The isolation of secondary metabolites and in vitro potent anti-cancer activity of clerodermic acid from Enicosanthum membranifolium

Efdi, Mai; Itoh, Tomohiro; Akao, Yukihiro; Nozawa, Yoshinori; Koketsu, Mamoru; Ishihara, Hideharu

doi:10.1016/j.bmc.2007.03.051

Cited by 24 publications

(16 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previously, we have reported that clerodermic acid isolated from an Indonesian plant Enicosanthum membranifolium demonstrated unique antileukemic activity. Clerodermic acid-induced growth inhibition observed in HL-60 cells was mainly due to caspase-independent apoptosis 17 . Therefore, we were interested in investigating the effects of the isolated diterpenes against HL-60 cells.…”

Section: Resultsmentioning

confidence: 95%

“…Cells undergoing apoptosis display profound structural changes, including a rapid blebbing of the plasma membrane and nuclear disintegration. In order to investigate the mechanisms of these compounds, we observed morphological changes in the cells stained with Hoechst 33342 17 . At 25 μM concentration of compounds 2 and 3, HL-60 cells showed different shapes compared with the control cells C which exhibited general pattern of cell growth and normal morphology.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Clerodane Diterpenes Isolated from Polyalthia longifolia Induce Apoptosis in Human Leukemia HL-60 Cells

et al. 2013

Self Cite

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Clerodane Diterpenes Isolated from Polyalthia longifolia Induce Apoptosis in Human Leukemia HL-60 Cells

et al. 2013

Self Cite

View full text Add to dashboard Cite

“…For the details, see our recent paper. 10) Extraction and Purification of FA from E. cupulare Air-dried bark of E. cupulare (KING) AIRY-SHAW (5 kg) was finely ground and macerated at room temperature in methanol. The mixture was subsequently filtered and concentrated in vacuo to give the methanol extract (185 g).…”

Section: Methodsmentioning

confidence: 99%

N-trans-Feruloyltyramine as a Melanin Biosynthesis Inhibitor

Efdi

Ohguchi

Akao

et al. 2007

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

In this study, we examined the effect of N-trans-feruloyltyramine (FA) on melanogenesis in mouse B16 melanoma cells. Melanogenesis was inhibited by FA in a dose-dependent manner. FA exhibited a greater potency than kojic acid as a standard inhibitor of melanogenesis. Moreover, treatment of B16 melanoma cells with FA was found to cause marked decreases in the expression levels of tyrosinase. FA-induced downregulation of tyrosinase resulted in suppression of melanin biosynthesis in murine B16 melanoma cells.

show abstract

“…The plant extracts used in the treatments play a therapeutic role in many diseases due to their metabolites. Excess of the amount of metabolite and carrying the appropriate features of these metabolites are desirable properties for plants in the treatment of diseases (Efdi et al 2007;Shen et al 2007;Russo et al 2010;Efferth et al 2011).…”

Section: Introductionmentioning

confidence: 99%

In vitroantitumor activities of the lichen compounds olivetoric, physodic and psoromic acid in rat neuron and glioblastoma cells

Emsen

Aslan

Toğar

et al. 2015

Pharmaceutical Biology

View full text Add to dashboard Cite

Context Since methods utilised in the treatment of glioblastoma multiforme (GBM) are inadequate and have too many side effects, usage of herbal products in the treatment process comes into prominence. Lichens are symbiotic organisms used for medicinal purposes for many years. There are various anticancer treatments about components of two lichen species used in the present study. Objective Antitumor potential of three lichen secondary metabolites including olivetoric acid (OLA) and physodic acid (PHA) isolated from Pseudevernia furfuracea (L.) Zopf (Parmeliaceae) and psoromic acid (PSA) isolated from Rhizoplaca melanophthalma (DC.) Leuckert (Lecanoraceae) were investigated on human U87MG-GBM cell lines and primary rat cerebral cortex (PRCC) cells for the first time. Materials and methods PRCC cells used as healthy brain cells were obtained from SpragueDawley rats. The treatments were carried out on the cells cultured for 48 h. Cytotoxic effects of different concentrations (2.5, 5, 10, 20 and 40 mg/L) of metabolites on the cells were determined via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) analyses. Total antioxidant capacity (TAC) and total oxidant status (TOS) parameters were used for assessing oxidative alterations. Oxidative DNA damage potentials of metabolites were investigated via evaluating 8-hydroxy-2 0 -deoxyguanosine (8-OH-dG) levels. Results Median inhibitory concentration (IC 50 ) values of OLA, PHA and PSA were 125.71, 698.19 and 79.40 mg/L for PRCC cells and 17.55, 410.72 and 56.22 mg/L for U87MG cells, respectively. It was revealed that cytotoxic effects of these metabolites showed positive correlation with concentration, LDH activity and oxidative DNA damage. Discussion and conclusion The present findings obtained in this study revealed that primarily OLA and then PSA had high potential for use in the treatment of GBM. ARTICLE HISTORY

show abstract

The isolation of secondary metabolites and in vitro potent anti-cancer activity of clerodermic acid from Enicosanthum membranifolium

Cited by 24 publications

References 24 publications

Clerodane Diterpenes Isolated from Polyalthia longifolia Induce Apoptosis in Human Leukemia HL-60 Cells

Clerodane Diterpenes Isolated from Polyalthia longifolia Induce Apoptosis in Human Leukemia HL-60 Cells

N-trans-Feruloyltyramine as a Melanin Biosynthesis Inhibitor

In vitroantitumor activities of the lichen compounds olivetoric, physodic and psoromic acid in rat neuron and glioblastoma cells

Contact Info

Product

Resources

About