Insect ion transport peptides are derived from alternatively spliced genes and differentially expressed in the central and peripheral nervous system

Dircksen, Heinrich

doi:10.1242/jeb.026112

Cited by 69 publications

(67 citation statements)

References 92 publications

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“…8). The findings that ITP and ITPL act on a discrete receptor(s) are consistent with the different physiological functions of these neuropeptides (3,12). The calculated EC 50 values of these ligand-receptor combinations (Table 4) are 1 order of magnitude lower than that reported for locust ITP on ileal Cl Ϫ transport (1-2 ϫ 10 Ϫ9 M) (29), which may be due to differences in the examined species and cell types between our cell line and ileal tissue.…”

Section: Discussionsupporting

confidence: 55%

Identification and Characterization of Receptors for Ion Transport Peptide (ITP) and ITP-like (ITPL) in the Silkworm Bombyx mori

Nagai

Mabashi-Asazuma

Nagasawa

et al. 2014

Journal of Biological Chemistry

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Background: Ion transport peptide (ITP) and ITP-like (ITPL) are crustacean hyperglycemic hormone family peptides in insects. Results: Receptors for ITP and ITPL were screened from orphan Bombyx neuropeptide G protein-coupled receptors (BNGRs). Conclusion: BNGR-A2 and -A34 act as ITP receptors, whereas BNGR-A24 functions as an ITPL receptor. Significance: Receptor identifications provide further understandings of biological events by this neuropeptide superfamily.

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Section: Discussionsupporting

confidence: 55%

Identification and Characterization of Receptors for Ion Transport Peptide (ITP) and ITP-like (ITPL) in the Silkworm Bombyx mori

Nagai

Mabashi-Asazuma

Nagasawa

et al. 2014

Journal of Biological Chemistry

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“…However, we suggest that the function of NPF in circadian rhythms needs to be subjected to further investigation, as the knockdown by RNAi was not complete. We additionally suggest that possible future work also includes the examination of the function of other neurotransmitters that are expressed in npf-positive clock neurons, such as the ion transport peptide (ITP) or acetylcholine (Audsley et al, 1992;Dircksen, 2009;Johard et al, 2009), to reveal their role in evening activity and free-running phenotypes that we observed in npf-ablated flies.…”

Section: Discussionmentioning

confidence: 99%

Neuropeptide F immunoreactive clock neurons modify evening locomotor activity and free‐running period in Drosophila melanogaster

Hermann

Yoshii

Dusik

et al. 2012

J of Comparative Neurology

100

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Different subsets of Drosophila melanogaster's clock neurons are characterized by their specific functions in daily locomotor rhythms and the differences in their neurotransmitter composition. We investigated the function of the neuropeptide F (NPF) immunoreactive clock neurons in the rhythmic locomotor behavior of adult flies. We newly identified the fifth s-LN(v) and a subset of the l-LN(v)s as NPF-positive in addition to the three LN(d)s that have been described previously. We then selectively ablated different subsets of NPF-expressing neurons using npfGal4-targeted expression of the cell death gene head involution defective (hid) in combination with cryGal80 and pdfGal80. By analyzing daily locomotor rhythms in these flies, we show that the NPF-positive clock neurons--especially the fifth s-LN(v) and the LN(d)s--are involved in both the control of the free-running period in constant darkness (DD) and the phasing and amplitude of the evening activity in light-dark (LD) cycles. Furthermore, we show that the simultaneous ablation of NPF and pigment dispersing factor (PDF)-immunoreactive neurons has additive effects in LD, resulting in an evening peak phase that is even more advanced in comparison to PDF-ablated flies. We also found that this more advanced evening peak is additionally reduced in amplitude. To putatively assign the observed phenotypes to the action of NPF, we knocked it down in conjunction with PDF using RNA-interference (RNAi) and further suggest a possible role for NPF in the control of the flies' evening activity.

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“…Indeed, neuronal fibers positive for serotonin and several neuropeptides have been described in its anterior portion, including dromyosuppressin, adipokinetic hormone, and, possibly, allatostatin-C and FMRFamide (or an FMRFamide-like peptide, such as sNPF) (24,99,110,128). Fibers positive for pigment-dispersing hormone, ion transport peptide, and proctolin have also been observed in both the larval and/or adult hindgut (5,46,47,115,125). It is noteworthy that all three…”

Section: Enteric Neuronsmentioning

confidence: 99%

The Digestive Tract of Drosophila melanogaster

2013

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The digestive tract plays a central role in the digestion and absorption of nutrients. Far from being a passive tube, it provides the first line of defense against pathogens and maintains energy homeostasis by exchanging neuronal and endocrine signals with other organs. Historically neglected, the gut of the fruit fly Drosophila melanogaster has recently come to the forefront of Drosophila research. Areas as diverse as stem cell biology, neurobiology, metabolism, and immunity are benefitting from the ability to study the genetics of development, growth regulation, and physiology in the same organ. In this review, we summarize our knowledge of the Drosophila digestive tract, with an emphasis on the adult midgut and its functional underpinnings. 377

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Insect ion transport peptides are derived from alternatively spliced genes and differentially expressed in the central and peripheral nervous system

Cited by 69 publications

References 92 publications

Identification and Characterization of Receptors for Ion Transport Peptide (ITP) and ITP-like (ITPL) in the Silkworm Bombyx mori

Identification and Characterization of Receptors for Ion Transport Peptide (ITP) and ITP-like (ITPL) in the Silkworm Bombyx mori

Neuropeptide F immunoreactive clock neurons modify evening locomotor activity and free‐running period in Drosophila melanogaster

The Digestive Tract of Drosophila melanogaster

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