The Mechanism of Lysis by Phage Studied with Defective Lysogenic Bacteria

Effect of Infection with Phage Lambda on the Synthesis of Protein, RNA and DNA in Escherichia coli

“…Assay of endolysin. The method used was based on that of Jacob & Fuerst (1958). The lytic activity of the suspensions of bacteria disrupted by ultrasonic treatment was tested against bacterial suspensions prepared as follows.…”

Enzybiotics and their Potential Applications in Medicine

“…At the last stage of the cycle, endolysin molecules degrade peptidoglycan, thereby causing lysis of the bacterial cell and ensuring the release of progeny virions (Young et al 2000 ). The term " endolysin " was introduced to the scientifi c literature by F. Jacob and C. R. Fuerst to stress that enzyme molecules act on peptidoglycan from within the bacterial cell in which they are synthesized (Jacob and Fuerst 1958 ). In view of this, it appears that recombinant enzymes acting on the cell wall from outside the cell (e.g., those used for therapeutic purposes) should be referred to as lysins rather than endolysins.…”

Lysozymes, Proteinase K, Bacteriophage E Lysis Proteins, and some Chemical Compounds for Microbial Ghosts Preparation: a Review and Food for Thought

“…The microbes' cell walls after their death could stay longer and resist decaying [4][5][6][7]. However, due to the process of the natural decay, environmental effect, enzymatic activity of other microbes, or any other expected mechanism for a pore or pores formation, natural loss of the cytoplasm or in better words, natural microbial ghosting will be happened.…”

“…Using the heat sensitive promotor and regulator were used for better control. Nevertheless, its main weak point that it is restricted only to the gram-negative bacteria [4,28].…”