Class I and class II restriction pattern polymorphisms associated with independently derived RT1 haplotypes in inbred rats

Wettstein, Peter J.; Faas, Susan J.; Buck, David

doi:10.1007/bf00430590

Cited by 9 publications

(1 citation statement)

References 37 publications

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“…Serological techniques have failed to demonstrate extensive MHC class I polymorphism in the rat (26). However, polymorphism has been detected at the rat MHC class I loci by RFLP analysis (27 (28) but may be monomorphic at its class I loci (29). An analysis of the hamster MHC class I-encoding genes by using RFLP showed limited polymorphism.…”

Section: Discussionmentioning

confidence: 99%

A primate species with limited major histocompatibility complex class I polymorphism.

Watkins

Hodi

Letvin

1988

Proc. Natl. Acad. Sci. U.S.A.

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Extensive polymorphism at the major histocompatibility complex (MHC) is thought to confer immune protection on populations. A New World primate, the cottontop tamarin (Saguinis oedipus), has a high prevalence of ulcerative colitis and adenocarcinoma of the colon and dies after infection with several human viruses. Lymphocytes from all animals tested expressed one common MHC class I allelic product. Another MHC ciass I allelic product was expressed by 39 of 41 tested animals. Four other ailelic products were also expressed on the lymphocytes of these animals at a frequency of >50%. MHC class II gene products, however, were polymorphic. Restriction fragment length polymorphism analysis confirmed that there were a limited number of cotton-top tamarin MHC class I alleles, whereas the MHC class II gene loci were polymorphic. This sharing of MHC class I alleles is unprecedented in a higher primate species and may play a role in the susceptibility of this endangered species to pathogens.Major histocompatibility complex (MHC) glycoproteins play an important role in immune cell interactions. Codominant expression of class I and class II allelic products and an extraordinary degree of polymorphism of these products in a species are thought to confer a survival advantage to individuals and to the species by facilitating the ability of the immune system to interact with a variety of pathogens.Cotton-top tamarins (Saguinus oedipus) are highly susceptible to a variety of pathogenic processes. As many as 60% of captive S. oedipus spontaneously develop ulcerative colitis and 15% of these animals develop adenocarcinoma of the colon (1). S. oedipus also develops fatal lymphoproliferative syndromes after infection with Epstein-Barr virus and Herpesvirus saimiri (2, 3). Additionally, these tamarins are highly susceptible to retrovirus-induced sarcomas and fatal measle virus infections (3). Interestingly, cotton-top tamarins are usually born as dizygotic twins and an anastamosing placental circulation results in their development as naturally occurring bone-marrow-chimeric animals (4).In this study, we demonstrate that the cotton-top tamarin possesses very few allelic products at its three MHC class I loci. Its MHC, however, encodes for a diversity of class II allelic products, suggesting that its limited number of class I alleles are not due to a recent founder effect. This unprecedented limited MHC class I polymorphism in a primate species may play a role in the cotton-top tamarin's extreme susceptibility to pathogens. Celi Culture. Peripheral blood lymphocytes (PBLs) were separated from 2 to 10 ml of heparinized blood by using Dextran or Ficoll/diatrizoate gradient centrifugation. These cells were cultured at 106 cells per ml with phytohemagglutinin-P (Difco) at 1 pug/ml in RPMI 1640 medium (GIBCO) supplemented with heat-inactivated fetal bovine serum (Sterile Systems, Logan, UT) to a final concentration of 10% (vol/vol), 2 mM L-glutamine, 50 1LM 2-mercaptoethanol, 20 mM Hepes, gentamycin at 40 ,ug/ml, amphotericin B at 0...

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Section: Discussionmentioning

confidence: 99%

A primate species with limited major histocompatibility complex class I polymorphism.

Watkins

Hodi

Letvin

1988

Proc. Natl. Acad. Sci. U.S.A.

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Molecular characterization and genetic mapping of class I and class II MHC genes of the domestic cat

Yuhki

O’Brien

1988

Immunogenetics

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The major histocompatibility complex (MHC) of the domestic cat has been poorly characterized to date, primarily because of numerous difficulties in the preparation of allotypic sera. We present here a comparative analysis of class I and class II genes in domestic cat populations using molecular probes of the MHC from man and mouse. The cat possesses a minimum of 20 class I loci and 5 class II genes per haploid genome. Class I genes of the domestic cat expressed limited restriction fragment length polymorphism. The average percent difference of the size of DNA fragments between individual cats was 9.0%, a value five times lower than the value for mice, but comparable to the human DNA polymorphism level. Class I and class II genes were both genetically mapped to feline chromosome B2 using a panel of rodent x cat somatic cell hybrids. Since feline chromosome B2 is syntenically homologous to human chromosome 6 and mouse chromosome 17, these results affirm the linkage conservation of the MHC-containing linkage group in the three mammalian orders.

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The major histocompatibility complex of tassel-eared squirrels

Wettstein

States

1986

Immunogenetics

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The complexity and polymorphism of sequences related to the class I and class II genes of mammalian major histocompatibility complexes (MHCs) were investigated in the tassel-eared squirrel subspecies Sciurus aberti kaibabensis or Kaibab squirrel. Kaibab squirrels are geographically isolated on the Kaibab plateau north of the Grand Canyon in Arizona. Genomic DNA from 22 individuals was digested with Eco RI and Bam HI, electrophoresed, blotted, and hybridized with a panel of human class I and class II probes. Sequences homologous to DR alpha, DR beta, DQ beta probes were observed. A single, nonpolymorphic DR alpha-related sequence and multiple, polymorphic DQ alpha-related sequences were observed. Hybridization with DR beta and DQ beta probes revealed multiple, polymorphic sequences with such specificity that no bands were observed to hybridize with both probes. The level of polymorphism of beta sequences exceeded that observed with alpha sequences. Further, three Eco RI bands apparently included at least parts of both alpha and beta sequences. Hybridization of genomic blots with the HLA-B7 class I probe revealed a number of bands comparable in size range and number to other mammalian species. However, only a minor percentage of bands were observed to segregate. The inheritance of these five families of sequences appeared to be neither concordant nor random in the sample population. Based on prior conclusions in other species, these class I and class II sequences are presumed to map to the Kabib MHC, TLSA. Although DQ alpha- and DQ beta-related sequences were concordantly inherited, segregating sequences in the other families could not be assigned to identifiable, segregating haplotypes. These observations suggest that the present-day TSLA haplotypes have been derived from a limited number of progenitor haplotypes through repeated, intra-TSLA recombination.

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Class I and class II restriction pattern polymorphisms associated with independently derived RT1 haplotypes in inbred rats

Cited by 9 publications

References 37 publications

A primate species with limited major histocompatibility complex class I polymorphism.

A primate species with limited major histocompatibility complex class I polymorphism.

Molecular characterization and genetic mapping of class I and class II MHC genes of the domestic cat

The major histocompatibility complex of tassel-eared squirrels

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