Protein kinase A dependent and independent activation of the V-ATPase in Malpighian tubules of<i>Aedes aegypti</i>.

Tiburcy, Felix; Beyenbach, Klaus W.; Wieczorek, Helmut

doi:10.1242/jeb.078360

Cited by 28 publications

(43 citation statements)

References 65 publications

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“…1). Recent work in mosquito tubules has demonstrated that cAMP stimulates both V-ATPase activity and assembly of the holoenzyme (Tiburcy et al, 2013), which supports the findings in other insects [e.g. the blowfly (Baumann and Bauer, 2013)] and in other systems (Bond and Forgac, 2008).…”

Section: Cyclic Nucleotide Signallingsupporting

confidence: 77%

Cell signalling mechanisms for insect stress tolerance

Davies

Cabrero

Overend

et al. 2014

Journal of Experimental Biology

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Insects successfully occupy most environmental niches and this success depends on surviving a broad range of environmental stressors including temperature, desiccation, xenobiotic, osmotic and infection stress. Epithelial tissues play key roles as barriers between the external and internal environments and therefore maintain homeostasis and organismal tolerance to multiple stressors. As such, the crucial role of epithelia in organismal stress tolerance cannot be underestimated. At a molecular level, multiple cell-specific signalling pathways including cyclic cAMP, cyclic cGMP and calcium modulate tissue, and hence, organismal responses to stress. Thus, epithelial cell-specific signal transduction can be usefully studied to determine the molecular mechanisms of organismal stress tolerance in vivo. This review will explore cell signalling modulation of stress tolerance in insects by focusing on cell signalling in a fluid transporting epithelium -the Malpighian tubule. Manipulation of specific genes and signalling pathways in only defined tubule cell types can influence the survival outcome in response to multiple environmental stressors including desiccation, immune, salt (ionic) and oxidative stress, suggesting that studies in the genetic model Drosophila melanogaster may reveal novel pathways required for stress tolerance.

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Section: Cyclic Nucleotide Signallingsupporting

confidence: 77%

Cell signalling mechanisms for insect stress tolerance

Davies

Cabrero

Overend

et al. 2014

Journal of Experimental Biology

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“…H-89 inhibits the phosphorylation mechanisms of activated PKA and several other kinases (Lochner and Moolman, 2006); it has been widely used in insect studies to inhibit PKA (e.g., Beyenbach et al, 2009; Bhattacharya et al, 1999; Fechner et al, 2013; P. Gioino et al, 2014; Paluzzi et al, 2013; Tiburcy et al, 2013). Rp-cAMPS is an analog of cAMP that occupies the cAMP-binding sites of PKA, thereby preventing its activation (Lochner and Moolman, 2006).…”

Section: Methodsmentioning

confidence: 99%

Physiological characterization and regulation of the contractile properties of the mosquito ventral diverticulum (crop)

Calkins

DeLaat

Piermarini

2017

Journal of Insect Physiology

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In adult dipteran insects (flies), the crop is a diverticulum of the esophagus that serves as a food storage organ. The crop pumps stored contents into the alimentary canal for digestion and absorption. The pumping is mediated by peristaltic contractions of the crop musculature. In adult female mosquitoes, the crop (ventral diverticulum) selectively stores sugar solutions (e.g., nectar); proteinaceous blood meals by-pass the crop and are transferred directly to the midgut for digestion. The mechanisms that regulate crop contractions have never been investigated in mosquitoes. Here we provide the first physiological characterization of the contractile properties of the mosquito crop and explore the mechanisms that regulate crop contractions. Using an in vitro bioassay we found that the isolated crop spontaneously contracts in Ringer solution for at least 1 hour and its contractions are dependent on extracellular Ca2+. Adding serotonin (5- hydroxytryptamine, 5-HT) or a membrane-permeable analog of cyclic adenosine monophosphate (cAMP) to the extracellular bath increased the frequency of crop contractions. On the other hand, adding benzethonium chloride (BzCl; a chemical that mimics the effects of myosuppressins), H-89 or Rp-cAMPS (inhibitors of protein kinase A, PKA), or carbenoxolone (an inhibitor of gap junctions) reduced the frequency of the unstimulated, spontaneous and/or 5-HT-stimulated crop contractions. Adding aedeskinin III did not detectably alter crop contraction rates. In addition to pharmacological evidence of gap junctions, we demonstrated that the crop expressed several mRNAs encoding gap junctional proteins (i.e. innexins). Furthermore, we localized immunoreactivity for innexin 2 and innexin 3 to muscle and epithelial cells of the crop, respectively. Our results 1) suggest that 5-HT and myosupressins oppositely regulate contractile activity of the mosquito crop, and 2) provide the first evidence for putative roles of cAMP, PKA, and gap junctions in modulating contractile activity of the dipteran crop.

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“…In insect cells, assembly of the V-ATPase is under control of PKA, although PKA-independent assembly has also been demonstrated [45]. Upon maturation of dendritic cells, increased assembly of the V-ATPase on lysosomes promotes the low pH required for antigen processing [46].…”

Section: Regulation Of V-atpase Activitymentioning

confidence: 99%

Recent Insights into the Structure, Regulation, and Function of the V-ATPases

Cotter

Stransky

McGuire

et al. 2015

Trends in Biochemical Sciences

252

274

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The V-ATPases are ATP-dependent proton pumps that acidify intracellular compartments and are also present at the plasma membrane. They function in such processes as membrane traffic, protein degradation, viral and toxin entry, bone resorption, pH homeostasis and tumor cell invasion. V-ATPases are large, multi-subunit complexes composed of an ATP-hydrolytic domain (V1) and proton translocation domain (V0) and operate by a rotary mechanism. This review focuses on recent insights into their structure and mechanism, the mechanisms that regulate V-ATPase activity (particularly regulated assembly and trafficking) and the role of V-ATPases in such processes as cell signaling and cancer. These developments have highlighted the potential of V-ATPases as a therapeutic target for a variety of human diseases.

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Protein kinase A dependent and independent activation of the V-ATPase in Malpighian tubules ofAedes aegypti.

Abstract: SUMMARY Transepithelial ion transport in insect Malpighian

Cited by 28 publications

References 65 publications

Cell signalling mechanisms for insect stress tolerance

Cell signalling mechanisms for insect stress tolerance

Physiological characterization and regulation of the contractile properties of the mosquito ventral diverticulum (crop)

Recent Insights into the Structure, Regulation, and Function of the V-ATPases

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