The Metabolism of Normal and Leukemic Leukocytes

Glover, Eugene C.

doi:10.1001/archinte.1930.00140130049004

Cited by 19 publications

(11 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Table I shows the high glycolytic rate characteristic of leukemia. This observation has also been made by Glover, Daland and Schmitz (40), Falcon-Lesses (3), and Lepine and Boulud (41). However, as pointed out by Burger (9), Falcon-Lesses (3) and Schmitz and Glover (4) in their observations of chronic myelogenous leukemia and lymphatic leukemia, the amount of sugar glycolyzed is not necessarily in proportion to the total number of leucocytes present.…”

supporting

confidence: 78%

“…In this connection it is interesting to note that Harrop (34), Morawitz (38) and Derra (39) have reported the increased use of oxygen by immature erythrocytes. No attempt was made in this study to correlate the rate of glycolysis and the oxygen consumption but, in view of the conclusions drawn by Glover, Daland and Schmitz (40) regarding the oxygen utilization and glycolytic rate of normal and leukemic leucocytes, such a study is desirable.…”

mentioning

confidence: 99%

See 1 more Smart Citation

A Study of Glycolysis

Barer

1931

J. Clin. Invest.

View full text Add to dashboard Cite

supporting

confidence: 78%

mentioning

confidence: 99%

A Study of Glycolysis

Barer

1931

J. Clin. Invest.

View full text Add to dashboard Cite

“…En étudiant la glycolyse de cellules leucémiques, Barron et H a r r o p (4) ont observé un effet de foule à des concentrations cellulaires élevées (environ 100 X 10e leucocytes par ml) ; en revanche, avec des cellules analogues, G lover et al (13) n'ont pas trouvé d'effet de foule pour des concentrations cellulaires variant de 23,5 à 749 X 10® leucocytes par ml. R asio et Conard (21) rapportent qu'avec des polynucléaires d'exsudats péritonéaux de rat, la consommation de glucose est linéaire à la concentration cellulaire, entre 2 et 20xI06 leucocytes par ml.…”

unclassified

Effet de l’insuline et de quelques autres facteurs sur la glycolyse des leucocytes humains mesurée in vitro

Antonioli¹,

Felber²,

A³

1967

Acta Haematol

View full text Add to dashboard Cite

“…These studies have yielded contradictory results. Daland and Isaacs (4) as well as Glover, Daland and Schmitz (5) concluded that mature leucocytes consume greater amounts of oxygen than do immature white cells. The latter investigators (5) also concluded that the glycolytic function of the leucocytes, at least in whole blood, is inversely proportional to the maturity of the cells.…”

mentioning

confidence: 99%

The Metabolism of Leucocytes From Normal and Leukemic Blood

Soffer

Wintrobe²

1932

J. Clin. Invest.

View full text Add to dashboard Cite

Warburg's studies (9) on the metabolism of tumor cells have stimulated great interest in similar studies of the blood corpuscles. Grafe (1) has shown that leucocytes consume considerable amounts of oxygen. Although it had previously been known that they are capable of splitting sugar, it remained for Levene and Meyer (2) to demonstrate their ability to produce lactic acid from dextrose. Maclean and Weir (3), in comparing the glycolytic power of leucocytes and erythrocytes, found that the former have a glycolytic power several hundred times greater than the latter. Metabolic studies have been carried out with a view of determining the oxygen consumption and sugar destruction of leucocytes, and of mature as compared with that of immature white corpuscles. These studies have yielded contradictory results. Daland and Isaacs (4) as well as Glover, Daland and Schmitz (5) concluded that mature leucocytes consume greater amounts of oxygen than do immature white cells. The latter investigators (5) also concluded that the glycolytic function of the leucocytes, at least in whole blood, is inversely proportional to the maturity of the cells. These findings could not be confirmed by Barron and Harrop (6), who emphasized the depressing effect of concentration on both the oxygen consumption and sugar destruction of white blood cells.The object of this paper is to study further, with improved technic, the effect of cell maturity and cell concentration on the metabolic activity of leucocytes. METHODWhole blood containing varying numbers of leucocytes from patients suffering from myeloid and lymphoid leukemia and from cases of leucocytosis due to various causes was studied. Arterial blood was collected in most instances. This was done to avoid the injury to the cells which results from manipulation incidental to aeration of venous blood. Samples were collected in purified heparin and immediately studied. Oxygen consumption was determined in Barcroft-Warburg manometers at 37.5°C., the readings being taken at 5 minute intervals for 30 minutes. Sugar determinations were made immediately before placing the samples in the microrespirometers, and directly after the experiment. The samples to be studied were subjected to as little manipulation as possible and centrifuging was entirely avoided in view of its damaging effects on the respiration and to a lesser extent on the glycolytic activity of the white blood cells, as demonstrated by Fujita (7). Varying 43 661

show abstract

The Metabolism of Normal and Leukemic Leukocytes

Cited by 19 publications

References 24 publications

A Study of Glycolysis

A Study of Glycolysis

Effet de l’insuline et de quelques autres facteurs sur la glycolyse des leucocytes humains mesurée in vitro

The Metabolism of Leucocytes From Normal and Leukemic Blood

Contact Info

Product

Resources

About