The Lanata trichome mutation increases stomatal conductance and reduces leaf temperature in tomato

Gasparini, Karla; Silva, Mateus F. da; Costa, Lucas Cavalcante da; Martins, Samuel C. V.; Ribeiro, Dimas M.; Peres, Lázaro Eustáquio Pereira; Zsögön, Agustín

doi:10.1016/j.jplph.2021.153413

Cited by 10 publications

(4 citation statements)

References 61 publications

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“…Strikingly, the population growth of A. lycopersici in the absence of predators was even higher on wild type than on hairless plants, reinforcing the notion that direct (trichome‐based) defences are no universal panacea against all herbivores. Albeit speculatively, abiotic factors at the leaf surface, such as temperature or reflected radiation, are associated with the absence/presence of trichomes (Gasparini et al, 2021 ; Karabourniotis et al, 2020 ), which may result in an unfavourable microenvironment for this extremely small mite species on hairless plants. In addition to the tomato russet mite, other specialist herbivores of sticky plants have also shown decreased densities on genotypes that lack resistant glandular trichomes (Krimmel & Pearse, 2014 ; van Dam & Hare, 1998 ).…”

Section: Discussionmentioning

confidence: 99%

Enhanced top‐down control of herbivore population growth on plants with impaired defences

et al. 2022

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1. Herbivore densities can be regulated by bottom-up and top-down forces such as plant defences and natural enemies, respectively. These forces can interact with each other to increase plant protection against herbivores; however, how much complementarity exists between bottom-up and top-down forces still remains to be fully elucidated. Particularly, because plant defences can hinder natural enemies, how these interactions affect herbivore performance and dynamics remains elusive.2. To address this topic, we performed laboratory and greenhouse bioassays with herbivorous mite pests and predatory mites on mutant tomato plants that lack defensive hairs on stems and leaves. Particularly, we investigated the behaviour and population dynamics of different phytophagous mite species in the absence and presence of predatory mites.3. We show that predatory mites do not only perform better on tomatoes lacking defensive hairs but also that they can suppress herbivore densities better and faster on these hairless plants. Hence, top-down control of herbivores by natural enemies more than compensated the reduced bottom-up herbivore control by plant defences. 4. Our results lead to the counter-intuitive insight that removing, instead of introducing, plant defence traits can result in superior protection against important pests through biological control.

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Section: Discussionmentioning

confidence: 99%

Enhanced top‐down control of herbivore population growth on plants with impaired defences

et al. 2022

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“…The opposite responses of tomato and Arabidopsis to increased temperature intrigued us. While there are numerous reports of different plants exhibiting stomatal opening and closing responses to changing temperature (34)(35)(36), stomatal production or pattern responses are less clear. The Arabidopsis accession used in Lau (2018) was Col-0, a plant from a cool moist climate.…”

Section: Slspch Cis-regulatory Mutants Display Altered Responsiveness...mentioning

confidence: 99%

Targeting editing of tomatoSPEECHLESScis-regulatory regions generates plants with altered stomatal density in response to changing climate conditions

Nir,

Budrys,

Smoot

et al. 2023

Preprint

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Flexible developmental programs enable plants to customize their organ size and cellular composition. In leaves of eudicots, the stomatal lineage produces two essential cell types, stomata and pavement cells, but the total numbers and ratio of these cell types can vary. Central to this flexibility is the stomatal lineage initiating transcription factor, SPEECHLESS (SPCH). Here we show, by multiplex CRISPR/Cas9 editing ofSlSPCH cis-regulatory sequences in tomato, that we can identify variants with altered stomatal development responses to light and temperature cues. Analysis of tomato leaf development across different conditions, aided by newly-created tools for live-cell imaging and translational reporters of SlSPCH and its paralogues SlMUTE and SlFAMA, revealed the series of cellular events that lead to the environmental change-driven responses in leaf form. Plants bearing the novel SlSPCH variants generated in this study are powerful resources for fundamental and applied studies of tomato resilience in response to climate change.Significance statementPlants can change their shape, size and cellular composition in response to environmental cues. Here, by precise gene editing of a core stomatal development regulator gene in tomato, we generate new alleles with enhanced or dampened responses to light and temperature cues. Combined with live imaging of development, we show the genetic and cellular pathways that contribute to customization of the leaf epidermis, and how this could lead to better climate-adapted varieties.

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“…The EB equation is based on physical processes that affect T l , including changes in transpiration. By estimating g s from the EB equation, it is possible to detect stomatal closures resulting from various biotic and abiotic stresses (Gasparini et al, 2021; Merlot et al, 2002; Takahashi et al, 2015), whereas many empirical models of g s require additional measurements and modelling to represent them accurately. Thus, EB analysis can potentially reduce the uncertainties of the MLR model and enhance its applicability.…”

Section: Introductionmentioning

confidence: 99%

Alternative method for determining leaf CO₂ assimilation without gas exchange measurements: Performance, comparison and sensitivity analysis

Kimura,

Kumagai,

Fushimi

et al. 2023

Plant Cell & Environment

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We present an alternative method to determine leaf CO2 assimilation rate (An), eliminating the need for gas exchange measurements in proximal and remote sensing. This method combines the Farquhar–von Caemmerer–Berry photosynthesis model with mechanistic light reaction (MLR) theory and leaf energy balance (EB) analysis. The MLR theory estimates the actual electron transport rate (J) by leveraging chlorophyll fluorescence via pulse amplitude‐modulated fluorometry for proximal sensing or sun‐induced chlorophyll fluorescence measurements for remote sensing, along with spectral reflectance. The EB equation is used to directly estimate stomatal conductance from leaf temperature. In wheat and soybean, the MLR‐EB model successfully estimated An variations, including midday depression, under various environmental and phenological conditions. Sensitivity analysis revealed that the leaf boundary layer conductance (gb) played an equal, if not more, crucial role compared to the variables for J. This was primarily caused by the indirect influence of gb through the EB equation rather than its direct impact on convective CO2 exchange on the leaf. Although the MLR‐EB model requires an accurate estimation of gb, it can potentially reduce uncertainties and enhance applicability in photosynthesis assessment when gas exchange measurements are unavailable.

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The Lanata trichome mutation increases stomatal conductance and reduces leaf temperature in tomato

Abstract: A monogenic mutation in tomato increases trichome density and optimizes gas exchange and leaf temperature

Cited by 10 publications

References 61 publications

Enhanced top‐down control of herbivore population growth on plants with impaired defences

Enhanced top‐down control of herbivore population growth on plants with impaired defences

Targeting editing of tomatoSPEECHLESScis-regulatory regions generates plants with altered stomatal density in response to changing climate conditions

Alternative method for determining leaf CO₂ assimilation without gas exchange measurements: Performance, comparison and sensitivity analysis

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The Lanata trichome mutation increases stomatal conductance and reduces leaf temperature in tomato

Abstract: A monogenic mutation in tomato increases trichome density and optimizes gas exchange and leaf temperature

Cited by 10 publications

References 61 publications

Enhanced top‐down control of herbivore population growth on plants with impaired defences

Enhanced top‐down control of herbivore population growth on plants with impaired defences

Targeting editing of tomatoSPEECHLESScis-regulatory regions generates plants with altered stomatal density in response to changing climate conditions

Alternative method for determining leaf CO2 assimilation without gas exchange measurements: Performance, comparison and sensitivity analysis

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Product

Resources

About

Alternative method for determining leaf CO₂ assimilation without gas exchange measurements: Performance, comparison and sensitivity analysis