Biological study of skin wound treated with Alginate/Carboxymethyl cellulose/chorion membrane, diopside nanoparticles, and Botox A

Abstract

A hydrogel-based wound dressing with desirable properties is necessary for achieving functional skin integrity post-injury. This study focuses on preparing a hydrogel using Alginate/Carboxymethyl cellulose (Alg/CMC) as a base material. To evaluate its regenerative effects on full-thickness wounds, diopside nanoparticles and Botulinum toxin A (BTX-A) were incorporated into the hydrogel along with chorion membrane. The diopside nanoparticles (DNPs) act as a proangiogenic factor, promoting proliferation and regulating inflammation, while the chorion membrane facilitates these processes. Additionally, BTX-A prevents scar formation and aids in wound closure. The nanoparticles and hydrogel were characterized using various techniques, and their cytocompatibility was assessed. In vivo studies and quantitative polymerase chain reaction analysis showed that wound area reduction was significant after two weeks of treatment with the Alg/CMC/ChNPs/DNPs/BTX-A hydrogel. Overall, this scaffold demonstrated potential for promoting tissue regeneration and new epithelization formation, making it a promising candidate for enhancing skin restoration in wound treatments.

Intradermal Injection of Hybrid Complexes of High- and Low-Molecular-Weight Hyaluronan: Where Do We Stand and Where Are We Headed in Regenerative Medicine?

“…Regenerative medicine is defined as the area of medicine that aims to restore organs or tissues that are impaired by pathological conditions or by trauma [ 51 , 52 , 53 ]. In this regard, potential therapeutic strategies to counteract the action of several inflammatory conditions and diseases are represented by novel procedures based on cellular therapies, principally involving the use of stem cells [ 54 , 55 ] or by several biomaterial formulations [ 56 , 57 ]. In particular, skin defects induced by trauma, burns, and unhealing chronic wound are a potential target to be treated thanks to the use of both cell-based and cell-free therapies [ 58 , 59 ].…”