The measurement of sperm motility and factors affecting sperm quality in cultured fish

Rurangwa, E.; Kime, David E.; Ollevier, Frans; Nash, J.P

doi:10.1016/j.aquaculture.2003.12.006

Cited by 569 publications

(513 citation statements)

References 147 publications

Supporting

Mentioning

422

Contrasting

Unclassified

Order By: Relevance

“…However, ideal dosages may vary according to the quality of the gametes (Bobe & Labbe, 2010), and the procedure employed to achieve fertilization (Rurangwa et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…However, more studies about gamete characterization and reproductive management during the process of artificial fertilization, as well as about the reproductive biotechniques in captivity are necessary for the preservation of this species. They may contribute directly to the rational use of gametes, increasing the success of the artificial propagation, like the application of effective inseminating doses (Rurangwa et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Inseminating dose and water volume applied to the artificial fertilization of Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae): Brazilian endangered fish

et al. 2016

View full text Add to dashboard Cite

The Steindachneridion parahybae is an endangered catfish from Brazil and strategies applied for gametes optimization are necessary. The aim of this study was to assess inseminating doses and water volume upon the fertilization, hatching rates and percentage of normal larvae in S. parahybae. Was used a randomized design in factorial scheme (4×4) with four inseminating doses: 1.0×10 4 , 1.0×10 5 , 1.0×10 6 , 1.0×10 7 spermatozoa oocyte -1 and four volumes of water: 1, 35, 65 and 95mL of water g -1 of oocytes. The combination of doses and volumes were performed in triplicates (n=48). Each incubator (1.5L of useful volume) with 1g of oocytes was considered as an experimental unit. Significant interaction between inseminating doses and volumes of water to the values of the fertilization rates and quadratic effect of doses and volume for the values of hatching rates were observed. The doses and volumes did not influence the percentage of normal larvae (87.70±5.06%). It is recommended the use of 5.5×10 6 spermatozoa oocyte -1 and 1mL of water g -1 of oocytes during in vitro fertilization procedure. These results allowed us to develop new biotechnological strategies applied to the conservation of S. parahybae.O Steindachneridion parahybae é um bagre ameaçado de extinção no Brasil e estratégias aplicadas para a otimização de gametas são necessárias. O objetivo deste estudo foi avaliar doses inseminantes e volume de água sobre os valores das taxas de fertilização, eclosão e larvas normais em S. parahybae. Utilizando-se um delineamento experimental casualizado em esquema fatorial (4×4), com quatro doses inseminantes: 1,0×10 4 ; 1,0×10 5 ; 1,0×10 6 ; 1,0×10 7 espermatozóides ovócito -1 e quatro volumes de água: 1; 35; 65 e 95mL de água g -1 de ovócitos. As combinações de doses e volumes foram realizadas em triplicatas (n=48). Cada incubadora (1,5L de volume útil) contendo 1g de ovócitos foi considerada como uma unidade experimental. Interações significativas entre doses inseminantes e volumes de água para os valores das taxas de fertilização e efeito quadrático das doses e do volume para os valores das taxas de eclosão foram verificadas. As dosagens e os volumes aplicados não influenciaram no percentual de larvas normais (87,70±5,06). Recomenda-se a aplicação de 5,5×10 6 espermatozoides ovócito -1 e a utilização de 1mL de água.g -1 de ovócitos no procedimento de fertilização artificial in vitro. Estes resultados permitiram desenvolver novas estratégias biotecnológicas aplicadas na conservação do S. parahybae.

show abstract

“…However, ideal dosages may vary according to the quality of the gametes (Bobe & Labbe, 2010), and the procedure employed to achieve fertilization (Rurangwa et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inseminating dose and water volume applied to the artificial fertilization of Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae): Brazilian endangered fish

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The spermatocrit is defined as the ratio of white packed material volume to the total volume of semen × 100 (Rurangwa et al, 2004). Microhaematocrit capillary tubes (75 mm in length and 1.1-1.2 mm in diameter) were filled with semen and one end of each tube was sealed with clay.…”

