IncRNA AK017368 promotes proliferation and suppresses differentiation of myoblasts in skeletal muscle development by attenuating the function of miR‐30c

Abstract

Long noncoding RNAs (lncRNAs) have been reported to play diverse roles in biologic and pathologic processes, including myogenesis. We found that lncRNA is highly expressed in skeletal muscle cells. Functional analyses showed that overexpression of promoted proliferation and restrained differentiation of myoblasts; whereas inhibition of had completely opposite effects In mice, knockdown of promoted muscle hypertrophy RNA molecules of acted mechanistically as competing endogenous RNAs to target micro-RNA (miR)-30c, which was supported by the results of bioinformatics analyses and dual-luciferase reporter assays. It has been shown that lncRNA competes with trinucleotide repeat containing-6A () for miR-30c. was previously reported to promote proliferation and inhibit differentiation of myoblast cells, whereas miR-30c targets the 3'-UTR of mRNA to weaken its function. Taken together, lncRNA promotes proliferation and inhibits differentiation of myoblast cells by attenuating function of miR-30c.-Liang, T., Zhou, B., Shi, L., Wang, H., Chu, Q., Xu, F., Li, Y., Chen, R., Shen, C., Schinckel, A. P. lncRNA promotes proliferation and suppresses differentiation of myoblasts in skeletal muscle development by attenuating the function of miR-30c.

Long Non-Coding RNA RP11-789C1.1 Suppresses Epithelial to Mesenchymal Transition in Gastric Cancer Through the RP11-789C1.1/MiR-5003/E-Cadherin Axis

“…Being important members of the non-coding RNA family, lncRNAs have been found to be associated with many physiological and pathological processes, such as cell growth, differentiation, development, and tumorigenesis [23][24][25]. For their role in tumorigenesis, the aberrant expression of lncRNAs has an impact not only on cancer cell proliferation and metastasis and as prognostic factors [26] but also on EMT, tumor drug resistance, and cancer stem cells [27,28].…”

Non-coding RNAs Shaping Muscle

“…Since the discovery of linc-MD1, which was one of the first miRNA sponges to be identified, additional muscle relevant ceRNAs have been described. Recent examples include lnc-mg (Zhu et al, 2017), AK017386 (Liang et al, 2018), LncMUMA (Zhang et al, 2018a), and MAR1 (Zhang et al, 2018b) lncRNAs. Lnc-mg is a 5 ′ -capped and polyadenylated ncRNA whose ablation (i) in vitro, reduces the capacity of murine satellite cells (MuSCs) to differentiate and (ii) in vivo, results in muscular atrophy associated to reduced muscle endurance.…”

Roles of lncRNAs and circRNAs in regulating skeletal muscle development

“…33 linc-MD1 is reported to sponge miR-133 and miR-135 to regulate the expression of MAML1 and MEF2C in its activation of muscle-specific gene expression. The lncRNA AK017368 promotes proliferation and suppresses differentiation of myoblasts in skeletal muscle development by attenuating the function of miR-30c, and lncRNA AK017368 competes with a trinucleotide repeat containing-6A for miR-30c.…”

“…The lncRNA AK017368 promotes proliferation and suppresses differentiation of myoblasts in skeletal muscle development by attenuating the function of miR-30c, and lncRNA AK017368 competes with a trinucleotide repeat containing-6A for miR-30c. 33 linc-MD1 is reported to sponge miR-133 and miR-135 to regulate the expression of MAML1 and MEF2C in its activation of muscle-specific gene expression. 34,35 Furthermore, GosB, targeted by miR-133a targeting, is sponged by muscle differentiation-associated lncRNA (MDNCR) which promotes cell differentiation in primary myoblasts from cattle.…”