Serological characterization of rat class II (RT1.B) alloantigens analysis of the RT 1 ,RT1 u , and RT1 n haplotypes

Wettstein, Peter J.

doi:10.1007/bf00350126

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1985

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(2 citation statements)

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“…The observed cross-reactivity of antibody 1-5A4 with gene products of the c haplotype seems reasonable, since two-dimensional gels showed that rats of the haplotype a and c carry very similar class II antigens [7,37]. In addition, the antigenic specificity 6 (RTl.B) is the only one shared by the a and c haplotype [40].…”

Section: Discussionmentioning

confidence: 99%

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Studies of Monoclonal Antibodies Specific for Major Histocompatibility Complex Products of the Rat

et al. 1985

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Sixteen monoclonal antibodies against antigens coded for by the RT1 complex of the rat have been produced. Fourteen arc specific for the a haplotype: six recognize class II and eight class I antigens. Two are specific for the I haplotype, one reacting with class I and the other with class II antigens. By means of these monoclonal antibodies four independent clusters of antigens for class I antigens of the a haplotype and three for class II antigens could be defined. The three antigenic sites of class II antigens reside on the same heterodimer. The monoclonals described here are characterized with regard to Ig class and subclass, pI, and complement‐activating capacity.

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Section: Discussionmentioning

confidence: 99%

“…Cells were washed once in phosphate-buffered saline (PBS),Ca" -and Mg' -free. Thymocyieswere resus-[lended in PBS and adjusted to between 40 and Sll x l()"cellsjml-Erythrocytes trom spleens were lysed by hypotonic shock, using (I,M midistilledwatci tor 2 sec. followed by II.…”

Section: Methodsmentioning

confidence: 99%