The molecular characterization and immune protection of microneme 2 of Eimeria acervulina

Zhang, Zhenchao; Liu, Lianrui; Huang, Jingsheng; Wang, Shuai; Lu, Mingmin; Song, Xiaoling; Xu, Lixin; Ren, Yan; Li, Xiangrui

doi:10.1016/j.vetpar.2015.10.028

Cited by 21 publications

(23 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Part of the identified antigens were also identified as immunodominant antigens in previous studies, including MIC3, pyruvate kinase, enolase, actin, aspartyl proteinase, 14-3-3 protein, lactate dehydrogenase and so on [29–31]. Some of the identified antigens have been demonstrated to be able to confer protection against Eimeria challenge, such as lactate dehydrogenase [32], microneme 2 [33], microneme 7 [34] and so on. Therefore, the immunodominant antigens identified in this study have the potential for conferring protection against Eimeria challenge.…”

Section: Discussionmentioning

confidence: 98%

Identification of common immunodominant antigens of Eimeria tenella, Eimeria acervulina and Eimeria maxima by immunoproteomic analysis

et al. 2017

Self Cite

View full text Add to dashboard Cite

Clinical chicken coccidiosis is mostly caused by simultaneous infection of several Eimeria species, and host immunity against Eimeria is species-specific. It is urgent to identify common immunodominant antigen of Eimeria for developing multivalent anticoccidial vaccines. In this study, sporozoite proteins of Eimeria tenella, Eimeria acervulina and Eimeria maxima were analyzed by two-dimensional electrophoresis (2DE). Western bot analysis was performed on the yielded 2DE gel using antisera of E. tenella E. acervulina and E. maxima respectively. Next, the detected immunodominant spots were identified by comparing the data from MALDI-TOF-MS/MS with available databases. Finally, Eimeria common antigens were identified by comparing amino acid sequence between the three Eimeria species. The results showed that analysis by 2DE of sporozoite proteins detected 629, 626 and 632 protein spots from E. tenella, E. acervulina and E. maxima respectively. Western bot analysis revealed 50 (E. tenella), 64 (E. acervulina) and 57 (E. maxima) immunodominant spots from the sporozoite 2DE gels of the three Eimeria species. The immunodominant spots were identified as 33, 27 and 25 immunodominant antigens of E. tenella, E. acervulina and E. maxima respectively. Fifty-four immunodominant proteins were identified as 18 ortholog proteins among the three Eimeria species. Finally, 5 of the 18 ortholog proteins were identified as common immunodominant antigens including elongation factor 2 (EF-2), 14-3-3 protein, ubiquitin-conjugating enzyme domain-containing protein (UCE) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). In conclusion, our results not only provide Eimeria sporozoite immunodominant antigen map and additional immunodominant antigens, but also common immunodominant antigens for developing multivalent anticoccidial vaccines.

show abstract

Section: Discussionmentioning

confidence: 98%

Identification of common immunodominant antigens of Eimeria tenella, Eimeria acervulina and Eimeria maxima by immunoproteomic analysis

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, the MIC2 and MIC5 of E. acervulina were also identified and reported (Zhang et al. , ).…”

mentioning

confidence: 92%

“…The gene sequences of E. tenella MIC1 (AF032905.1), MIC2 (KC333870.1), MIC3 (AY512382.1), MIC4 (AJ306453.2), MIC5 (AJ245536.1) and AMA1 (JN032081.1), E. maxima MIC2 (FR718971.1), MIC3 (FR718972.1), MIC5 (FR718974.1) and MIC7 (FR718975.1) and E. necatrix MIC5 (EU335049.1) were published in GenBank. In addition, the MIC2 and MIC5 of E. acervulina were also identified and reported (Zhang et al 2014b(Zhang et al , 2016. Tomley et al (1996) indicated that EtMIC2, which is secreted from the host-parasite interface and intimately involved in host cell invasion, has been found within the microneme organelles of E. tenella sporozoites and merozoites.…”

Section: Introductionmentioning

confidence: 98%

“…The EtMIC2 protein has been reported that has good immunogenicity in stimulating host immune responses as vaccine and could provide high specific antibody response, reducing the oocyst output and cecal lesions (Zhang et al 2014a). Zhang et al (2014bZhang et al ( , 2016 found EaMIC2 and EaMIC5 protein were expressed in sporozoites and merozoites stages of E. acervulina and could be an effective candidate for the development of new vaccine against this parasite.…”

Section: Introductionmentioning

confidence: 99%

“…Zhang et al. (, ) found EaMIC2 and EaMIC5 protein were expressed in sporozoites and merozoites stages of E. acervulina and could be an effective candidate for the development of new vaccine against this parasite.…”

mentioning

confidence: 99%

See 2 more Smart Citations

The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina

Zhang

Liu

Yang

et al. 2016

J Eukaryotic Microbiology

Self Cite

View full text Add to dashboard Cite

The gene of Eimeria acervulina microneme protein 3 (EaMIC3) was cloned and characterized. According to the conserved sequence, the 3'- and 5'-ends of EaMIC3 were amplified by the rapid amplification of cDNA ends (RACE). The full length cDNA of this gene was obtained by overlapping the sequences of 3'- and 5'-extremities and amplification by reverse transcription PCR. The sequence analysis revealed that the opening reading frame (ORF) of EaMIC3 was 2,607 bp and encoded a protein of 868 amino acids with 93.04 kDa. Western blotting assay showed that the recombinant protein was successfully recognized by the sera of chickens experimentally infected with E. acervulina, whereas the native protein in the somatic extract of sporozoites was as well detected by sera from rats immunized with the recombinant protein of EaMIC3. Immunofluorescence analysis indicated that EaMIC3 was expressed in the sporozoites and merozoites stages of E. acervulina. Animal challenge experiments demonstrated that the recombinant protein of EaMIC3 could significantly increase the average body weight gains, decrease the mean lesion scores and the oocyst outputs of the immunized chickens and presented anticoccidial index (ACI) more than 165. Moreover, EaMIC3 protein produced significantly high level of IgG antibody, IFN-γ, IL-10, IL-17 TGF-β, CD4 , and CD8 .

show abstract

Evaluation of immunoprotective effects of recombinant proteins and DNA vaccines derived from Eimeria tenella surface antigen 6 and 15 in vivo

et al. 2021

View full text Add to dashboard Cite

The molecular characterization and immune protection of microneme 2 of Eimeria acervulina

Cited by 21 publications

References 35 publications

Identification of common immunodominant antigens of Eimeria tenella, Eimeria acervulina and Eimeria maxima by immunoproteomic analysis

Identification of common immunodominant antigens of Eimeria tenella, Eimeria acervulina and Eimeria maxima by immunoproteomic analysis

The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina

Evaluation of immunoprotective effects of recombinant proteins and DNA vaccines derived from Eimeria tenella surface antigen 6 and 15 in vivo

Contact Info

Product

Resources

About