The long and short of non-coding RNAs during post-natal growth and differentiation of skeletal muscles: Focus on lncRNA and miRNAs

Butchart, Lauren C.; Fox, Archa H.; Shavlakadze, Tea; Grounds, Miranda D.

doi:10.1016/j.diff.2016.05.003

Cited by 36 publications

(32 citation statements)

References 77 publications

(89 reference statements)

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“…Mice were housed in a room of Nanjing Agricultural University under conventional conditions with appropriate temperature and humidity and fed a standard diet. Four mice at 8 wk of age were used to collect different tissues to measure the expression of AK017368 by quantitative PCR (qPCR) (22). Three-week-old mice were injected with 100 ml final volumes of small interfering (si)AK017368 or negative control (NC) oligos into the gastrocnemius muscles of the right and left hind legs, respectively.…”

Section: Animals and Cellsmentioning

confidence: 99%

“…The natural Argonaute protein forms ABBREVIATIONS: ceRNA, competing endogenous RNA; DM, differentiation medium; EdU, 5-ethynyl-29-deoxyuridine; GM, growth medium; H&E, hematoxylin and eosin; lincRNA, long intervening noncoding RNA; lncRNA, long noncoding RNA; miR, micro-RNA; MyHC, myosin heavy chain; MyoG, myogenin; NC, negative control; qPCR, quantitative PCR; siRNA, small interfering RNA; TNRC, trinucleotide repeat-containing; WT, wild type the core of RNA-induced silencing complex (19) and can cleave mRNA molecules (20). Therefore, a novel layer of regulation that integrates DNA, mRNA, lncRNA, and miRNA has been uncovered (21) and highlights the complexity of regulatory network in skeletal muscle myoblasts (22).…”

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confidence: 99%

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IncRNA AK017368 promotes proliferation and suppresses differentiation of myoblasts in skeletal muscle development by attenuating the function of miR‐30c

et al. 2017

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Long noncoding RNAs (lncRNAs) have been reported to play diverse roles in biologic and pathologic processes, including myogenesis. We found that lncRNA is highly expressed in skeletal muscle cells. Functional analyses showed that overexpression of promoted proliferation and restrained differentiation of myoblasts; whereas inhibition of had completely opposite effects In mice, knockdown of promoted muscle hypertrophy RNA molecules of acted mechanistically as competing endogenous RNAs to target micro-RNA (miR)-30c, which was supported by the results of bioinformatics analyses and dual-luciferase reporter assays. It has been shown that lncRNA competes with trinucleotide repeat containing-6A () for miR-30c. was previously reported to promote proliferation and inhibit differentiation of myoblast cells, whereas miR-30c targets the 3'-UTR of mRNA to weaken its function. Taken together, lncRNA promotes proliferation and inhibits differentiation of myoblast cells by attenuating function of miR-30c.-Liang, T., Zhou, B., Shi, L., Wang, H., Chu, Q., Xu, F., Li, Y., Chen, R., Shen, C., Schinckel, A. P. lncRNA promotes proliferation and suppresses differentiation of myoblasts in skeletal muscle development by attenuating the function of miR-30c.

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Section: Animals and Cellsmentioning

confidence: 99%

mentioning

confidence: 99%

IncRNA AK017368 promotes proliferation and suppresses differentiation of myoblasts in skeletal muscle development by attenuating the function of miR‐30c

et al. 2017

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“…This process causes enlarged myofibre volume as a result of an increase in both cross‐sectional area and length, and is concurrent with increased protein synthesis within myofibres leading to muscle growth or hypertrophy . During this period of hypertrophy, which, in mice, ends by 12 weeks of age, the sarcolemma dynamically and constantly expands, whereas, during adult life, it maintains almost the same size under normal healthy conditions . Investigation of myofibre sizes and sarcolemmal properties in actively growing and mature myofibres in homeostasis could have major implications for understanding the clinical onset of particular genetic muscle diseases, such as Duchenne muscular dystrophy and dysferlinopathies, where significant changes in size composition occur with disease progression.…”

Section: Introductionmentioning

confidence: 99%

The effects of ageing on mouse muscle microstructure: a comparative study of time‐dependent diffusion MRI and histological assessment

