PPAR-gamma 2

Keshet

Kraitshtein

Shanzer

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Adipocyte differentiation, or adipogenesis, is a complex and highly regulated process. A recent proteomic analysis has predicted that the nonreceptor tyrosine kinase Abelson murine leukemia viral oncogene (c-Abl) is a putative key regulator of adipogenesis, but the underlying mechanism remained obscure. We found that c-Abl was activated during the early phase of mouse 3T3-L1 preadipocyte differentiation. Moreover, c-Abl activity was essential and its inhibition blocked differentiation to mature adipocytes. c-Abl directly controlled the expression and activity of the master adipogenic regulator peroxisome proliferator-activator receptor gamma 2 (PPARγ2). PPARγ2 physically associated with c-Abl and underwent phosphorylation on two tyrosine residues within its regulatory activation function 1 (AF1) domain. We demonstrated that this process positively regulates PPARγ2 stability and adipogenesis. Remarkably, c-Abl binding to PPARγ2 required the Pro12 residue that has a phenotypically wellstudied common human genetic proline 12 alanine substitution (Pro12Ala) polymorphism. Our findings establish a critical role for c-Abl in adipocyte differentiation and explain the behavior of the known Pro12Ala polymorphism.

show abstract

“…Notably, lymph fluid and chylomicrons can stimulate adipocyte differentiation (Nougues et al 1993;Rosen 2002). This finding is consistent with the fact that chronic lymphedema is often associated with tissue fibrosis and accumulation of fat and adipose tissues (Rockson 2000(Rockson , 2009.…”

mentioning

confidence: 99%

The New Era of the Lymphatic System: No Longer Secondary to the Blood Vascular System

Choi

Lee²,

Hong

2012

Cold Spring Harbor Perspectives in Medicine

119

107

View full text Add to dashboard Cite

The blood and lymphatic systems are the two major circulatory systems in our body. Although the blood system has been studied extensively, the lymphatic system has received much less scientific and medical attention because of its elusive morphology and mysterious pathophysiology. However, a series of landmark discoveries made in the past decade has begun to change the previous misconception of the lymphatic system to be secondary to the more essential blood vascular system. In this article, we review the current understanding of the development and pathology of the lymphatic system. We hope to convince readers that the lymphatic system is no less essential than the blood circulatory system for human health and well-being.

show abstract

The Molecular Control of Adipogenesis, with Special Reference to Lymphatic Pathology

Cited by 74 publications

References 89 publications

Adipogenesis in Obesity Requires Close Interplay Between Differentiating Adipocytes, Stromal Cells, and Blood Vessels

Adipogenesis in Obesity Requires Close Interplay Between Differentiating Adipocytes, Stromal Cells, and Blood Vessels

c-Abl tyrosine kinase promotes adipocyte differentiation by targeting PPAR-gamma 2

The New Era of the Lymphatic System: No Longer Secondary to the Blood Vascular System

Contact Info

Product

Resources

About