Physiological and biochemical responses of in vitro Fusarium oxysporum f.sp. niveum to benzoic acid

Wu, Hong‐sheng; Wang, Y.; Zhang, C. Y.; Gu, Minghong; Liu, Y. X.; Chen, G.; Wang, J. H.; Tang, Zhu; Mao, Ze-Shen; Shen, Qirong

doi:10.1007/s12223-009-0017-6

Cited by 18 publications

(13 citation statements)

References 40 publications

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“…The findings of this work were similar to those previously reported by EI Modafar and EI Boustani (2001), who found that cell wall-bound phenolic acids (p-hydroxybenzoic, p-coumaric, ferulic, and sinapic acids) strongly reduced the mycelial growth of F. Other studies also showed that the hyphal growth and biomass of F. oxysporum f. sp. Niveum were strongly inhibited by cinnamic (Wu et al 2008b), p-hydroxybenzoic (Wu et al 2010b), succinic (Wu et al 2011), ferulic (Wu et al 2010a), and benzoic (Wu et al 2009c) acids from the root exudates and decaying residues of watermelon. However, some of these studies found that conidial germination on plates and conidial production in liquid cultures were completely inhibited at the highest concentrations of cinnamic (Wu et al 2008b), p-hydroxybenzoic (Wu et al 2010b), and benzoic (Wu et al 2009c) acids, which was not in agreement with the results of the present study, in which PA showed no statistically significant inhibition of the conidial germination and sporulation of F. oxysporum, even at the highest concentration (1 mM; Fig.…”

Section: Discussionmentioning

confidence: 99%

In vitro study of the growth, development and pathogenicity responses of Fusarium oxysporum to phthalic acid, an autotoxin from Lanzhou lily

Yang

Wang

et al. 2015

World J Microbiol Biotechnol

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Continuous monoculture of Lanzhou lily (Lilium davidii var. unicolor Cotton) results in frequent incidence of fusarium wilt caused by Fusarium oxysporum. Phthalic acid (PA), a principal autotoxin from root exudates of Lanzhou lily, is involved in soil sickness by inducing autotoxicity. The aim of this study was to evaluate the direct allelopathic effects of PA on the growth, development and pathogenicity of F. oxysporum in vitro based on an ecologically relevant soil concentration. The results showed that PA slightly but not significantly inhibited the colony growth (mycelial growth) and fungal biomass of F. oxysporum at low concentrations ranging from 0.05 to 0.5 mM, and significantly inhibited the colony growth at the highest concentration (1 mM). None of the PA concentrations tested significantly inhibited the conidial germination and sporulation of F. oxysporum in liquid medium. However, mycotoxin (fusaric acid) yield and pathogenesis-related hydrolytic enzyme (protease, pectinase, cellulase, and amylase) activities were significantly stimulated in liquid cultures of F. oxysporum containing PA at ≥ 0.25 mM. We conclude that PA at a soil level (i.e. 0.25 mM) is involved in plant-pathogen allelopathy as a stimulator of mycotoxin production and hydrolytic enzyme activities in F. oxysporum, which is possibly one of the mechanisms responsible for promoting the wilt disease of lily.

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Section: Discussionmentioning

confidence: 99%

In vitro study of the growth, development and pathogenicity responses of Fusarium oxysporum to phthalic acid, an autotoxin from Lanzhou lily

Yang

Wang

et al. 2015

World J Microbiol Biotechnol

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“…Aromatic compounds have been reported to stimulate conidial germination of F. oxysporum f. sp. niveum at low concentrations and/or to inhibit germination at higher concentrations (Wu et al ., , b, c, , b, c, d, ). Therefore, germination rates of the wild‐type and cmle deletion mutant KO6 were assessed in the presence of various aromatic compounds.…”

Section: Resultsmentioning

confidence: 99%

“…Wu et al . (, d) described a concentration‐dependent effect of sinapic acid and benzoic acid on conidial germination of F. oxysporum f. sp. niveum .…”

