The Molecular Characterization of Echinococcus granulosus Isolates from Sheep in Kırıkkale Region

Yildiran, Fatma Azize Budak; Yıldız, Kader; Çakir, Şükran; Gazyağci, Aycan Nuriye

doi:10.9775/kvfd.2009.638

Cited by 3 publications

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“…All examined 28 isolates were E. granulosus s. s. (G1-G3). In concordance with the other studies 4,[8][9][10][11][12][19][20][21][22][23][24] , E. granulosus s. s. (G1-G3) was predominant in sheep in Kilis province of Turkey. This is the first study about molecular characterization of sheep isolates of E. granulosus in Kilis province.…”

Section: Figsupporting

confidence: 89%

“…In Turkey, different molecular techniques such as PCR, PCR-RFLP (restriction fragment length polymorphism), RAPD-PCR (random amplified polymorphic DNA), PZR-SSCP (single stranded conformation polymorphism) and DNA sequencing have been used to detect different strains of E. granulosus 8,9,[19][20][21] .…”

Section: Discussionmentioning

confidence: 99%

“…Studies on E. granulosus isolates from animals and humans have revealed that the predominant strain is G1 both in humans and in animals, and also G1/G3, G3, G6, G7 strains exist in Turkey 4,[8][9][10][11][12][19][20][21][22][23][24] .…”

Section: Figmentioning

confidence: 99%

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Kilis İlinde Echinococcus granulosus’un Koyun İzolatlarının Moleküler Karakterizasyonu

Ütük¹,

Pişkin²,

Dalkiliç³

2009

Kafkas Univ Vet Fak Derg

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SummaryCystic echinococcosis is one of the most important helminthozoonosis both in Turkey and all around world. Polymerase Chain Reaction (PCR) amplification of mitochondrial 12S rRNA (mt-12S rRNA) and small subunit ribosomal RNA (rrnS) genes were used for molecular characterization of 28 isolates of Echinococcus granulosus obtained from 19 sheep in Kilis province. And also partial mitochondrial cytochrome c oxidase subunit I (mt-CO1) genes of randomly selected two sheep isolates were amplified and sequenced. At the end of the study, all sheep isolates were detected as E. granulosus sensu stricto (G1-G3 complex). Keywords: Echinococcus granulosus, Kilis, Sheep, PCR Kilis İlinde Echinococcus granulosus'un Koyun İzolatlarının Moleküler Karakterizasyonu ÖzetKistik ekinokokkozis Türkiye'de ve dünyada en önemli helmintozoonozlardan biridir. Kilis ilindeki 19 koyundan elde edilen 28 Echinococcus granulosus izolatının moleküler karakterizasyonu için mitokondrial 12S rRNA (mt-12S rRNA) ve küçük altünite ribosomal RNA (rrnS) genleri polimeraz zincir reaksiyonu (PZR) ile çoğaltıldı. Ayrıca rastgele seçilen iki koyun izolatının mitokondrial sitokrom c oksidaz altünite 1 (mt-CO1) geni kısmi olarak çoğaltılıp dizi analizi yapıldı. Çalışma sonucunda tüm koyun izolatları E. granulosus sensu stricto (G1-G3 kompleks) olarak belirlendi.

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Section: Figsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

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Kilis İlinde Echinococcus granulosus’un Koyun İzolatlarının Moleküler Karakterizasyonu

Ütük¹,

Pişkin²,

Dalkiliç³

2009

Kafkas Univ Vet Fak Derg

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“…Despite its public health impact, relatively little informations avaliable on the presence of the different genotypes (strains, species) of E. granulosus. In Turkey, many studies have been performed regarding the prevalence of the disease in sheep and cattle but only few studies have been performed about genetic characterization of Echinococcus variants [3][4][5][6][7][8][9] . Therefore the aim of the present study was to provide molecular characterization of E. granulosus isolates from cattle in Manisa province of Turkey.…”