Section: Spermatocrit Methodsmentioning

confidence: 99%

“…A two-step dilution was used for motility activation according to the method suggested by Billard and Cosson (1992) for salmonid fish. Firstly, the semen was prediluted to a saline solution (composed of 4.68 g NaCl, 2.98 g KCl, 0.11 g CaCl 2 , 3.15 g Tris-HCl per one litre of distilled water (final pH = 9) at a ratio of 1/100 and secondly, the prediluted semen was subjected to a second dilution in a physiological serum (0.7% of NaCl) at a ratio of 1/20 and immediately 1 µl of solution was placed on the microscope stage and motility was analyzed by a semi-quantitative method (Rurangwa et al, 2004). In this regard, the motility was recorded on a videotape in the light microscopic field from about 5-7 s after the onset of motility until at least 90% of cells were immotile.…”

Section: Sperm Motilitymentioning

confidence: 99%

Evaluation of semen quality of endangered Caspian brown trout (Salmo trutta caspius) in different times of spermiation during the spawning season

Hajirezaee¹,

Amiri²,

Mirvaghefi³

et al. 2010

CZECH J ANIM SCI

View full text Add to dashboard Cite

Sperm motility and sperm production as well as organic and inorganic components present in the seminal plasma were measured at different time periods of spermiation during the spawning season in order to evaluate the semen quality in Caspian brown trout, Salmo trutta caspius. For such evaluation, males were divided into four groups based on the date of spermiation: group A (pre-mature broodstocks), group B and C (mid-mature broodstocks), group D (late-mature broodstocks). Our results showed that the Caspian brown trout produces semen of variable quality depending on the date in the period of spermiation. In this regard, the percentage of motile spermatozoa, duration of motility, sperm density, osmolality and also the concentrations of Na + , Cl -, K + , Ca 2+ , Mg 2+ and total protein content were significantly higher in mid-mature broodstocks (which matured in the middle of spawning season, i.e. groups B and C) compared to pre-mature (group A) and late-mature (group D) samples. Statistically significant positive correlations were also found between these semen parameters: sperm production vs Na

show abstract

“…The beneficial effect with rapid cooling may also rely on the use of dMSO as the cryoprotectant because dMSO can penetrate sperm cells rapidly and prolonged equilibration during slow cooling can exert more cytotoxicity of dMSO on sperm cells (he et al 2011) Although sperm motility rate is the key parameter in sperm quality, the cryopreservation process should also be evaluated based on the fertilization capacity of oocytes. However, the sperm:oocyte ratio and contact time may affect fertilization rates, hindering comparisons between different studies (Rurangwa et al 2004). To ensure maximum fertilization, sperm cells in excess are usually used for the fertilization of fish oocytes with cryopreserved sperm (viveiros et al 2009).…”

Section: -1 (Vuthiphandchai Et Al 2009) the Variation Inmentioning

confidence: 99%

Cryopreservation of mutton snapper ( Lutjanus analis) sperm

Sanches

Oliveira

Serralheiro

et al. 2013

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

This study aimed to develop a protocol of semen cryopreservation of the mutton snapper Lutjanus analis. The interaction between three extenders (pH 6.1; 7.8 and 8.2), two concentrations of dimethyl sulfoxide (dMSO, 5 and 10%) and three cooling rates (-90; -60 and -30°C.min ) on the sperm motility rate and motility time were analyzed by a factorial experiment. A sample of 30 fishes (1,261 ± 449 g) collected in the nature was kept in floating net cages. The semen was frozen by using cryogenic straws, in nitrogen vapour and transferred, later, to liquid nitrogen. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The highest sperm motility rate and motility time (P < 0.05) was achieved by combining extender C (ph 8.2) with dMSO (10%) and cooling rate of -60°C.min -1 (P < 0.05). The use of cryopreserved sperm presented fertilization rates higher than 59% validating the present protocol for mutton snapper.

show abstract

The measurement of sperm motility and factors affecting sperm quality in cultured fish

Cited by 569 publications

References 147 publications

Inseminating dose and water volume applied to the artificial fertilization of Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae): Brazilian endangered fish

Inseminating dose and water volume applied to the artificial fertilization of Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae): Brazilian endangered fish

Evaluation of semen quality of endangered Caspian brown trout (Salmo trutta caspius) in different times of spermiation during the spawning season

Cryopreservation of mutton snapper ( Lutjanus analis) sperm

Contact Info

Product

Resources

About