The major histocompatibility complex of the rat,RT1

Götze, Dietrich

doi:10.1007/bf01844039

Immunogenetics

1978

DOI: 10.1007/bf01844039

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The major histocompatibility complex of the rat,RT1

Dietrich Götze

Abstract: The antigenic determinants expressed on RBC and lymphocytes and coded for by the MHC, RT1,of the MNR (RT1 ( m )) rat strain were compared to those of the BN.DA(RT1 ( a )), ALB (RT1 ( b )), and AUG (RT1 ( c )) strains by direct cytotoxicity and absorption analysis with RT1 typing sera, sera produced against MNR cells, and sera produced in MNR responders against cells carrying thea, b, andc haplotype determinants. The results indicate that MNR shares major class I (A) antigens with DA, and major class II (B) det… Show more

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1985

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Cited by 9 publications

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“…In particular, the RT1P and RTI"' haplotypes exhibited grossly dissimilar restriction patterns despite their apparent coinheritance of at least one RT1 .A allele (29,30). Of greater interest is the dissimilarity of restriction patterns associated with the RTI1 and RTl l haplotypes of LEW and F344, respectively.…”

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confidence: 98%

“…Class I genes encode approximately 45,000-dalton cell surface glycoproteins; in the case of the mouse MHC, H-2, approximately 30 class I sequences have been identified by gene cloning experiments (2). Among these genes are the previously identified K, D, and L (3) genes expressed on the majority of somatic cells and the Qa (4) and Ti genes (5) differentially expressed on lymphocyte subpopulations.…”

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Evidence for extensive polymorphism of class I genes in the rat major histocompatibility complex (RT1).

Palmer¹,

Wettstein²,

Frelinger³

1983

Proc. Natl. Acad. Sci. U.S.A.

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The major histocompatibility complex of the rat (RT1) has been poorly characterized with respect to the number, linkage, and polymorphism of class I genes. To estimate the number of class I RT1 genes and the relative extent of their polymorphism, we performed Southern blot analysis with liver DNA from rat strains expressing eight RT1 haplotypes. After digestion with EcoRI and BamHI, the DNA was separated on agarose gels, blotted onto nitrocellulose, and hybridized with mouse H-2 cDNA probes, pH-211 and pH-2iHa. Ten to 20 EcoRI and 13 to 20 BamHI bands hybridized with pH-2I11 and pH-211a; restriction fragment length patterns were observed to be highly polymorphic. The restriction fragments associated with different RT1 haplotypes differed by 17-70%; this range is similar to the differences observed between mouse H-2 haplotypes. The same restriction fragment pattern was observed in DNA from three different rat strains sharing the same RTI allele, confirming that the patterns were RTI-associated. Further, the RTI1 and RT11"' haplotypes, which differ at a single previously identified RT1-linked locus, were associated with EcoRI restriction pattern differences of 39-50%, confirming the supposition that RT1 class I genes identified by previous serological and T-cell-mediated assays have identified only a minority of the actual number of RT1-linked class I genes. In summary, the results reported in this communication demonstrate that the RT1 complex encompasses a large family of highly polymorphic class I genes similar to the H-2 and HL-A complexes of mouse and man.The major histocompatibility complexes (MHCs) of mammals include polymorphic class I and class II genes (1), whose products regulate various aspects of the immune response. Class I genes encode approximately 45,000-dalton cell surface glycoproteins; in the case of the mouse MHC, H-2, approximately 30 class I sequences have been identified by gene cloning experiments (2). Among these genes are the previously identified K, D, and L (3) genes expressed on the majority of somatic cells and the Qa (4) and Ti genes (5) differentially expressed on lymphocyte subpopulations. Class II genes encode Ia molecules composed of two glycoprotein subunits with molecular weights of approximately 32,000 and 28,000 (6); class II molecules are primarily expressed on B cells (7) and macrophages (8).The outstanding characteristic of class I and class II MHC genes of the two most extensively studied species, mouse and man (HL-A), is their high level of polymorphism. In the mouse, approximately 50 different alleles at both the H-2K and H-2D loci have been identified in inbred and wild mice (9, 10). The rat MHC, RTJ, has been suggested to be significantly less polymorphic than the H-2 and HL-A complexes. The RTI complex is composed of (i) class I genes, which are RTJ.A (H-2K and H-2D analogs) (11, 12), RTJ.C (Qa analog) (13) The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in acco...

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confidence: 98%

mentioning

confidence: 99%

Evidence for extensive polymorphism of class I genes in the rat major histocompatibility complex (RT1).

Palmer¹,

Wettstein²,

Frelinger³

1983

Proc. Natl. Acad. Sci. U.S.A.

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Serological characterization of rat class II (RT1.B) alloantigens analysis of the RT 1 ,RT1 u , and RT1 n haplotypes

Wettstein

1981

Immunogenetics

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Class I and class II restriction pattern polymorphisms associated with independently derived RT1 haplotypes in inbred rats

1985

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This communication reports the DNA level identification of class I and class II sequences associated with 20 RT1 haplotypes which have been assigned previously to eight RT1 groups. Sixteen to 22 bands in genomic blots hybridized with the mouse pH-2III class I cDNA probe. Only the three RT1k haplotypes associated with identical class I restriction fragment patterns. Differences in restriction bands between putatively identical RT1 haplotypes were either less than or equal to 6%, or greater than 50%, suggesting a relatively high level of recombination between serologically identified RT1.A genes and the majority of class I sequences. Restriction fragment patterns associated with three RT1u haplotypes differed by less than 6%. However, intra-RT1a, intra-RT1b, and intra-RT1l restriction fragment differences were between 50 and 64%. In specific cases, different RT1 haplotypes associated with identical class I restriction patterns, e.g., RT1m (MNR) and RT1d (MR); higher resolution confirmed the difference (two bands) between RT1m and RT1d. Results of hybridization with the human DC1 beta probe confirmed that the AVN RT1a and NSD RT1b haplotypes were generated by recombinations within the vicinity of the RT1.B:RT1.D regions. These results demonstrate that a previous classification of RT1 haplotypes was incomplete and did not include the majority of class I and class II sequences which distinguish RT1 haplotypes.

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The major histocompatibility complex of the rat,RT1

Cited by 9 publications

References 22 publications

Evidence for extensive polymorphism of class I genes in the rat major histocompatibility complex (RT1).

Evidence for extensive polymorphism of class I genes in the rat major histocompatibility complex (RT1).

Serological characterization of rat class II (RT1.B) alloantigens analysis of the RT 1 ,RT1 u , and RT1 n haplotypes

Class I and class II restriction pattern polymorphisms associated with independently derived RT1 haplotypes in inbred rats

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