<i>In vitro</i>antitumor activities of the lichen compounds olivetoric, physodic and psoromic acid in rat neuron and glioblastoma cells

Emsen, Bugrahan; Aslan, Ali; Toğar, Başak; Türkez, Hasan

doi:10.3109/13880209.2015.1126620

Cited by 63 publications

(35 citation statements)

References 58 publications

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“…Six newborn Sprague-Dawley rats were used to obtain PMGN cultures. This treatment process was performed according to previously study (Emsen et al 2016). The cerebral cortices were dissociated with Hank's Balanced Salt Solution (HBSS) (Sigma-Aldrich, Germany) + trypsin-ethylenediaminetetraacetic acid (EDTA) (0.25% trypsin, 0.02% EDTA; Sigma-Aldrich), treated with DNAse type 1 (Sigma-Aldrich) and centrifuged.…”

Section: Primary Mixed Glial-neuronal Cell Culturesmentioning

confidence: 99%

Inhibition of growth of U87MG human glioblastoma cells by Usnea longissima Ach.

Emsen

Özdemir

Engin

et al. 2019

An. Acad. Bras. Ciênc.

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Herbal medicines are efficient to reduce side effects in the fight against glioblastoma, which plays a critical role within brain cancer species. The recent studies designated for testing the effects of lichens that have shown numerous anticancer activities on glioblastoma so far. In the present study, different concentrations of water extract obtained from Usnea longissima Ach. were used in order to determine cytotoxic (via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase tests), antioxidant (via total antioxidant capacity test), pro-oxidant (via total oxidant status test) and genotoxic (via 8-hydroxy-2′-deoxyguanosine test) effects of them on human U87MG-glioblastoma cancer cell lines. Primary mixed glial-neuronal non-cancerous cells from Sprague-Dawley rats were also utilized to measure the effects of treatments on non-cancerous cells. Based on median inhibitory concentration values, the data belonged to non-cancerous cells (2486.71 mg/l) showed distinct towering compared to U87MG (80.93 mg/L) cells. The viability of non-cancerous and U87MG cells exposed to extract is decreased in a dose dependent manner. It was also showed that low concentrations of extract notably increased total antioxidant capacity on non-cancerous cells. In addition, various phenolic compounds in extract were detected through high-performance liquid chromatography. The recent results encourage that extract will be able to have therapeutic potential against glioblastoma.

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Section: Primary Mixed Glial-neuronal Cell Culturesmentioning

confidence: 99%

Inhibition of growth of U87MG human glioblastoma cells by Usnea longissima Ach.

Emsen

Özdemir

Engin

et al. 2019

An. Acad. Bras. Ciênc.

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“…The pharmaceutical effects of medicinal herbs include anti-inflammatory [14], antibiotic [15], and immunoregulatory activities [16]. In recent studies, extracts from several types of plants, such as lichens, Juniperus, and Angelica, were proven to be effective against brain tumor cell progression and angiogenesis [17][18][19]. More than half of the pharmaceutical herbal therapeutics are extracted from natural dietary plants, which have fewer side effects than other chemotherapeutic agents [20].…”

Section: Introductionmentioning

confidence: 99%

Juniperus Communis Extract Exerts Antitumor Effects in Human Glioblastomas Through Blood-Brain Barrier

