The Microbial Metabolism of Cinnamic Acid

Sulfolane (tetrahydrothiophene-1,1-dioxide) is used in the Sulfinol process for natural gas sweetening. At many sour-gas processing plants spills, landfills and leakage from unlined surface storage ponds have contaminated groundwaters with sulfolane. Due to its high water solubility and mobility in aquifers, sulfolane poses a risk for off-site contamination. This study investigated the aerobic biodegradation of sulfolane by two mixed microbial enrichment cultures and by three bacterial isolates. Sulfolane served as the sole C, S and energy source for these cultures. In the two mixed cultures, 60% and 80% of the sulfolane C was recovered as CO2, whereas in cultures of the three isolates only 40-42% of the substrate C was recovered as CO,. In the mixed cultures, 81% and 97% of the sulfolane S was converted to sulfate, and in the pure isolates, 55-90% of the substrate S was converted to sulfate. Thus, the mixed cultures were capable of greater mineralization than the pure isolates. One isolate, strain WP1, was identified using a combination of 16S rRNA gene sequencing, physiological traits and cell morphology. WP1 was determined to be most similar to Varioivorax paradoxus.

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“…For example, a Pseudomonas sp. reduces cinnamic acid (3-phenylpropenoic acid) to 3-phenylpropionic acid (Blakley and Simpson 1964).…”

Section: Discussionmentioning

confidence: 99%

Sulfolane degradation by mixed cultures and a bacterial isolate identified as a Variovorax sp.

Greene

Beatty

Fedorak

2000

Archives of Microbiology

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“…It has been found that many kinds of root exudates and decaying residues are phytotoxic as the result of allelochemicals contained in root exudates and decaying residues that have been isolated and identified as organic acids, especially phenolic acids, such as cinnamic, vanillic, coumaric, and ferulic (Lee et al 2006;Ohno et al 2001;Asao et al 2003). Phenolic acids frequently occurring in plants, such as ferulic, p-coumaric and syringic acids, are released during the aerobic catabolism of lignin (Kuwahara 1980;Blackley and Simpson 1964).…”

Section: Abbreviationsmentioning

confidence: 99%

Physiological and biochemical responses of in vitro Fusarium oxysporum f.sp. niveum to benzoic acid

Wang

Zhang

et al. 2009

Folia Microbiol

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The allelopathic potential of an artificially applied allelochemical, benzoic acid, on in vitro Fusarium oxysporum f.sp. niveum (a soil-borne pathogen causing watermelon wilt) was evaluated. Benzoic acid strongly inhibited its growth, sporulation and conidia germination, whereas it stimulated virulence factors of this pathogen. The biomass was reduced by 83-96 % and the conidia germinating rate and conidia production rate were decreased by 100 % at a concentration of >200 mg/L. However, phytopathogenic enzyme activities and mycotoxin production were stimulated with an increase of 10.2-1250 % for enzyme activities and 610-2630 % for mycotoxin yield.

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“…Chemicals. propionate (DHPP) were synthesized by the procedures described by Blakley & Simpson (1964) and Burlingame & Chapman (1983). Other chemicals were purchased from Tokyo Kasei or Sigma.…”

Section: Methodsmentioning

confidence: 99%

Genetic organization and characteristics of the 3-(3-hydroxyphenyl)propionic acid degradation pathway of Comamonas testosteroni TA441

et al. 1999

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Comamonas testosteroni TA441 degrades 3-(3-hydroxyphenyl)propionate (3HPP) via the meta pathway. A gene cluster required for degradation of 3HPP was cloned from strain TA441 and sequenced. The genes encoding six catabolic enzymes, a flavin-type hydroxylase (mhpA), extradiol dioxygenase (mhpB), 2-keto-4-pentenoate hydratase (mhpD), acetaldehyde dehydrogenase (acylating) (mhpF), 4-hydroxy-2-ketovalerate aldolase (mhpE) and the meta cleavage compound hydrolase (mhpC), were found in this cluster, encoded in this order. mhpD and mhpF were separated by two genes, orf4 and orf5, which were not necessary for growth on 3HPP. The gene mhpR, encoding a putative transcriptional activator of the IclR family, was located adjacent to mhpA in the opposite orientation. Disruption of the mhpB or mhpR genes affected growth on 3HPP or trans-3-hydroxycinnamate. The mhpB and mhpC gene products showed high specificity for 3-(2,3-dihydroxyphenyl)propionate (DHPP) and the meta cleavage compound produced from DHPP, respectively.

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The Microbial Metabolism of Cinnamic Acid

Cited by 58 publications

References 12 publications

Sulfolane degradation by mixed cultures and a bacterial isolate identified as a Variovorax sp.

Sulfolane degradation by mixed cultures and a bacterial isolate identified as a Variovorax sp.

Physiological and biochemical responses of in vitro Fusarium oxysporum f.sp. niveum to benzoic acid

Genetic organization and characteristics of the 3-(3-hydroxyphenyl)propionic acid degradation pathway of Comamonas testosteroni TA441

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