Inseminating dose and water volume applied to the artificial fertilization of Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae): Brazilian endangered fish

Sanches, Eduardo Antônio; Caneppele, Danilo; Okawara, Renan Yoshiharu; Damasceno, Danielle Zanerato; Bombardelli, Robie Allan; Romagosa, Elizabeth

doi:10.1590/1982-0224-20140158

Cited by 13 publications

(9 citation statements)

References 30 publications

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“…In this study we observed that CPE and mGnRHa + MET treatments provoked ovulation in L. friderici, but the lower CPE dose treatment was the unique that provided viable embryos. This pattern of response is unusual mainly because conventional CPE dose (0.5 mg and 5.5 mg/kg, with 12h interval, with some variations) has provided ovulation and viable embryos in many fish species evaluated worldwide (Mylonas et al, 2010) and is the mainly hormonal therapy used for inducing tropical migratory fish ovulation (Caneppele et al, 2015;Souza et al, 2015;Ittzés et al, 2015;Viveiros et al, 2015;Sanches et al, 2016;Pereira et al, 2017;García et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Wild Leporinus friderici induced spawning with different dose of mGnRHa and metoclopramide or carp pituitary extract

Souza

Kuradomi

Rodrigues³

2020

Anim Reprod

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How to cite: Souza TG, Kuradomi RY, Rodrigues SM, Batlouni SR. Wild Leporinus friderici induced spawning with different dose of mGnRHa and metoclopramide or carp pituitary extract. Anim Reprod. 2020;17(1):e20190078. AbstractBreeding technology is of utmost importance for reproduction of wild fish in captivity for the reintroduction and selective breeding programs purposes. The main challenge is that when applied to wild undomesticated specimens, conventional protocols often cause breeders and/or embryo mortality and spawning failure. In this study, we evaluated the reproductive performance of wild Leporinus friderici, a great importance fish for subsistence fishing in South American rivers, applying conventional and lower-dose hormonal therapies by means of two consecutive experiments. In the first, a conventional (0.5 and 5.5 mg/kg) and a lower carp pituitary extract (CPE) dose (0.5 and 1.0 mg/kg) were applied. In the second, a conventional mammalian GnRH analogue associated with metoclopramide (mGnRHa + MET) (40 µg mGnRHa + 20 mg MET/kg) and a lower dose (4 μg mGnRHa + 2 mg MET/kg and 8 µg + 4 mg of mGnRHa + MET/kg) were applied. Ovulation was observed in all treatments, however, only lower CPE protocol provided viable embryos. High levels of 17α,20β-dihydroxy-4-pregnen-3-one (DHP) and 17β estradiol (E 2 ) detected in conventional, but not in lower CPE dose, at ovulation, might be associated to the mortality of the embryos. The use of lower CPE dose applied here was the best way to obtain L. friderici viable embryos. These results directly contribute to the knowledge about poorly explored effects of reproductive management and hormonal therapies in wild-type breeders, showing that the use of reduced doses may be an alternative to reproductive success.

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Section: Discussionmentioning

confidence: 99%

Wild Leporinus friderici induced spawning with different dose of mGnRHa and metoclopramide or carp pituitary extract

Souza

Kuradomi

Rodrigues³

2020

Anim Reprod

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“…The environment used for in vitro fertilization has been shown to affect the spz egg −1 ratio. For example, the volume or space provided for fertilization affected the spz egg −1 ratio, as larger volumes increased the space to be travelled to fertilize the egg and increased the number of spz required [ 28 , 55 , 56 ]. Therefore, variation in the fertilization environment complicates the comparison of different studies within and among species.…”

Section: Discussionmentioning

confidence: 99%

Low sperm to egg ratio required for successful in vitro fertilization in a pair-spawning teleost, Senegalese sole ( Solea senegalensis )

