Evaluation of immunoprotective effects of recombinant proteins and DNA vaccines derived from Eimeria tenella surface antigen 6 and 15 in vivo

Geng, Tiantian; Luo, Liangshun; Wang, Yale; Shen, Bang; Fang, Rui; Hu, Min; Zhao, Junlong; Zhou, Yanqin

doi:10.1007/s00436-021-07364-9

Cited by 11 publications

(6 citation statements)

References 24 publications

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“…The expression of SAG is differentially regulated between oocyst/zygotes and second-generation cleavage stages, with only one being specifically expressed in the zygotes, a few in both stages, and most in the second generation cleavage ( Tabares et al, 2004 ), with the SAG B gene being specifically expressed in the second-generation cleavage peak expression in the second generation of cleistogamy. In recent years, many researchers have investigated the immunogenicity of SAG proteins, such as SAG1 ( Vo et al, 2021 ), SAG2, SAG4 ( Zhao et al, 2020b ), SAG6, SAG15 ( Geng et al, 2022 ), SAG16, SAG22, SAG23 ( Song et al, 2021 ; Zhao et al, 2021 ), among which SAG1, SAG4, SAG15, and SAG22 have candidate antigenic genes to be anticoccidial vaccines. In general, protective antigens will prefer proteins expressed in the intracellular phase or in the metabolically active phase of the worm, whereas SAG22 belongs to the SAG B gene, which is capable of efficient expression in the second generation of cleavers.…”

Section: Discussionmentioning

confidence: 99%

Construction of recombinant SAG22 Bacillus subtilis and its effect on immune protection of coccidia

Zifan,

Chaojun,

Qiaoli

et al. 2023

Poultry Science

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Section: Discussionmentioning

confidence: 99%

Construction of recombinant SAG22 Bacillus subtilis and its effect on immune protection of coccidia

Zifan,

Chaojun,

Qiaoli

et al. 2023

Poultry Science

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“…Detailed studies in Eimeria genomics with novel tools have revealed many microbial proteins that are considered as the candidates of vaccine antigens ( 47 , 48 ). Many Eimeria antigens are utilized to construct the anticoccidial vaccines, such as surface antigen ( 49 ), apical membrane antigen ( 11 , 50 ), microneme ( 51 , 52 ), rhoptry, profilin ( 53 ) etc. Although the antigens mentioned above have been proved to be effective in eliciting host immunity against the Eimeria species, these antigens could not provide fully immunoprotections against coccidiosis.…”

Section: Discussionmentioning

confidence: 99%

Actin depolymerizing factor-based nanomaterials: A novel strategy to enhance E. mitis-specific immunity

et al. 2022

Front. Immunol.

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The epidemic of avian coccidiosis seriously threatens the animals’ welfare and the economic gains of the poultry industry. Widespread in avian coccidiosis, Eimeria mitis (E. mitis) could obviously impair the production performance of the infected chickens. So far, few effective vaccines targeting E. mitis have been reported, and the nanovaccines composed of nanospheres captured our particular attention. At the present study, we construct two kinds of nanospheres carrying the recombinant E. mitis actin depolymerizing factor (rEmADF), then the characterization was then analyzed. After safety evaluation, the protective efficacy of rEmADF along with its nanospheres were investigated in chickens. The promoted secretions of antibodies and cytokines, as well as the enhanced percentages of CD4+ and CD8+ T cells were evaluated by the ELISA and flow cytometry assay. In addition, the absolute quantitative real-time PCR (qPCR) assay implied that vaccinations with rEmADF-entrapped nanospheres could significantly reduce the replications of E. mitis in feces. Compared with the rEmADF-loaded chitosan (EmADF-CS) nanospheres, the PLGA nanospheres carrying rEmADF (EmADF-PLGA nanosphers) were more effective in up-regulating weight efficiency of animals and generated equally ability in controlling E. mitis burdens in feces, suggesting the PLGA and CS nanospheres loaded with rEmADF were the satisfactory nanovaccines for E. mitis defense. Collectively, nanomaterials may be an effective antigen delivery system that could help recombinant E. mitis actin depolymerizing factor to enhance immunoprotections in chicken against the infections of E. mitis.

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“…Vaccines are promising alternatives to anticoccidial drugs for disease control, but live vaccines require to replicate parasites to induce the host immune system, which takes a considerable amount of time and results in a high risk of triggering subclinical coccidiosis 6 . DNA vaccines, as the next‐generation vaccines, have been frequently developed against Eimeria infection, but no commercially available vaccines have reached the market 7–9 . To develop effective methods for the control of chicken coccidiosis, it is necessary to deeply comprehend the possible mechanism of the immune response.…”

Section: Introductionmentioning

confidence: 99%

“…6 DNA vaccines, as the next-generation vaccines, have been frequently developed against Eimeria infection, but no commercially available vaccines have reached the market. [7][8][9] To develop effective methods for the control of chicken coccidiosis, it is necessary to deeply comprehend the possible mechanism of the immune response.Song Wang, Danni Wang and Yilin Bai contributed to the work equally and should be regarded as co-first authors.…”

mentioning

confidence: 99%

Expression of Toll‐like receptors and host defence peptides in the cecum of chicken challenged with Eimeria tenella

Wang,

Bai

et al. 2024

Parasite Immunology

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Chicken coccidiosis, caused by Eimeria protozoa, affects poultry farming. Toll‐like receptors (TLRs) and host defence peptides (HDPs) help host innate immune responses to eliminate invading pathogens, but their roles in Eimeria tenella infection remain poorly understood. Herein, 14‐day‐old chickens were treated orally with 50,000 E. tenella oocysts and the cecum was dissected at different timepoints. mRNA expression of 10 chicken TLRs (chTLRs) and five HDPs was measured by quantitative real‐time PCR. chTLR7 and chTLR15 were upregulated significantly at 3 h post‐infection while other chTLRs were downregulated (p < .05). chTLR1a, chTLR1b, chTLR2b and chTLR4 peaked at 36 h post‐infection, chTLR3, chTLR5 and chTLR15 peaked at 72 h post‐infection and chTLR21 expression was highest among chTLRs, peaking at 48 h post‐infection (p < 0.05). For HDPs, cathelicidin (CATH) 1 to 3 and B1 peaked at 48 h post‐infection, liver‐expressed antimicrobial peptide 2 peaked at 96 h post‐infection, and CATH 2 expression was highest among HDPs. CATH2 and CATH3 were markedly upregulated at 3 h post‐infection (p < .05). The results provide insight into innate immune molecules during E. tenella infection in chicken, and indicate that innate immune responses may mediate resistance to chicken coccidiosis.

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Evaluation of immunoprotective effects of recombinant proteins and DNA vaccines derived from Eimeria tenella surface antigen 6 and 15 in vivo

Cited by 11 publications

References 24 publications

Construction of recombinant SAG22 Bacillus subtilis and its effect on immune protection of coccidia

Construction of recombinant SAG22 Bacillus subtilis and its effect on immune protection of coccidia

Actin depolymerizing factor-based nanomaterials: A novel strategy to enhance E. mitis-specific immunity

Expression of Toll‐like receptors and host defence peptides in the cecum of chicken challenged with Eimeria tenella

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