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Late blight (LB), caused by the oomycete pathogen Phytophthora infestans, is a devastating disease of potato that is necessary to control by regularly treatment with fungicides. Silicon (Si) has been used to enhance plant resistance against a broad range of bacterial and fungal pathogens; however, the enhanced LB resistance and the molecular mechanisms involving the plant hormone pathways remain unclear. In this study, Si treatment of potato plants was found to enhance LB resistance in both detached leaves and living plants accompanied by induction of reactive oxygen species (ROS) production and pathogenesis-related genes expression. Regarding the hormone pathways involved in Si-mediated LB resistance, we found a rapidly increased content of ethylene (ET) 15 min after spraying with Si. Increased jasmonic acid (JA) and JA-Ile and decreased salicylic acid (SA) were identified in plants at 1 day after spraying with Si and an additional 1 day after P. infestans EC1 infection. Furthermore, pretreatment with Me-JA enhanced resistance to EC1, while pretreatment with DIECA, an inhibitor of JA synthesis, enhanced the susceptibility and attenuated the Si-mediated resistance to LB. Consistent with these hormonal alterations, Si-mediated LB resistance was significantly attenuated in StETR1-, StEIN2-, StAOS-, StOPR3-, StNPR1-, and StHSP90-repressed plants but not in StCOI1- and StSID2-repressed plants using virus-induced gene silencing (VIGS). The Si-mediated accumulation of JA/JA-Ile was significantly attenuated in StETR1-, StEIN2-, StOPR3- and StHSP90-VIGS plants but not in StCOI1-, StSID2- and StNPR1-VIGS plants. Overall, we reveal that Si can be used as a putative alternative to fungicides to control LB, and conclude that Si-mediated LB resistance is dependent on the ET/JA-signaling pathways in a StHSP90- and StNPR1-dependent manner.

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Background：Coccidiosis is an intestinal parasitic disease that causes huge economic losses to the poultry industry globally. At present, the primary control strategy is administration of anticoccidial drugs with feed. However, overuse of anticoccidials, such as sulfachloropyrazine sodium (SC), has resulted in an increase in the emergence of drug resistance.Methods: We aimed to evaluate coccidiosis prevalence and SC resistance in field isolates to provide reasonable guidance on the use of anticoccidial drugs in the Hubei and Henan provinces. We collected 318 fresh fecal samples from 137 chicken farms. We used internal transcribed spacer 1 (ITS1) sequence of ribosomal DNA to identify the species from 94 samples that were collected from different farms and to assess drug resistance.Results: As shown by genus-specific PCR results, the positivity rate of Eimeria was 97.17 % (309/318), and the most common species were E. mitis (66.67%), E. tenella (46.86%), and E. necatrix (41.51%). Animal experiment demonstrated that 25 strains were completely resistant to SC, among which 16 were from Henan and nine were from Hubei. Twenty-four strains were partially resistant, among which 8 and 16 strains were identified from Hubei and Henan, respectively.Conclusions: In summary, these data indicated that chicken coccidia is ubiquitous and SC resistance is widespread, in the Hubei and Henan provinces. The results provide important insights into the control of chicken coccidiosis in this region.