Section: Introductionmentioning

confidence: 99%

Manisa İlinde Echinococcus granulosus’un Sığır İzolatlarının Moleküler Analizi

Altıntaş¹,

Öztatlıcı²,

Altıntaş³

et al. 2013

Kafkas Univ Vet Fak Derg

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SummaryEchinococcus granulosus is the causative agent of cystic echinococcosis (CE) in humans and many domestic animals, and still one of the most important global health problem in the world and in Turkey. Infection with metacestode causes severe illness and high economic losses. Several strains of Echinococcus have been identified based on the epidemiological and biological characteristics of strains. In this study, a total of 18 individual hydatid cyst samples from cattle were examined. They were obtained from central slaughterhouse in the province of Manisa/Turkey between 2010-2012. The total genomic DNA (gDNA) was extracted using RTA-DNA Isolation Kit (Gebze/Kocaeli, Turkey) according to manufacturer instructions from protoscoleces and cystic germinal membranes. The aim of this study was to provide molecular characterization of E. granulosus isolates which were obtained from cattles by using polymerase chain reaction (PCR) in Manisa province of Turkey. After PCR, to investigate the genetic characteristics of isolates, deoxyribonucleic acid sequencing of the mitochondrial cytochrome c oxidase subunit 1 (CO1) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (NAD1) genes were performed with ABI Prism Genetic Analyzer 3100 instrument. As a result of our study, all (18) cattle isolates were detected as E. granulosus sensu stricto (G1-G3 complex). This is the first molecular study report genotyping of Echinococcus isolates from cattle in Manisa province. Keywords: Echinococcus granulosus, Cattle, Genotyping, PCR, DNA Sequence, Turkey Manisa İlinde Echinococcus granulosus'un Sığır İzolatlarının Moleküler Analizi ÖzetEchinococcus granulosus insanda ve birçok evcil hayvanda kistik ekinokokkozise (KE) neden olan etkendir ve hala dünyada ve Türkiye'de en önemli sağlık problemlerinden biridir. Metasestodlarla infeksiyon şiddetli hastalıklara ve yüksek ekonomik kayıplara neden olur. Bazı Echinococcus suşları, suşların epidemiyolojik ve biyolojik karakteristiklerine dayanarak tanımlanmaktadır. Çalışmamızda sığırlardan elde edilen toplam 18 örnek incelenmiştir. Örnekler 2010-2012 yılları arasında Manisa merkez mezbahasından elde edilmiştir. Total genomik DNA (gDNA) üretici firmanın talimatları doğrultusunda protoskoleks ve kistik germinal membranlardan RTA-DNA İzolasyon Kiti kullanılarak (Gebze/Kocaeli, Türkiye) izole edilmiştir. Bu çalışmanın amacı, Türkiye'de Manisa ilindeki sığırlardan elde edilen E. granulosus izolatlarının Polimeraz Zincir Reaksiyonu (PZR) ile moleküler karakterizasyonunun elde edilmesidir. PZR'dan sonra, izolatların genetik karakteristiklerini araştırmak için mitokondrial sitokrom c oksidaz alt ünite 1 (CO1) ve nikotinamid adenin dinükleotit dehidrogenaz alt ünite 1 (NAD1) genleri deoksiribonükleik asit dizileme ile ABI Prism Genetik Analizör 3100 cihazıyla çalışıldı. Çalışmamızın sonucu olarak, tüm (18) sığır izolatları E. granulosus sensu stricto (G1-G3 kompleksi) olarak teşhis edildi. Bu çalışma Manisa ilindeki sığırlardan elde edilen Echinococcus izolatlarının ilk moleküler genotipl...

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Evaluation of the Molecular Properties of Echinococcus granulosus Isolates from Various Hosts in Şanlıurfa Province

Günbegi Çalışkan,

Yıldız Zeyrek

2024

Journal of Anatolian Environmental and Animal Sciences

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Cystic echinococcosis (CE), which is raised by the larvae of Echinococcus granulosus (E. granulosus), is single of the world's most significant zoonoses, affecting both humans and animals. In this labor, it was the goal to perform genotyping of hydatid cyst stocks collected from various regions of Şanlıurfa and diverse middle hosts by molecular methods. A total of 197 hydatid cyst samples were obtained, including 40 pre-diagnosed human samples with paraffin, Türkiye 140 sheep, and 17 cattle samples. After microscopic examination, 33 human isolates and 80 sheep cyst are regarded fertile because they contain more than 250 protoscolex and/or scolex hooks. PCR-RFLP technique was used to determine the genetics of fertile isolates by analyzing the ribosomal DNA internal transcribed spacer gene 1 (rDNA-ITS1) region of the samples. As a result of having the same band profile, it was found that all of the isolates were common sheep strain (G1). Echinococcus granulosus G1 strain was the reference material. This study demonstrated that the local sheep lineage of E. granulosus is the dominant genotype in Şanlıurfa province. It is thought to lead to further work on the epidemiology and ecology of parasites in animals in this region and neighboring countries.

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The Molecular Characterization of Echinococcus granulosus Isolates from Sheep in Kırıkkale Region

Cited by 3 publications

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Kilis İlinde Echinococcus granulosus’un Koyun İzolatlarının Moleküler Karakterizasyonu

Kilis İlinde Echinococcus granulosus’un Koyun İzolatlarının Moleküler Karakterizasyonu

Manisa İlinde Echinococcus granulosus’un Sığır İzolatlarının Moleküler Analizi

Evaluation of the Molecular Properties of Echinococcus granulosus Isolates from Various Hosts in Şanlıurfa Province

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