Effet de l’insuline et de quelques autres facteurs sur la glycolyse des leucocytes humains mesurée in vitro

Antonioli, J.A.; Felber, J. P.; A, Vannotti

doi:10.1159/000209066

Cited by 5 publications

(6 citation statements)

References 12 publications

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“…That physiologic concentrations (100 ,uU/ml for 4 h) failed to enhance transport supports the conclusion that insulin exerts this classical biologic function only on receptor-bearing T cells. The failure to observe an insulin effect on glucose use in freshly prepared lymphoid tissues is consonant with the bulk of previous work (17,19,20,28,29) although Hofert In contrast to the instulin-directed alterations in the rate of intermediary metabolism of the T cell that have been defined and discussed to this point was the failure to identify insulin effects on lactate concentrations.…”

Section: Resultssupporting

confidence: 59%

Role of insulin in the intermediary metabolism of the activated thymic-derived lymphocyte.

Helderman¹

1981

J. Clin. Invest.

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, lactate production and oxidation, and glucose oxidation from carbon 1-and carbon 6-labeled substrates, it was determined that (a) mitogens such as phytohemagglutinin enhance basal T lymphocyte intermediary metabolism, (b) physiologic concentrations ofinsulin have no impact on the metabolism of unstimulated, cultured, receptornegative lymphocytes, and (c) insulin provided to receptor bearing lymphocytes augments intermediary metabolism above mitogen stimulated levels. The importance of the pentose phosphate shunt pathway for energy metabolism in the stimulated lymphocyte was confirmed. These studies demonstrate that insulin has a classical physiologic role to play in the activated lymphocyte further validating the use of this cell to examine potential receptor defects in disorders of carbohydrate metabolism. By enhancing energy metabolism of stimulated lymphocytes, insulin serves biologic economy and thus may perform its immunoregulatory role.

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Section: Resultssupporting

confidence: 59%

Role of insulin in the intermediary metabolism of the activated thymic-derived lymphocyte.

Helderman¹

1981

J. Clin. Invest.

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“…In accordance with the latter observation, Antonioli et al [2] found that an increase in serum insulin from 4.5 to 11.7 |i.U/ml (elicited within 45 min by an oral glucose load) did not change the rate of glucose utilization or lactate production.…”

Section: Insulin Effectsupporting

confidence: 56%

“…In contrast, neither Antonioli et al [2] nor Esmann [40] were able to demonstrate an effect of insulin in physiological concentrations. However, when prolonged incubation and high insulin concentrations (1 U/ml) were used, a non-specific effect, reproducible by substituting human serum albumin in the same concentration as insulin, could on occasion be elicited for glucose utilization and lactate formation [40].…”

Section: Insulin Effectmentioning

confidence: 88%

“…the decreasing metabolic rate observed on increasing the density of leukocyte suspensions. This phenomenon has been known for many years [5] and it has often [10,53,71,100,103], though not invariably [2] been confirmed. It also occurs in suspensions of lymphocytes and red cells [56].…”

Section: Crowding Effectmentioning

confidence: 95%

“…Only two isozymes of hexokinase are present in normal PMN leukocytes [27], Increasing medium glucose from 4 to 20 mM has been reported not to influence [25] or to cause a 15-25% decrease in glucose utilization [39,75,81], the inhibition being equal in normal and diabetic cells [39]. However, other authors have reported a linear increase in glucose utilization with the logarithm of the glucose concentration in the interval 2.84 to 15.8 mM glucose [2], resulting in an increase in glucose utilization by a factor of 3.6 within this interval. The increased glucose utilization was not associated with a rise in glycogen formation or lactic acid production, and the observation appears untenable.…”

Section: Glucose Utilizationmentioning

confidence: 99%

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The Diabetic Leukocyte

Esmann¹

1972

Enzyme

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The metabolism of polymorphonuclear leukocytes in diabetes is reviewed. The available evidence points towards glucose being freely permeable to the cell membrane. Varying results have been obtained on the rate of glucose utilization and glycolysis, probably due to the different experimental conditions employed by various investigators. In this respect, the crowding effect, i.e. the decrease in metabolic rate observed by increasing the cell concentration, is of special importance. When, however, normal and diabetic cells are incubated in a bicarbonate buffer at the same low cell concentration to avoid the crowding effect, a comparison shows that the glycolysis of diabetic cells is decreased. Glucose-6-phosphate and fructose-6-phosphate accumulate in diabetes, suggesting a decreased activity of phosphofructokinase (EC 2.7.1.11). The increase in glucose-6- phosphate concentration is probably responsible for a small decrease in glucose utilization and a slight increase in pentose cycle activity as well as for an increase in the relative amount of glycogen synthetized. Insulin in vivo corrects the metabolic alterations, whereas there is no convincing evidence of an effect of insulin in physiological concentrations in vitro. The crowding effect has been proposed to be due to an inhibition of the activity of phosphofructokinase by the drop in pH which is caused by the accumulation of lactic acid during the incubation. The absence of the crowding effect in diabetic leukocytes has motivated the suggestion that the sensitivity of phosphofructokinase to changes in pH is compromised in diabetes. The amount of glycogen and its synthesis is markedly reduced in diabetes. The total activity of glycogen synthetase, as well as the ability for D to I transformation, is decreased in diabetic cells and normalized by insulin in vivo, no discernible effect of insulin in vitro being present. The respiration of diabetic leukocytes is normal. No conclusive information on lipid or protein metabolism in diabetic leukocytes is available. The function of leukocytes in inflammation appears to be hampered, but no definite corollary to the impaired carbohydrate metabolism has been produced.

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Hormone receptors: VI. on the nature of the binding of glucagon and insulin to human circulating mononuclear leukocytes

Blecher

Goldstein

1977

Molecular and Cellular Endocrinology

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Effet de l’insuline et de quelques autres facteurs sur la glycolyse des leucocytes humains mesurée in vitro

Cited by 5 publications

References 12 publications

Role of insulin in the intermediary metabolism of the activated thymic-derived lymphocyte.

Role of insulin in the intermediary metabolism of the activated thymic-derived lymphocyte.

The Diabetic Leukocyte

Hormone receptors: VI. on the nature of the binding of glucagon and insulin to human circulating mononuclear leukocytes

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