et al. 2021

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Cultured Senegalese sole ( Solea senegalensis ) breeders fail to spawn fertilized eggs. The implantation of large-scale in vitro fertilization protocols, to solve this problem, has been frustrated by low production of poor quality sperm. Cultured females were induced to ovulate with a 5 µg kg −1 single injection of gonadotropin-releasing hormone agonist (GnRHa) and viable eggs (82.6 ± 9.2% fertilization) were stripped 41:57 ± 1:46 h after the injection. Sperm was collected from cultured males, diluted in modified Leibovitz and used fresh to fertilize the eggs. Males were not treated with hormones. A nonlinear regression, an exponential rise to a maximum ( R = 0.93, p < 0.0001) described the number of motile spermatozoa required to fertilize a viable egg and 1617 motile spermatozoa were sufficient to fertilize 99 ± 12% (±95% CI) of viable eggs. Similar, spermatozoa egg −1 ratios of 592 ± 611 motile spermatozoa egg −1 were used in large-scale in vitro fertilizations (190 512 ± 38 471 eggs). The sperm from a single male (145 ± 50 µl or 8.0 ± 6.8 × 10 8 spermatozoa) was used to fertilize the eggs. The mean hatching rate was 70 ± 14% to provide 131 540 ± 34 448 larvae per fertilization. The viability of unfertilized eggs stored at room temperature decreased gradually, and the sooner eggs were fertilized after stripping, the higher the viability of the eggs. The collection of sperm directly into a syringe containing modified Leibovitz significantly increased the percentage of motile spermatozoa (33.4 ± 12.2%) compared with other collection methods. The spz egg −1 ratios for Senegalese sole were at the lower end of ratios required for fish. Senegalese sole have a pair-spawning reproductive behaviour characterized by gamete fertilization in close proximity with no sperm competition. The provision of a large-scale in vitro fertilization protocol (200 µl of sperm per 100 ml of eggs) will enable the industry to operate sustainably and implement breeding programmes to improve production.

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“…The diameter directly influences the ideal proportion of sperm:oocyte because the distance to be covered by the sperm influences the fertilization capacity. Shimoda et al (2007) and Suquet et al (1995) reported that the larger the diameter, the more sperm cells are needed to fertilize the oocytes, and the smaller the diameter of the oocyte, the greater the likelihood of spermatozoa reaching the micropyle and fertilizing it.…”

Section: Discussionmentioning

confidence: 99%

“…Studies related to the ideal number of sperm per oocyte and/or volume of the activating solution have been applied to several species of freshwater teleosts, such as Rhamdia quelen (Bombardelli et al, 2006;Adames et al, 2015), Rhinelepis aspera (Sanches et al, 2009), Brycon orbignyanus (Felizardo et al, 2010), Piaractus mesopotamicus (Sanches et al, 2011), B. insignis (Shimoda et al, 2007), Salminus brasiliensis and Colossoma macropomum (Leite et al, 2013), P. magdalenae (García et al, 2015), and Steindachneridion parahybae (Sanches et al, 2016). However, for P. brevis there are no reports in the literature on such reproductive aspects.…”

Section: Introductionmentioning

confidence: 99%

Determination of the ideal volume of activating solution and the optimal ratio of spermatozoa per oocyte for Prochilodus brevis fertilization

Oliveira-Araújo

Lopes

Nunes

et al. 2019

Zygote

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SummaryProchilodus brevis is a rheophilic species with a threatened natural population that promotes studies aimed at optimizing reproduction in captivity. The correct quantity of inseminating dose and activating solution volume significantly improves fertilization rates, thereby increasing productivity in captivity. The objective of this study was to determine the proportion of sperm per oocyte and the ideal volume of activating solution to be used in the assisted fertilization of P. brevis. Gametes were collected and fertilization performed in two steps. In step 1, the ideal proportion of spermatozoa was determined based on the fertilization rate:oocyte by testing six doses of semen: D1 = 30 × 103, D2 = 150 × 103, D3 = 300 × 103, D4 = 3 × 106, D5 = 5 × 106, and D6 = 10 × 106. In step 2, the fertilization and hatching rates were evaluated using different volumes of activating solution (V1 – 25 ml, V2 – 50 ml, V3 – 75 ml,V4 – 100 ml, V5 – 125 ml, and V6 – 150 ml). A linear regression equation was estimated from steps 1 and 2. The Student–Newman–Keuls test was used to compare the means. In step 1, the percentage of fertilization increased linearly, reaching a plateau of 51.69%. In step 2, the best fertilization rates were obtained with an estimated ideal volume of 75.64 ml per 2 ml of oocytes. Therefore, the proportion of 928,410.29 sperm:oocyte, associated with the volume of 75.64 ml of water per 2 ml of oocytes, provided the maximum reproductive performance for P. brevis.

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Inseminating dose and water volume applied to the artificial fertilization of Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae): Brazilian endangered fish

Cited by 13 publications

References 30 publications

Wild Leporinus friderici induced spawning with different dose of mGnRHa and metoclopramide or carp pituitary extract

Wild Leporinus friderici induced spawning with different dose of mGnRHa and metoclopramide or carp pituitary extract

Low sperm to egg ratio required for successful in vitro fertilization in a pair-spawning teleost, Senegalese sole ( Solea senegalensis )

Determination of the ideal volume of activating solution and the optimal ratio of spermatozoa per oocyte for Prochilodus brevis fertilization

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