Targeting editing of tomatoSPEECHLESScis-regulatory regions generates plants with altered stomatal density in response to changing climate conditions
Ido Nir,
Alanta Budrys,
N. Katherine Smoot
et al.
Abstract:Flexible developmental programs enable plants to customize their organ size and cellular composition. In leaves of eudicots, the stomatal lineage produces two essential cell types, stomata and pavement cells, but the total numbers and ratio of these cell types can vary. Central to this flexibility is the stomatal lineage initiating transcription factor, SPEECHLESS (SPCH). Here we show, by multiplex CRISPR/Cas9 editing ofSlSPCH cis-regulatory sequences in tomato, that we can identify variants with altered stoma… Show more
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