SQLEPromotes Differentiation and Apoptosis of Bovine Skeletal Muscle-Derived Mesenchymal Stem Cells

Abstract

In this study, Squalene epoxidase (SQLE) overexpression vector was transfected into bovine skeletal musclederived mesenchymal stem/stromal cells (MSCs) to study the molecular mechanism of SQLE regulating meat quality through myogenesis. We initially profiled the expression of SQLE in cattle embryos and adults, in the muscle tissue of four different cattle varieties, and in 11 different tissues/organs of Guangxi cattle variety. Subsequently, we isolated and cultured bovine skeletal muscle-derived MSCs and detected the expression of SQLE during cell proliferation and differentiation. Then, we constructed a bovine SQLE overexpression vector and transfected it into bovine skeletal muscle-derived MSCs by liposome transfection. Cell Counting Kit-8 (CCK-8), 5-ethynyl-20-deoxyuridine (EdU), flow cytometry, immunofluorescence, and quantitative polymerase chain reaction assays were used to characterize cell proliferation and differentiation in detail. The results showed that the relative expression level of bovine SQLE gene in brain tissue was the highest, and in adult muscle tissue was significantly higher than that in embryonic stage. Especially, the expression of SQLE was significantly upregulated in cell differentiation stage. Furthermore, the proliferation, cell cycle, apoptosis, and myoblast differentiation assays indicated that SQLE significantly promoted the differentiation and apoptosis of bovine skeletal muscle-derived MSCs, but inhibited their proliferation. In conclusion, our study reveals the role of SQLE in myoblast differentiation. These results will provide new clues for the regulation network of bovine muscle development.

Understanding Myoblast Differentiation Pathways When Cultured on Electroactive Scaffolds through Proteomic Analysis

“…The Gsta2 gene encodes a glutathione S-tranferase, an enzyme which protects cells against reactive oxygen species and the products of peroxidation. Relative to the positively charge PVDF sample (Figure b), the results show that the 5 most upregulated proteins comparatively to the control were Ptgs2 , which can be associated with cell adhesion, phenotypic changes, and resistance to apoptosis (291%); Fbln2 , related to calcium ion binding (259%), which is related to muscle function; Srpr (Signal recognition particle receptor subunit alpha) (258%), which is involved in the targeting and translocation of signal sequence tagged secretory and membrane proteins across the endoplasmic reticulum; and Sqle , which has demonstrated its expression on muscle cell proliferation and differentiation being significantly down- and upregulated, respectively . So, in this study, the “poled +” condition leads to increased expression of this protein (227%), potentially boosting the cell differentiation stage in a meaningful way.…”

Fundamental Boolean network modelling for childhood acute lymphoblastic leukaemia pathways

“…When BMF is inhibited, it will activate the key gene CDC45 and then kick off the intrinsic apoptotic cascade by activating DTL, KNL1, SQLE and BRIP1, to mediate cell apoptosis. Notably, the three genes KNL1 [82], SQLE [83] and BRIP1 [84] are associated with the apoptosis of the cancer cell. However, the activation of DTL could be a serious side effect of GC related treatment because the overexpression of DTL is significantly up-regulated in cancer tissues than in normal tissues [85].…”