The mechanism of action of the antidiuretic peptide Tenmo ADFa in Malpighian tubules of<i>Aedes aegypti</i>

Massaro, Richard C.; Lee, Lenora W.; Patel, Ankit; Wu, Dexiang; Yu, Minshu; Scott, Brett N.; Schooley, David A.; Schegg, Kathleen M.; Beyenbach, Klaus W.

doi:10.1242/jeb.01102

Cited by 37 publications

(23 citation statements)

References 62 publications

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“…Incubating Malpighian tubules with 1μmoll -1 Anoga-DH 31 for 2min significantly increased the intracellular cAMP concentration tenfold to 140pmolmg -1 protein. As the cytoplasmic volume of one A. aegypti Malpighian tubule is on average 27nl (Massaro et al, 2004), the basal cytosolic cAMP concentration was ~0.4μmoll , respectively. Aedeskinin-III had no effect on V-ATPase activity.…”

Section: V-atpase Activities In Malpighian Tubulesmentioning

confidence: 99%

Protein kinase A dependent and independent activation of the V-ATPase in Malpighian tubules ofAedes aegypti.

Tiburcy

Beyenbach

Wieczorek

2012

Journal of Experimental Biology

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Section: V-atpase Activities In Malpighian Tubulesmentioning

confidence: 99%

Protein kinase A dependent and independent activation of the V-ATPase in Malpighian tubules ofAedes aegypti.

Tiburcy

Beyenbach

Wieczorek

2012

Journal of Experimental Biology

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“…Hitherto only other ADFs such as those from the meal beetle Tenebrio molitor (e.g. TenmoADFa) are known to use cGMP as a second messenger for the inhibition of fluid secretion by MTs (Eigenheer et al, 2002;Massaro et al, 2004). When assuming that peptide receptors are as similar as their ligands in crustaceans and insects, which is to date known only for some serotonin and dopamine receptors (Clark et al, 2004;Clark and Baro, 2006;Spitzer et al, 2008), one would probably have to expect G-protein-coupled receptors but cannot exclude membrane-bound guanylyl cyclases as mediators of some of the known physiological effects of ITP isoforms.…”

Section: +mentioning

confidence: 99%

Insect ion transport peptides are derived from alternatively spliced genes and differentially expressed in the central and peripheral nervous system

Dircksen

2009

Journal of Experimental Biology

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“…Currently, there are two proposed routes for transepithelial Cl -secretion in adult Aedes Malpighian tubules: a transcellular pathway through stellate cells and a paracellular shunt between adjacent principal cells (Beyenbach, 2003a;Beyenbach, 2003b;Massaro et al, 2004;Yu and Beyenbach, 2001;Yu and Beyenbach, 2004). Evidence supporting a transcellular Cl -pathway is based upon the characterization of two types of Cl -channels on the apical membrane of stellate cells from adult A. aegypti tubules (O'Connor and and, more recently, leucokinin receptors localized to the stellate cell population in adult Anopheles gambiae tubules .…”

Section: Malpighian Tubulesmentioning

confidence: 99%

P-type Na+/K+-ATPase and V-type H+-ATPase expression patterns in the osmoregulatory organs of larval and adult mosquitoAedes aegypti

Patrick

Aimanova

Sanders

et al. 2006

Journal of Experimental Biology

156

174

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SUMMARY This study describes the expression patterns of P-type Na+/K+-ATPase and V-type H+-ATPase in the larval and adult forms of the mosquito Aedes aegypti and provides insight into their relative importance in ion transport function of key osmoregulatory organs. RT-PCR assays indicate that, at the level of the gene,both ATPases are expressed in all of the osmoregulatory tissues of larvae(midgut, Malpighian tubules, rectum and anal papillae) and adults (stomach,Malpighian tubules, anterior hindgut and rectum). Immunohistochemical studies determined that both ATPases are present in high levels in all the relevant organs, with the exception of the larval rectum (P-type Na+/K+-ATPase only). In larval gastric caeca, ATPase location corresponds to the secretory (basal P-type Na+/K+-ATPase, apical V-type H+-ATPase) and ion-transporting (V-type H+-ATPase on both membranes) regions as previously described. The two ATPases switch membrane location along the length of the larval midgut, indicating three possible regionalizations,whereas the adult stomach has uniform expression of basolateral P-type Na+/K+-ATPase and apical V-type H+-ATPase in each cell. In both larval and adult Malpighian tubules, the distal principal cells exhibit high expression levels of V-type H+-ATPase (apically and cytoplasmically) whereas P-type Na+/K+-ATPase is highly expressed in stellate cells found only in the distal two-thirds of each tubule. By contrast, the proximal principal cells express both P-type Na+/K+-ATPase (basal) and V-type H+-ATPase(apical). These results suggest a functional segregation along the length of the Malpighian tubules based on cell type and region. P-type Na+/K+-ATPase is the only pump apparent in the larval rectum whereas in the larval anal papillae and the adult hindgut (including the anterior hindgut and rectum with rectal pads), P-type Na+/K+-ATPase and V-type H+-ATPase localize to the basal and apical membranes, respectively. We discuss our findings in light of previous physiological and morphological studies and re-examine our current models of ion transport in these two developmental stages of mosquitoes that cope with disparate osmoregulatory challenges.

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The mechanism of action of the antidiuretic peptide Tenmo ADFa in Malpighian tubules ofAedes aegypti

Cited by 37 publications

References 62 publications

Protein kinase A dependent and independent activation of the V-ATPase in Malpighian tubules ofAedes aegypti.

Protein kinase A dependent and independent activation of the V-ATPase in Malpighian tubules ofAedes aegypti.

Insect ion transport peptides are derived from alternatively spliced genes and differentially expressed in the central and peripheral nervous system

P-type Na+/K+-ATPase and V-type H+-ATPase expression patterns in the osmoregulatory organs of larval and adult mosquitoAedes aegypti

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