The Metal Dependence of Bacillus subtilis Phytase

Kerovuo, Janne; Lappalainen, Ilkka; Reinikainen, Tapani

doi:10.1006/bbrc.2000.2131

Cited by 61 publications

(45 citation statements)

References 31 publications

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“…Although commercial production of phytases is currently focused on fungal histidine acid phytases from Aspergillus species (1,11), bacterial phytases from Bacillus species are an alternative to fungal enzymes because of their high thermal stability, calcium-phytate complex substrate specificity, pH profile, and proteolysis resistance (15,19,27). In contrast to the Aspergillus phytases, Bacillus phytases are specific for phytate.…”

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confidence: 99%

Expression of a Bacillus Phytase C Gene in Pichia pastoris and Properties of the Recombinant Enzyme

Guerrero-Olazarán

Rodríguez-Blanco

Carreon-Treviño

et al. 2010

Appl Environ Microbiol

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The cloning and expression of a native gene encoding a Bacillus subtilis phytase using Pichia pastoris as the host is described. In addition, the influence of N-glycosylation on the biochemical properties of the B. subtilis phytase, the influence of pH on the thermostability of the recombinant and native B. subtilis phytases, and the resistance of both phytases to shrimp digestive enzymes and porcine trypsin are also described. After 48 h of methanol induction in shake flasks, a selected recombinant strain produced and secreted 0.82 U/ml (71 mg/liter) recombinant phytase. This phytase was N-glycosylated, had a molecular mass of 39 kDa after N-deglycosylation, exhibited activity within a pH range of 2.5 to 9 and at temperatures of 25 to 70°C, had high residual activity (85% ؎ 2%) after 10 min of heat treatment at 80°C and pH 5.5 in the presence of 5 mM CaCl 2 , and was resistant to shrimp digestive enzymes and porcine trypsin. Although the recombinant Bacillus phytase had pH and temperature activity profiles that were similar to those of the corresponding nonglycosylated native phytase, the thermal stabilities of the recombinant and native phytases were different, although both were calcium concentration and pH dependent.

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confidence: 99%

Expression of a Bacillus Phytase C Gene in Pichia pastoris and Properties of the Recombinant Enzyme

Guerrero-Olazarán

Rodríguez-Blanco

Carreon-Treviño

et al. 2010

Appl Environ Microbiol

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“…To date, most of phytases have not been described as metalloenzymes, except that some Bacillus sp. phytase required calcium for active conformation and thermostability, and readily lost the enzyme activity through its removal by EDTA (Choi et al, 2001; Kerovuo et al, 2000). BUN1 phytase had high activity for phytate, but none or low activity on other phosphorylated compounds including p-nitrophenylphosphate, a representative substrate for acid phosphatase (Table 3).…”

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confidence: 99%

General Properties of Phytase Produced by Fluorescent Pseudomonas sp. BUN1

Cho¹

2009

Journal of Animal Science and Technology

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)과 같은 금속 2 가이온에 대하여 그 효소활성이 강하게 억제되었다. 또한 BUN1 균주의 효소생산 배지 (PSM) [0.5% sodium phytate, 0.5% (NH4)2SO4, 0.5% KCl, 0.01% MgSO4 ․ 7H2O, 0.01% CaCl2 ․ 2H2O, 0.01% NaCl, 0.001% FeSO4 ․ 7H2O, 0.001% MnSO4 ․ 4H2O; pH 6.5]에 탄소원으로서 옥수수전분 (corn starch)의 첨가는 조사된 다른 탄소 배지원에 비하여 현저하게 파이테이즈 (phytase) 생산을 촉진시켰다.

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“…Asam fitat memiliki fosfor bermuatan negatif yang besar sehingga asam fitat mampu berikatan dengan banyak kation divalen, protein, dan karbohidrat.Garam dari asam fitat disebut sebagai phytates (Grafs et al, 1987;Kerovuo et al, 2000).Fosfatyang berikatan pada asam fitat terdiri dari dua posisi yaitu aksial dan ekuatorial, terdapat lima gugus Pberada dalam posisi equator dan satu gugus dengan posisi aksial (Bohn et al, 2008).…”

Section: Struktur Dan Sifat Asam Fitatunclassified

Fitat dan fitase : dampak pada hewan ternak

Yanuartono

Nururrozi

Indarjulianto

2016

JIIP

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ABSTRACT:In countries with high plant biodiversity such as Indonesia, the availability of food of plant origin is very diverse. The presence of anti-nutrients in plants would potentially cause problems in cattle if not managed properly. phytic acid is one anti nutritional factor that have a role in disrupting the health and productivity The term phytate refers to the molecule phytic acid, which generally acts as a chelate to Mg, Ca, Na, and K, and in some cases protein and carbohydrates. Seeds of cereals, legumes and oilseed plant which is used as animal feed usually contains a lot of phytic acid which can cause a decline in nutritional value. However, with a variety of processing methods, levels of phytic acid in animal feed can be reduced or even eliminated. In addition to the processing method, the method of adding phytase enzyme may also be done to improve the nutritional value of the animal feed ingredients.

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The Metal Dependence of Bacillus subtilis Phytase

Cited by 61 publications

References 31 publications

Expression of a Bacillus Phytase C Gene in Pichia pastoris and Properties of the Recombinant Enzyme

Expression of a Bacillus Phytase C Gene in Pichia pastoris and Properties of the Recombinant Enzyme

General Properties of Phytase Produced by Fluorescent Pseudomonas sp. BUN1

Fitat dan fitase : dampak pada hewan ternak

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