Isolation and culture of stem cells derived from dental pulp of permanent teeth: a successful method

Machado, Cíntia de Vasconcellos; Carvalho, Carolina Montagn; Campos, Taís M.; Passos, Sara; Telles, Paloma Dias da Silva

doi:10.5335/rfo.v20i2.4655

Cited by 1 publication

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References 23 publications

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“…Vasconcellos Machado C et al conducted a similar study in 2015, focusing on the first generation of DPSCs obtained through explant technology ( de Vasconcellos Machado et al, 2015 ). Their findings align with our observations, confirming the favorable characteristics of stem cells obtained through the explant method.…”

Section: Discussionmentioning

confidence: 99%

How to make full use of dental pulp stem cells: an optimized cell culture method based on explant technology

Wu,

Sun,

Wang

et al. 2024

Front. Bioeng. Biotechnol.

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Introduction:Dental pulp stem cells from humans possess self-renewal and versatile differentiation abilities. These cells, known as DPSC, are promising for tissue engineering due to their outstanding biological characteristics and ease of access without significant donor site trauma. Existing methods for isolating DPSC mainly include enzyme digestion and explant techniques. Compared with the enzymatic digestion technique, the outgrowth method is less prone to cell damage and loss during the operation, which is essential for DPSC with fewer tissue sources.Methods:In order to maximize the amount of stem cells harvested while reducing the cost of DPSC culture, the feasibility of the optimized explant technique was evaluated in this experiment. Cell morphology, minimum cell emergence time, the total amount of cells harvested, cell survival, and proliferative and differentiation capacity of DPSC obtained with different numbers of explant attachments (A1-A5) were evaluated.Results:There was a reduction in the survival rate of the cells in groups A2-A5, and the amount of harvested DPSC decreased in A3-A5 groups, but the DPSC harvested in groups A1-A4 had similar proliferative and differentiation abilities. However, starting from group A5, the survival rate, proliferation and differentiation ability of DPSC decreased significantly, and the adipogenic trend of the cells became more apparent, indicating that the cells had begun to enter the senescence state.Discussion:The results of our study demonstrated that the DPSC obtained by the optimized explant method up to 4 times had reliable biological properties and is available for tissue engineering.

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Section: Discussionmentioning

confidence: 99%

How to make full use of dental pulp stem cells: an optimized cell culture method based on explant technology

Wu,

Sun,

Wang

et al. 2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

Isolation and culture of stem cells derived from dental pulp of permanent teeth: a successful method

Abstract: Objective: To establish cultures of cells from the pulp of permanent teeth by the explant method assessing pa-

Cited by 1 publication

References 23 publications

How to make full use of dental pulp stem cells: an optimized cell culture method based on explant technology

How to make full use of dental pulp stem cells: an optimized cell culture method based on explant technology

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