The lectin ArtinM activates RBL-2H3 mast cells without inducing degranulation

Abstract

Mast cells are connective tissue resident cells with morphological and functional characteristics that contribute to their role in allergic and inflammatory processes, host defense and maintenance of tissue homeostasis. Mast cell activation results in the release of pro-inflammatory mediators which are largely responsible for the physiological functions of mast cells. The lectin ArtinM, extracted from Artocarpus heterophyllus (jackfruit), binds to D-manose, thus inducing degranulation of mast cells. ArtinM has several immunomodulatory properties including acceleration of wound healing, and induction of cytokine release. The aim of the present study was to investigate the role of ArtinM in the activation and proliferation of mast cells. The rat mast cell line RBL-2H3 was used throughout this study. At a low concentration (0.25μg/mL), ArtinM induced mast cell activation and the release of IL-6 without stimulating the release of pre-formed or newly formed mediators. Additionally, when the cells were activated by ArtinM protein tyrosine phosphorylation was stimulated. The low concentration of ArtinM also activated the transcription factor NFkB, but not NFAT. ArtinM also affected the cell cycle and stimulated cell proliferation. Therefore, ArtinM may have therapeutic applications by modulating immune responses due to its ability to activate mast cells and promote the release of newly synthesized mediators. Additionally, ArtinM could have beneficial effects at low concentrations without degranulating mast cells and inducing allergic reactions.

Sodium Sulfite-Induced Mast Cell Pyroptosis and Degranulation

“…12 RBL-2H3 cells are mucosal mast cells that have been widely used for studies of degranulation. 41,42 Presently, we found that sodium sulfite dose-dependent induced histamine and β-Hexosaminidase release, which could be inhibited by NAC and MCC950. Another research has shown that sodium sulfite induced degranulation of RBL- 2H3 cells and oxidant stress, which is consistent with our results.…”

Biological study of skin wound treated with Alginate/Carboxymethyl cellulose/chorion membrane, diopside nanoparticles, and Botox A

“…Thus, a proper wound dressing should possess a highly porous structure, desired degradation rate, and minimal cytotoxicity effect besides high biocompatibility to reduce immunogenicity 6,7 . Meanwhile, there are various marketed wound care products to use in clinical application, effective wound treatment to recover skin function following chronic and severe skin damage still needs serious medical interventions, thus, a proper wound dressing is highly important to design to induce neovascular genesis and neocollagenesis besides restoration of skin integrity and function 8,9 .…”