The metabolomic approach to the comparative analysis of wild and cultivated species of oats (Avena L.)

Лоскутов, И. Г.; Шеленга, Т. В.; Конарев, А. В.; Шаварда, А. Л.; Blinova, E. V.; Dzubenko, N. I.

doi:10.1134/s2079059717050136

Cited by 24 publications

(19 citation statements)

References 6 publications

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“…Metabolomic profiles of grain from Ae. tauschii accessions were studied at the Department of Biochemistry and Molecular Biology of VIR (in 5 biological and 3 analytical replications) (Loskutov et al, 2017). The grains were cleaned of glumes and ground; 50 mg of the flour of an accession were homogenized with 500 μL of methanol, and the sample was kept at 5-6 °C for 30 days.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, nonspecific metabolomic analysis has found wide application for plant species phenotyping and resistance stu dies, which provides a unique opportunity to scan a wide range of compounds that make up the metabolomic profile in the source material and give an objective assessment (using metabolomic markers) of the plant's response to environmental factors (Konarev et al, 2015). This approach is increasingly used to identify individual metabolites or their groups that can characterize the protective status of the studied object, which makes it possible to identify accessions resistant to environmental stressors (Taji et al, 2006;Chakraborty, Newton, 2011;Valitova et al, 2016;Loskutov et al, 2017). Currently, metabolomic profiles of various crops from the VIR collection are investigated.…”

Section: Introductionmentioning

confidence: 99%

“…Wild and cultivated forms of oats resistant and susceptible to Fusarium were studied, and significant differences among them were shown for a number of compounds (acylglycerols, nonproteinogenic amino acids, galactinol, etc.) (Loskutov et al, 2017(Loskutov et al, , 2019. Total screening of wild forms of various crops will make it possible to shape a model "metabolomic profile of a resistant cultivar".…”

Section: Introductionmentioning

confidence: 99%

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Metabolomic approach to search for fungal resistant forms of <i>Aegilops tauschii</i> Coss. from the VIR collection

et al. 2020

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Broadening of the genetic diversity of donors of resistance to biotic environmental factors is a challenging problem concerning Triticum L., which can be solved by using wild relatives of wheat, in particular, Aegilops tauschii Coss., in breeding programs. This species, believed to be the donor of D genome of common wheat (T. aestivum L.), is a source of some traits important for breeding. This greatly facilitates the possibility of crossing Ae. tauschii with common wheat. Aegilops L. species are donors of effective genes for resistance to fungal diseases in wheat. For instance, genes that determine resistance to rust agents in common wheat were successfully introgressed from Ae. tauschii into the genome of T. aestivum L. The aim of our study was to identify differences in metabolomic profiles of Ae. tauschii forms (genotypes), resistant or susceptible to such fungal pathogens as Puccinia triticina f. sp. tritici and Erysiphe graminis f. sp. tritici. These indicators may be used as biochemical markers of resistance. A comparative analysis of groups of Ae. tauschii accessions showed that metabolomic profiles of the forms with or without resistance to fungal pathogens differed significantly in the contents of nonproteinogenic amino acids, polyols, phytosterols, acylglycerols, mono- and oligosaccharides, glycosides, phenolic compounds (hydroquinone, kempferol), etc. This fact was consistent with the previously obtained data on the relationship between Fusarium resistance in oats (Avena sativa L.) and certain components of the metabolomic profile, such as acylglycerols, nonproteinogenic amino acids, galactinol, etc. Thus, our studies once again confirmed the possibility and effectiveness of the use of metabolomic analysis for screening the genetic diversity of accessions in the VIR collection, of Ae. tauschii in particular, in order to identify forms with a set of compounds in their metabolomic profile, which characterize them as resistant. Ae. tauschii accessions with a high content of pipecolic acids, acylglycerols, galactinol, stigmasterol, glycerol, azelaic and pyrogallic acids, campesterol, hydroquinone, etc., can be used for creating wheat and triticale cultivars with high resistance to fungal pathogens causing powdery mildew, brown rust, and yellow rust.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metabolomic approach to search for fungal resistant forms of <i>Aegilops tauschii</i> Coss. from the VIR collection

et al. 2020

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“…In sorghum, there are currently no particular metabolomics reports on grains, but, metabolic profiling (GC-MS) in leaf tissues of 11 inbreeding lines shows clear associations of both primary and secondary metabolites (such as chromogenic and shikimic acid) with photosynthesis and biomass [92]. In oat, metabolomics studies (GC-MS) using grains from 12 wild and 10 cultivated oat accessions recommend some wild species as potential sources of nutritional traits for breeding purposes and identifies metabolites that vary during domestication or differentiates wild oats from cultivars [93]. In addition, comparative metabolomic profiling (GC-MS) finds that, as compared with wheat and barley, oat, together with rye, contains relatively higher levels of the most phenolic acids [94].…”

