The lncRNA H19/miR-675 axis regulates myocardial ischemic and reperfusion injury by targeting PPARα

Luo, Hong; Wang, Jing; Liu, Donghai; Zang, Suhua; Ma, Ning; Zhao, Longshuan; Zhang, Liang; Zhang, Xin; Qiao, Chenhui

doi:10.1016/j.molimm.2018.11.011

Cited by 71 publications

(61 citation statements)

References 36 publications

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“…This model has been proved to be important in DCM. For example, lncRNA-H19 is involved in myocardial inflammation via regulation of microRNA-675 (Luo et al, 2019); Lnc-kcnq1ot1 could act as a ceRNA for miR-214-3p to regulate the expression of caspase-1 ; And lncRNA-MIAT, serving as a competing endogenous RNA by sponging miR-22-3p regulates the gene expression of death-associated protein kinase-2, which consequently leads to cardiomyocyte apoptosis (Xiang et al, 2017). In our study, we first reported that lncRNA-MIAT, acting as microRNA sponges, attenuates inhibitory effects of miR-214-3p on IL-17 production, leading to cardiac fibrosis in the heart.…”

Section: Discussionmentioning

confidence: 99%

LncRNA-MIAT-Mediated miR-214-3p Silencing Is Responsible for IL-17 Production and Cardiac Fibrosis in Diabetic Cardiomyopathy

Mai

et al. 2020

Front. Cell Dev. Biol.

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As an important complication of diabetes mellitus, diabetic cardiomyopathy (DCM) is characterized by a silent development in its earlier stage and a deficient cardiomyocyte contractility in its late stage. So far, little advance has been achieved to reverse this pathological change. LncRNAs are defined as a large cluster of RNAs without the function of encoding proteins, but have the capacity in controlling gene expression. Interleukin-17 (IL-17), a proinflammatory cytokine, is a key regulator of host inflammation. Clinically, it plays a crucial role in the pathogenesis of cardiac interstitial fibrosis. In this study, we reported that high glucose-induced lncRNA-MIAT upregulation is responsible for proinflammatory IL-17 production in cardiomyocytes. The underlying mechanism is likely due to that lncRNA-MIAT specific attenuates miR-214-3p-mediated inhibitory effect on IL-17 expression. As a result, attenuated IL-17 expression significantly ameliorate cardiac fibrosis, followed by improvement of cardiac contractility. Taken together, our study first suggests that lncRNA-MIAT plays a key role in DCM and targeting lncRNA-MIAT may become a potential strategy to treat DCM.

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Section: Discussionmentioning

confidence: 99%

LncRNA-MIAT-Mediated miR-214-3p Silencing Is Responsible for IL-17 Production and Cardiac Fibrosis in Diabetic Cardiomyopathy

Mai

et al. 2020

Front. Cell Dev. Biol.

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“…Recent findings have also reported the effects of H19 in pathological cardiac hypertrophy (L. Liu et al, 2016), DCM (X. Li et al, 2016), and dilated cardiomyopathy (Zhang, Zhang, Xu, Chen, & Zhou, 2017). H. Luo et al (2019) confirmed the upregulation of H19 and the protective role of H19 in a mouse model of myocardial I/R injury, suggesting that H19 may be a new therapeutic target for treating myocardial I/R injury. In this study, we established a mouse model of myocardial IP and a cellular model of H 2 O 2 ‐PC to investigate the potential role of H19 during myocardial IP.…”

Section: Discussionmentioning

confidence: 91%

“…Early studies have suggested that H19 may posttranscriptionally regulate specific proteins (He et al, 2019). Meanwhile, H19 has been proven to be the primary transcript for microRNA‐675 (Dykes & Emanueli, 2017; Luo et al, 2019; Park, Mitra, Rahat, Kim, & Pfeifer, 2017). H19/miR‐675 were found to be associated with different types of diseases (Fan, Peng, Liang, Yang, & Zhang, 2019; Luo et al, 2019; Muller et al, 2019; Wang et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…H19 has multiple functions, and some recent studies have reported the regulatory roles of H19 in cellular biological processes (Cai et al, 2016). Notably, in addition to critically regulating tumor development and tumor metastasis, H19 was found to be involved in a variety of cardiac‐related diseases, such as diabetic cardiomyopathy (DCM; X. Li et al, 2016), acute myocardial infarction (Gong et al, 2017; Zhou et al, 2018), cardiac fibrosis (Huang, Tian, Yang, & Guo, 2017), and myocardial I/R injury (H. Luo et al, 2019). X. Zhang et al (2018) have indicated that H19 acts as a mediator functioning in hypoxic postconditioning (H/Post), protecting senescent cardiomyocytes against hypoxia/reoxygenation (H/R) injury.…”

