Molecular characterization and genetic mapping of class I and class II MHC genes of the domestic cat

Yuhki, Naoya; O’Brien, Stephen J.

doi:10.1007/bf00364427

Cited by 37 publications

(19 citation statements)

References 40 publications

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“…However, it cannot be said whether the B 2 q deletion was terminal or interstitial. Furthermore, the exact band location of the MHC on the q arm is not known (Yuhki and O'Brien, 1988). showed the highest percentage of occurrence.…”

Section: Karyotypingmentioning

confidence: 99%

Chromosome abnormalities and oncogenesis in cat leukemias

Gulino

1992

Cancer Genetics and Cytogenetics

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Section: Karyotypingmentioning

confidence: 99%

Chromosome abnormalities and oncogenesis in cat leukemias

Gulino

1992

Cancer Genetics and Cytogenetics

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“…loci of most other mammalian species has been hampered by difficulties in raising serological reagents that define the cat MHC gene products (7, 13-16, 19, 20 (17)(18)(19) have described for the cat MHC, less than a third of the cats acutely rejecting three or more skin grafts produced detectable levels of lymphocytotoxic alloantibodies. In most species, the rejection of grafts is almost always followed by the appearance of lymphocytotoxic alloantibodies in the host's serum (7,32,33).…”

Section: Discussionmentioning

confidence: 99%

“…Early observations that cats show prolonged allograft survival (14)(15)(16), fail to develop lymphocytotoxic alloantibodies after pregnancy or transfusions (13), and succumb to multiple viral infections have led to speculation that cats may have limited or no polymorphism at the MHC (7,13). However, recent reports suggest that domestic cats do have polymorphic class I genes and MHC gene products (17)(18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of FLA, the cat major histocompatibility complex.

Winkler¹,

Schultz²,

Cevario³

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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The major histocompatibility complex (MHC) of the domestic cat (termed FLA) has been refractile to genetic and serological definition largely because of repeated failure to detect cytotoxic antibodies in multiparous cats or to elicit antibody following allogeneic lymphocyte immunization. We have developed a protocol for producing cytotoxic alloantisera in the cat following rejection of multiple surgical skin grafts. Of 59 cats subjected to grafting, 13 produced lymphocytotoxic antisera which had varying specificities among a panel of outbred cat cells. A population cluster analysis of the 13 alloantisera permitted the identification of six clusters of overlapping FLA specificities. The major histocompatibility complex (MHC) is a multigene cluster in mammals encoding two distinct classes of molecules involved in the presentation of foreign antigen to the T-cell receptor (1-5). Class I genes encode a 45-kDa glycoprotein that is noncovalently linked with an invariant light chain, P2-microglobulin (f82m), on the surfaces of virtually all nucleated cells (4, 6). Class II genes encode the heavy (32-35 kDa) and light (25-28 kDa) subunits of the noncovalently associated af3 heterodimeric glycoproteins expressed on the surfaces of antigen-presenting cells and activated T cells (5). By serological analysis, both class I and class II antigens are abundantly polymorphic in most outbred species. DNA restriction analysis has also revealed extensive polymorphism of the MHC of mice, humans, and several other species (7,8). The function of both class I and class II antigens is to present foreign, often viral, antigen to the T cell receptor. In this way, the cellular immune response is directed to parasitized cells instead of free virus particles (9,10).The domestic cat is an important model for at least two viral diseases of the immune system in humans, human T cell leukemia and acquired immunodeficiency syndrome (AIDS) (11,12). The characterization of the feline MHC as a prerequisite for the study of disease progression has been difficult because of an apparent inability to raise typing alloantisera in the cat (13 MATERIALS AND METHODSDetails of allograft transplantation are described elsewhere (ref. 21; C.W. and S.O., unpublished data). Briefly, split thickness skin grafts were surgically exchanged between selected cats at 21-day intervals. In some cases, intraperitoneal booster injections of peripheral blood lymphocytes (PBLs) from 15 ml of blood were performed at 4-day intervals after grafting (21). Serum was collected from graft cats weekly and used in a cat complement-dependent cytotoxicity assay. A modification of a two-stage assay using 51Cr release as an indicator ofcell death was used to detect alloantibodies against donor cat lymphocytes. The monoclonal antibody W6/32 (23), specific for human class I heavy chain, and the monoclonal antibodies IB5 (human DR and DQ specific) (24) and ISCR3 (mouse I-EK specific) (25) were used as positive controls in immunoprecipitation experiments.Interleukin 2-stimulated or fr...

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“…The feline MHC (FLA)-DRB gene also displays an extensive degree of genetic polymorphism [10,23,24]. Sixty-six FLA-DRB sequences have been found by PCRsequence-based typing in 45 cats [10,24], and those have been divided into 37 genes and 10 subgroups according to the RFLP pattern by us [10].…”

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confidence: 99%

Effects of Genotype Matching of Feline Major Histocompatibility Complex (FLA) Class II DRB on Skin-allograft Transplantation in Cats.

Kuwahara

Kitoh

Kobayashi

et al. 2001

The Journal of Veterinary Medical Science

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ABSTRACT. In order to confirm the effects of matching of expressed feline major histocompatibility complex (FLA) class II DRB genotype on transplantation immunity in cats, skin-allogeneic transplantation was carried out between cats, in which DRB genes expressed were genotyped by the RT-PCR-RFLP method using group-specific primers. Duration until grafts were rejected was 14.63 ± 1.69 days (mean ± standard deviation) in the pairs that had the same type of subgroups, 7.25 ± 0.71 days in the pairs that had one different type of subgroup and 6.88 ± 0.35 days in the pairs that had two different types of subgroups. The duration of graft survival in the pairs with the same type of subgroups was significantly longer (P<0.01) than those in the pairs with different types. Although FLA components involved in transplantation immunity should not only be DRB genes, it was suggested that the expressed FLA-DRB genotype might associate with feline transplantation immunity, and that typing and matching of expressed FLA-DRB genes might be one of the important factors in the control of feline transplantation immunity.

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Molecular characterization and genetic mapping of class I and class II MHC genes of the domestic cat

Cited by 37 publications

References 40 publications

Chromosome abnormalities and oncogenesis in cat leukemias

Chromosome abnormalities and oncogenesis in cat leukemias

Genetic characterization of FLA, the cat major histocompatibility complex.

Effects of Genotype Matching of Feline Major Histocompatibility Complex (FLA) Class II DRB on Skin-allograft Transplantation in Cats.

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