Section: Other Cropsmentioning

confidence: 99%

Promoting Human Nutrition and Health through Plant Metabolomics: Current Status and Challenges

Sun

Chen

Hong

et al. 2020

Biology

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Plant metabolomics plays important roles in both basic and applied studies regarding all aspects of plant development and stress responses. With the improvement of living standards, people need high quality and safe food supplies. Thus, understanding the pathways involved in the biosynthesis of nutritionally and healthily associated metabolites in plants and the responses to plant-derived biohazards in humans is of equal importance to meet people’s needs. For each, metabolomics has a vital role to play, which is discussed in detail in this review. In addition, the core elements of plant metabolomics are highlighted, researches on metabolomics-based crop improvement for nutrition and safety are summarized, metabolomics studies on plant natural products including traditional Chinese medicine (TCM) for health promotion are briefly presented. Challenges are discussed and future perspectives of metabolomics as one of the most important tools to promote human nutrition and health are proposed.

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“…Собранное и сохраняемое в национальных генных банках и центрах генетическое разнообразие (ГР) с момента поступления в коллекцию становилось объектом комплексного изучения, в том числе по признакам качества [1][2][3][4][5][6][7]. Сейчас, когда качество признано приоритетным направлением селекции, нельзя не вспомнить о первопроходческой роли основателя ВИР -Н.И.…”

Section: Introductionunclassified

Modern approach of structuring the variety diversity of the naked and covered forms of cultural oats (Avena sativa L.)

Loskutov

Градиславович²,

Шеленга

et al. 2020

Ecological genetics

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Background. Structuring and phenotyping genetic diversity is an important aspect of the work with breeding sources and materials. In the Introduction, the authors pointed out the role of N.I. Vavilovs scientific foresight in defining the topical trend in researching the genetic diversity of a crop, particularly the analysis of its biochemical composition. As the target of their research, the authors chose biochemical characters identifiable in the process of metabolomic analysis conducted by means of gas chromatography with mass spectrometry. Materials and methods. The object was the grain of naked and covered forms of common oat (Avena sativa L.) from the collection held by the Oat, Rye and Barley Genetic Resources Department of VIR. The analysis of oil fatty acid content and metabolomic research were performed using the method of gas-liquid chromatography with mass spectrometry on the chromatograph Agilent 6850 (USA). Results. The obtained metabolomic spectra which reflected the metabolomic status of genotypes of various ecogeographic origin were compared among themselves using statistical (principal component) analysis methods. The results of the comparison are discussed by referring to the most important groups of metabolites significant for forming the traits of resistance to stressors as well as the characters related to food qualities of grain products. Special attention has been paid to biologically active compounds determining the functional value of the products for human nutrition: the sum of phenolics in covered forms is five times higher than that in naked ones and the content of glycine amino acid in covered forms is five times higher than in naked grain, with a similar proportion in the content of organic acids, sugars, etc. Conclusion. Differences between metabolomic profiles of naked and covered forms have been detected and statistically verified. Accessions with the most optimal nutritional composition have been identified for food purposes and for the development of resistance to biotic and abiotic environmental stresses.

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The metabolomic approach to the comparative analysis of wild and cultivated species of oats (Avena L.)

Cited by 24 publications

References 6 publications

Metabolomic approach to search for fungal resistant forms of <i>Aegilops tauschii</i> Coss. from the VIR collection

Metabolomic approach to search for fungal resistant forms of <i>Aegilops tauschii</i> Coss. from the VIR collection

Promoting Human Nutrition and Health through Plant Metabolomics: Current Status and Challenges

Modern approach of structuring the variety diversity of the naked and covered forms of cultural oats (Avena sativa L.)

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