Section: Introductionmentioning

confidence: 99%

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LncRNA H19 is involved in myocardial ischemic preconditioning via increasing the stability of nucleolin protein

Chen

Liu

Tang

et al. 2020

Journal Cellular Physiology

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Myocardial ischemic preconditioning (IP) is defined as a brief period of myocardial ischemia/reperfusion (I/R) that significantly reduces injury during the subsequent exposure to long-term I/R. However, the underlying mechanisms of myocardial IP are yet to be elucidated. This study investigated the expression and roles of long noncoding RNA (lncRNA) H19 in myocardial IP in vitro and in vivo. LncRNA H19 expression levels were analyzed by quantitative reverse-transcription polymerase chain reaction, cell viability was determined by the Cell Counting Kit-8 assay, apoptosis was evaluated based on the caspase 3 activity, and RNA immunoprecipitation was performed to examine the interaction between lncRNA H19 and nucleolin. The results of this study showed that lncRNA H19 expression was significantly upregulated in mouse hearts subjected to myocardial IP, in rat H9C2 cells exposed to H 2 O 2 preconditioning (H 2 O 2 -PC), and in neonatal rat cardiomyocytes subjected to hypoxia preconditioning. H19 knockdown abrogated the H 2 O 2 -PC-mediated protection in cardiomyocytes evidenced by the decreased cell viability and increased caspase-3 activity. Conversely, H19 overexpression enhanced the protective role of H 2 O 2 -PC in cardiomyocytes. In addition, H19 overexpression increased the expression of nucleolin, whereas H19 ablation abrogated H 2 O 2 -PC-induced upregulation of nucleolin in cardiomyocytes. Furthermore, H19 overexpression increased the stabilization of nucleolin; an interaction between H19 and nucleolin was identified using the RNA-protein interaction studies. Furthermore, nucleolin small interfering RNA relieved the protective role of lncRNA H19. These findings demonstrated that the lncRNA H19 is involved in myocardial IP via increasing the stability of nucleolin protein and lncRNA H19 may represent a potential therapeutic target for the treatment of the myocardial injury.

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“…37 The detrimental effects of H19 in myocytes involve production of miR-675 (the mature gene product of H19), and downregulation of the expression of peroxisome proliferator-activated receptor-α. 37…”

Section: Lncrnas With Pro-apoptotic Effectsmentioning

confidence: 99%

Long noncoding RNAs: Important participants and potential therapeutic targets for myocardial ischaemia reperfusion injury

Ding

Chan

Liu

et al. 2020

Clin Exp Pharma Physio

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Myocardial ischaemia reperfusion (I/R) injury is one of the leading causes of coronary artery disease-associated morbidity and mortality. While different strategies have been used to limit I/R injuries, cardiac functions often do not recover to the normal level as anticipated. Recent studies have pointed to important roles of long noncoding RNAs (lncRNAs) in the development of myocardial I/R injury. LncRNA is a class of RNA molecules of more than 200 nucleotides in length which are not translated into proteins. I/R causes dysregulation of lncRNA expression in cardiomyocytes, thereby How to cite this article: Ding Y-M, Chan EC, Liu L-C, et al. Long noncoding RNAs: Important participants and potential therapeutic targets for myocardial ischaemia reperfusion injury.

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The lncRNA H19/miR-675 axis regulates myocardial ischemic and reperfusion injury by targeting PPARα

Cited by 71 publications

References 36 publications

LncRNA-MIAT-Mediated miR-214-3p Silencing Is Responsible for IL-17 Production and Cardiac Fibrosis in Diabetic Cardiomyopathy

LncRNA-MIAT-Mediated miR-214-3p Silencing Is Responsible for IL-17 Production and Cardiac Fibrosis in Diabetic Cardiomyopathy

LncRNA H19 is involved in myocardial ischemic preconditioning via increasing the stability of nucleolin protein

Long noncoding RNAs: Important participants and potential therapeutic targets for myocardial ischaemia reperfusion injury

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