The Isolation of Stem Cells from Human Deciduous Teeth Pulp Is Related to the Physiological Process of Resorption

Bernardi, Lisiane; Luisi, Simone Bonato; Fernandes, Raquel Arrieche; Dalberto, Tiago Pires; Valentim, Lauren Martins; Chies, José Artur Bogo; Fossati, Anna Christina Medeiros; Pranke, Patrícia

doi:10.1016/j.joen.2011.04.010

Cited by 65 publications

(51 citation statements)

References 36 publications

(63 reference statements)

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“…Thus, different cell markers have been described and tested in order to define this population of cells. In our study, the isolated pulp cells showed positive expression for the mesenchymal stem cell markers CD44, CD73, CD90, and STRO-1, and showed no expression for the hematopoietic marker CD45, agreeing with previous reports 6,11,12,17,19 . STRO-1 shows a variable expression level by flow cytometry in human pulp cells (1.2% to 9.56%), despite being considered a genuine marker of mesenchymal stem cells 9,18,20 .…”

Section: Discussionsupporting

confidence: 93%

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Isolation and culture of stem cells derived from dental pulp of permanent teeth: a successful method

Machado

Carvalho

Campos

et al. 2015

Rev. da Fac. de Odontologia, UPF

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Section: Discussionsupporting

confidence: 93%

“…A 90% confluence of DPSCs is usually reached after 10 to 14 days of culture, with the explant method 12,17 . Our results showed similar 90% cell confluence after two weeks of tissue culture, demonstrating the effectiveness of the isolation method employed.…”

Section: Discussionmentioning

confidence: 99%

Isolation and culture of stem cells derived from dental pulp of permanent teeth: a successful method

Machado

Carvalho

Campos

et al. 2015

Rev. da Fac. de Odontologia, UPF

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“…MSCs were isolated from human deciduous teeth pulp (n=5) and characterized, as described by Bernardi et al 15 after approval by the Ethics Committee of the Federal University of Rio Grande do Sul.…”

Section: Materials and Methods Culture Of Mscs And Preparation Of Celmentioning

confidence: 99%

Association of electrospinning with electrospraying: a strategy to produce 3D scaffolds with incorporated stem cells for use in tissue engineering

Braghirolli¹,

Zamboni²,

Acasigua³

et al. 2015

IJN

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In tissue engineering, a uniform cell occupation of scaffolds is crucial to ensure the success of tissue regeneration. However, this point remains an unsolved problem in 3D scaffolds. In this study, a direct method to integrate cells into fiber scaffolds was investigated by combining the methods of electrospinning of fibers and bioelectrospraying of cells. With the associating of these methods, the cells were incorporated into the 3D scaffolds while the fibers were being produced. The scaffolds containing cells (SCCs) were produced using 20% poly(lactide- co -glycolide) solution for electrospinning and mesenchymal stem cells from deciduous teeth as a suspension for bioelectrospraying. After their production, the SCCs were cultivated for 15 days at 37°C with an atmosphere of 5% CO 2 . The 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide test demonstrated that the cells remained viable and were able to grow between the fibers. Scanning electron microscopy showed the presence of a high number of cells in the structure of the scaffolds and confocal images demonstrated that the cells were able to adapt and spread between the fibers. Histological analysis of the SCCs after 1 day of cultivation showed that the cells were uniformly distributed throughout the thickness of the scaffolds. Some physicochemical properties of the scaffolds were also investigated. SCCs exhibited good mechanical properties, compatible with their handling and further implantation. The results obtained in the present study suggest that the association of electrospinning and bioelectrospraying provides an interesting tool for forming 3D cell-integrated scaffolds, making it a viable alternative for use in tissue engineering.

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“…compared the enzyme digestion and outgrowth methods and found that cells isolated by enzyme digestion had a higher proliferation rate than those isolated by the other methods. In this study, the enzymatic digestion method used in the studies of Yu, et al [13], Bernardi, et al [14], Werle, et al [2], and their colleagues, was employed.…”

Section: Discussionmentioning

confidence: 99%

Isolation, immunophenotypic characterization and pluripotency of dental pulp stem cells

Luisi¹,

Filho²,

Pranke³

2017

Dent Oral Craniofac Res

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Objective: The aim of this study has been the isolation and characterization of stem cells from dental pulp (DPSCs) in culture. Methods:The primary DPSCs cultures were obtained from human third molars. Immediately after extraction, the teeth were placed in Dulbecco's modified Eagle medium (DMEM) culture medium supplemented with fetal bovine serum and antibiotics. In a laminar flow, the pulp was removed from the tooth, incubated with collagenase for 2 hours and placed on a culture plate. Phenotypic characterization and cell pluripotency was performed in the fifth passage (P5). To evaluate the expression of surface markers, the cells were incubated with antibodies against CD14, CD29, CD34, CD44, CD45, CD73, CD90, CD105 and HLA-DR antigens. For induction of cell differentiation in vitro, 10 4 cells/cm 2 were plated in 12-well plates and cultivated in appropriate media for osteogenic, adipogenic and condrogencic differentiation after reaching at least 70% confluence. Results:The cells were positive above 95% for characteristic markers of the mesenchymal stem cells CD29, CD44, CD73 and CD90. In contrast, there was a low percentage of positivity (up to 1.1%) for the characteristic markers of hematopoietic cells such as CD14, CD34, CD45, CD184 and HLA-DR. Adipogenic differentiation was visualized by staining lipid vacuoles with Oil Red. Bone differentiation was visualized by staining calcium deposits with Alizarin Red. No chondrogenic differentiation was observed. Conclusions:The isolated cells were adherent to plastic, positive for the characteristic markers of mesenchymal stem cells and, therefore, an alternative source for tissue engineering studies.

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The Isolation of Stem Cells from Human Deciduous Teeth Pulp Is Related to the Physiological Process of Resorption

Cited by 65 publications

References 36 publications

Isolation and culture of stem cells derived from dental pulp of permanent teeth: a successful method

Isolation and culture of stem cells derived from dental pulp of permanent teeth: a successful method

Association of electrospinning with electrospraying: a strategy to produce 3D scaffolds with incorporated stem cells for use in tissue engineering

Isolation, immunophenotypic characterization and pluripotency of dental pulp stem cells

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