Section: Discussionmentioning

confidence: 99%

Degradation of aromatic compounds through the β‐ketoadipate pathway is required for pathogenicity of the tomato wilt pathogen Fusarium oxysporum f. sp. lycopersici

Michielse

Reijnen

Olivain

et al. 2012

Molecular Plant Pathology

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Summary Plant roots react to pathogen attack by the activation of general and systemic resistance, including the lignification of cell walls and increased release of phenolic compounds in root exudate. Some fungi have the capacity to degrade lignin using ligninolytic extracellular peroxidases and laccases. Aromatic lignin breakdown products are further catabolized via the β‐ketoadipate pathway. In this study, we investigated the role of 3‐carboxy‐cis,cis‐muconate lactonizing enzyme (CMLE), an enzyme of the β‐ketoadipate pathway, in the pathogenicity of Fusarium oxysporum f. sp. lycopersici towards its host, tomato. As expected, the cmle deletion mutant cannot catabolize phenolic compounds known to be degraded via the β‐ketoadipate pathway. In addition, the mutant is impaired in root invasion and is nonpathogenic, even though it shows normal superficial root colonization. We hypothesize that the β‐ketoadipate pathway in plant‐pathogenic, soil‐borne fungi is necessary to degrade phenolic compounds in root exudate and/or inside roots in order to establish disease.

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“…Cellulase and pectinase hydrolyze and cleave the polymers, such as cellulose and pectin, that comprise the important components of plant cell wall. This renders the plant more vulnerable, and fungi are more likely to infect it [24,25]. Fusaric acid is an important virulence factor that causes plants to wilt, which affects plant growth in many ways [26,27].…”

Section: Wheat Intercropping Alleviates the Autotoxicity Of Salicylicmentioning

confidence: 99%

Salicylic acid has allelopathy, but wheat and faba bean intercropping can alleviate faba bean fusarium wilt under salicylic acid stress.

Lv¹,

Liu²,

Ling³

et al. 2021

Preprint

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BackgroundThe goals of this study were to evaluate the role of salicylic acid in the continuous cropping obstacle of faba bean (Vicia faba L.) and explore how intercropping with wheat alleviates these obstacles. We designed a hydroponic pot experiment to study the effects of exogenous salicylic acid on the occurrence of Fusarium wilt, seedling growth, physiological resistance of faba bean and pathogenicity of Fusarium oxysporum f. sp. fabae (FOF).ResultsThe results showed that salicylic acid significantly increased the incidence and disease index of faba bean, inhibited the growth of seedlings and reduced the physiological resistance of faba bean. An in vitro study of FOF found that salicylic acid increased the ability of the organism to produce fusaric acid, cellulase and pectinase, which increased the susceptibility of faba bean to Fusarium wilt. Interestingly, intercropping with wheat significantly reduced the exudation of salicylic acid from the faba bean root system, which directly reduced the deleterious effects of salicylic acid. Alternatively, intercropping also increased the ability of faba bean to defend itself from the aspect of physiological resistance and indirectly reduced the autotoxicity of salicylic acid.ConclusionsIn conclusion, we found that salicylic acid, as an autotoxic substance, deleteriously affected the growth of faba bean, but intercropping with wheat could alleviate its autotoxicity. This finding suggests the existence of an important mechanism in which intercropping alleviates the obstacles in continuous cropping and controls Fusarium wilt.

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Physiological and biochemical responses of in vitro Fusarium oxysporum f.sp. niveum to benzoic acid

Cited by 18 publications

References 40 publications

In vitro study of the growth, development and pathogenicity responses of Fusarium oxysporum to phthalic acid, an autotoxin from Lanzhou lily

In vitro study of the growth, development and pathogenicity responses of Fusarium oxysporum to phthalic acid, an autotoxin from Lanzhou lily

Degradation of aromatic compounds through the β‐ketoadipate pathway is required for pathogenicity of the tomato wilt pathogen Fusarium oxysporum f. sp. lycopersici

Salicylic acid has allelopathy, but wheat and faba bean intercropping can alleviate faba bean fusarium wilt under salicylic acid stress.

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