As one of the simplest molecules containing a peptide bond, N-methyl formamide (HCONHCH 3) represents a potential key molecule involved in the peptide bond polymerization in extraterrestrial ices. Detected tentatively toward the star-forming region Sgr B2(N2), the synthetic pathways have previously been elusive. By exploiting isomer-selective detection of the reaction products via photoionization, coupled with reflectron time-of-flight mass spectrometry (PI-ReTOF-MS), we present compelling evidence for the formation of N-methyl formamide (HCONHCH 3) in astrochemically relevant ice mixtures of methylamine (CH 3 NH 2) and carbon monoxide (CO), upon irradiation with energetic electrons as generated in the track of galactic cosmic ray particles (GCRs) penetrating interstellar ices. As one of the simplest molecules containing a peptide bond (-CO-NH-), N-methyl formamide could represent a benchmark involved in radiation-induced peptide bond polymerization in extraterrestrial ices, and thus bring us closer to revealing where in the Universe the molecular precursors linked to the origins of life might have been synthesized.
Bacterial extracellular proteases from six strains of marine bacteria and seven strains of terrestrial bacteria were prepared through fermentation. Proteases were analyzed through substrate immersing zymography and used to hydrolyze the collagen and muscle proteins from a salmon skin byproduct, respectively. Collagen could be degraded much more easily than muscle protein, but it commonly showed weaker antioxidant capability. The hydrolysate of muscle proteins was prepared with crude enzymes from Pseudoalteromonas sp. SQN1 displayed the strongest activity of antioxidant in DPPH and hydroxyl radical scavenging assays (74.06% ± 1.14% and 69.71% ± 1.97%), but did not perform well in Fe2+ chelating assay. The antioxidant fractions were purified through ultrafiltration, cation exchange chromatography, and size exclusion chromatography gradually, and the final purified fraction U2-S2-I displayed strong activity of antioxidant in DPPH, hydroxyl radical scavenging assays (IC50 = 0.263 ± 0.018 mg/mL and 0.512 ± 0.055 mg/mL), and oxygen radical absorption capability assay (1.960 ± 0.381 mmol·TE/g). The final purified fraction U2-S2-I possessed the capability to protect plasmid DNA against the damage of hydroxyl radical and its effect was similar to that of the original hydrolysis product. It indicated that U2-S2-I might be the major active fraction of the hydrolysate. This study proved that bacterial extracellular proteases could be utilized in hydrolysis of a salmon byproduct. Compared with collagen, muscle proteins was an ideal material used as an enzymatic substrate to prepare antioxidant peptides.
This study developed novel oral delivery systems for the encapsulation, protection, and controlled release of hydrophobic and hydrophilic bioactive compounds based on l-arginine- or l-lysine-functionalized chitosan–casein nanoparticles.
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