et al. 2018

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The investigation of age-related changes in muscle microstructure between developmental and healthy adult mice may help us to understand the clinical features of early-onset muscle diseases, such as Duchenne muscular dystrophy. We investigated the evolution of mouse hind-limb muscle microstructure using diffusion imaging of in vivo and in vitro samples from both actively growing and mature mice. Mean apparent diffusion coefficients (ADCs) of the gastrocnemius and tibialis anterior muscles were determined as a function of diffusion time (Δ), age (7.5, 22 and 44 weeks) and diffusion gradient direction, applied parallel or transverse to the principal axis of the muscle fibres. We investigated a wide range of diffusion times with the goal of probing a range of diffusion lengths characteristic of muscle microstructure. We compared the diffusion time-dependent ADC of hind-limb muscles with histology. ADC was found to vary as a function of diffusion time in muscles at all stages of maturation. Muscle water diffusivity was higher in younger (7.5 weeks) than in adult (22 and 44 weeks) mice, whereas no differences were observed between the older ages. In vitro data showed the same diffusivity pattern as in vivo data. The highlighted differences in diffusion properties between young and mature muscles suggested differences in underlying muscle microstructure, which were confirmed by histological assessment. In particular, although diffusion was more restricted in older muscle, muscle fibre size increased significantly from young to adult age. The extracellular space decreased with age by only~1%. This suggests that the observed diffusivity differences between young and adult muscles may be caused by increased membrane permeability in younger muscle associated with properties of the sarcolemma. KEYWORDS apparent diffusion coefficient, diffusion MRI, diffusion-weighted imaging, hind-limb muscles, histology, maturation, mouse, time-dependent diffusion MRI | INTRODUCTIONSkeletal muscle is the most abundant tissue in the human body, comprising 30-40% of the body weight, 1 and is the major site of metabolic activity. 2 Its function, which is strictly dependent on muscle cytoarchitecture, 3 enables vital actions, i.e. breathing, posture and locomotion, and affects systemic processes, such as homeostasis and the immune response.Skeletal muscle mass and fibre size vary according to physiological and pathological conditions, 4 which influence the pathways regulating the balance between protein synthesis and degradation within muscle fibres. 5 During postnatal development, this equilibrium, which determines cell Abbreviations used: ADC, apparent diffusion coefficient; ANOVA, analysis of variance; DTI, diffusion tensor imaging; DWI, diffusion-weighted imaging; EBD, Evans blue dye; FFPE, formalin-fixed, paraffin-embedded; FOV, field of view; FSE, fast spin echo; GA, gastrocnemius; G diff , diffusion gradient direction; H&E, haematoxylin and eosin; i.d., inner diameter; MRI, magnetic resonance imaging; NBF, neutral buffered formalin;...

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“…Nowadays, the development of biomultiomics is very rapid, which provides us with advantageous methods and technical means for further exploring the mechanism of DNA methylation, histone modification (Bodega et al, 2017;Creyghton et al, 2010;Yan et al, 2016), lncRNAs (Butchart et al, 2016;Guo et al, 2017;Jin et al, 2017), and circRNAs expression in the regulation of muscle formation of bovine skeletal muscle MSCs by SQLE (Cherubini et al, 2019;Legnini et al, 2017). Then, we will use our previous research results, combining with CRISPR/Cas9 technology to produce SQLE gene-edited animals (Hryhorowicz et al, 2017;J and Das, 2019), and verify the regulatory role of SQLE on animal production performance.…”

Section: The Role Of Sqle Gene On Differentiation Of Mscsmentioning

confidence: 99%

SQLEPromotes Differentiation and Apoptosis of Bovine Skeletal Muscle-Derived Mesenchymal Stem Cells

Zhang¹,

Deng²,

Lv³

et al. 2020

Cellular Reprogramming

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In this study, Squalene epoxidase (SQLE) overexpression vector was transfected into bovine skeletal musclederived mesenchymal stem/stromal cells (MSCs) to study the molecular mechanism of SQLE regulating meat quality through myogenesis. We initially profiled the expression of SQLE in cattle embryos and adults, in the muscle tissue of four different cattle varieties, and in 11 different tissues/organs of Guangxi cattle variety. Subsequently, we isolated and cultured bovine skeletal muscle-derived MSCs and detected the expression of SQLE during cell proliferation and differentiation. Then, we constructed a bovine SQLE overexpression vector and transfected it into bovine skeletal muscle-derived MSCs by liposome transfection. Cell Counting Kit-8 (CCK-8), 5-ethynyl-20-deoxyuridine (EdU), flow cytometry, immunofluorescence, and quantitative polymerase chain reaction assays were used to characterize cell proliferation and differentiation in detail. The results showed that the relative expression level of bovine SQLE gene in brain tissue was the highest, and in adult muscle tissue was significantly higher than that in embryonic stage. Especially, the expression of SQLE was significantly upregulated in cell differentiation stage. Furthermore, the proliferation, cell cycle, apoptosis, and myoblast differentiation assays indicated that SQLE significantly promoted the differentiation and apoptosis of bovine skeletal muscle-derived MSCs, but inhibited their proliferation. In conclusion, our study reveals the role of SQLE in myoblast differentiation. These results will provide new clues for the regulation network of bovine muscle development.

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The long and short of non-coding RNAs during post-natal growth and differentiation of skeletal muscles: Focus on lncRNA and miRNAs

Cited by 36 publications

References 77 publications

IncRNA AK017368 promotes proliferation and suppresses differentiation of myoblasts in skeletal muscle development by attenuating the function of miR‐30c

IncRNA AK017368 promotes proliferation and suppresses differentiation of myoblasts in skeletal muscle development by attenuating the function of miR‐30c

The effects of ageing on mouse muscle microstructure: a comparative study of time‐dependent diffusion MRI and histological assessment

SQLEPromotes Differentiation and Apoptosis of Bovine Skeletal Muscle-Derived Mesenchymal Stem Cells

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