Цай

Tsai

Chang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Herbal materials derived from Juniperus communis (JCo) possess anticancer activity. In this study, we evaluated the efficacy of a JCo berry extract in suppressing glioblastoma growth. Methods: The effects of JCo extract on the viability of normal and glioma cell lines was analyzed using a modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The synergistic therapeutic effect of JCo extract and temozolomide (TMZ) on glioma cells was examined by MTT analysis. Flow cytometry analysis, the terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) test, and western blotting were performed to identify the apoptotic pathway. To determine the in vivo efficacy of the JCo extract, rats were injected with 5 × 10⁴ rat glioma RG2 cells in the back skin and brain hemisphere and then received a subcutaneous injection in the back skin that contained either JCo extract or vehicle. Finally, blood and histologic examinations were performed to evaluate JCo toxicity. Results: The IC₅₀ values of JCo extract were 57–69 µg/mL and 49–67 µg/mL in the glioblastoma cell lines after 24 and 48 h, respectively. However, in non-tumor cell lines, the respective IC₅₀ values of JCo extract were 76–105 µg/mL and 77–108 µg/mL. The JCo extract had a stronger cytotoxicity and a larger range of IC₅₀ values in glioma than in normal cells as compared to those effects caused by temozolomide (TMZ). In addition, the results of flow cytometry analysis, TUNEL test, and western blotting revealed that the JCo extract induced glioma cell cycle arrest through intrinsic and extrinsic apoptotic pathways. In the in vivo studies, a significant reduction of tumor size in JCo-treated rats, as measured by animal MRI, demonstrated that the JCo extract effectively inhibited glioma cell growth and successfully penetrated the blood-brain barrier. The immunohistochemical (IHC) staining detected positive signals of PCNA, VEGFR-1, and VEGFR -2 in 44.49%, 5.88%, and 5.85% of JCo-treated glioma cells, respectively. However, positive signals of PCNA, VEGFR-1, and VEGFR-2 were detected in 73.08%, 9.67%, and 11.70% of vehicle-treated glioma cells, respectively. The IHC examination of PCNA and VEGFR-1 and -2 indicated that JCo extract significantly decreased the degree of neovascularization. However, no significant differences in serum levels of blood cell count and hepatic enzymes, renal function index, and the histologic appearance of vital organs were detected between the JCo and vehicle-treated rats. Conclusion: The JCo extract penetrated the blood-brain barrier and significantly induced glioma cell apoptosis by reducing neovascularization via suppression of the PI3K/AKT/mTOR pathway. Furthermore, JCo extract was less cytotoxic to non-neoplastic vital organs than TMZ.

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“…Especially after the 1990s, new usage areas of medicinal and aromatic plants increase the use of these plants day by day [Lange 2006]. Naturally occurring substances in many plant species are used in various treatments and show positive results [Emsen et al 2016[Emsen et al , 2017. This study presents antioxidant activities of water and methanol extracts of C. demersum propagated via tissue culture techniques.…”

Section: Resultsmentioning

confidence: 99%

EVALUATION OF ANTIOXIDANT ACTIVITY OF in vitro PROPAGATED MEDICINAL Ceratophyllum demersum L. EXTRACTS

Emsen

Doğan

2018

Acta Sci.Pol.Hortorum Cultus

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Ceratophyllum demersum L. is a medical plant of the family Ceratophyllaceae that has been traditionally used for the treatment of diseases such as ulcer, diarrhoea, wounds and fever since ancient times. This study presents antioxidant activities of water and methanol extracts of in vitro propagated C. demersum. Shoot tip explants of C. demersum for in vitro plant regeneration were cultured on liquid Murashige and Skoog (MS) medium containing 0.25-1.25 mg/L 6-Benzylaminopurine (BAP) and 0.10 mg/L Thidiazuron (TDZ) combinations for eight weeks. Maximum number of plantlets per explant (110.67) was obtained on MS medium supplemented with 0.75 mg/L BAP + 0.10 mg/L TDZ. In order to determine antioxidant activities of C. demersum, antioxidant contents (β-carotene, flavonoid, lycopene and total phenols), metal chelating and reducing power capacities of methanol and water extracts obtained from C. demersum were investigated. It was detected that water extract was more effective in all activities. Total phenols (76.55 μg/mg) in water extract were measured as the highest antioxidant component. Based on the water extracts data, as IC 50 value for chelating on ferrous ions was 9.24 mg/mL, EC 50 value for reducing power activity was 8.23 mg/mL. It was also determined that the metal chelating and reducing power activities of the both extracts are increased depending on the concentration. Therefore, all these data suggest that both tested extracts of in vitro propagated C. demersum would exert beneficial effects by virtue of their antioxidant properties and may be utilized as a promising source of therapeutics.

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In vitroantitumor activities of the lichen compounds olivetoric, physodic and psoromic acid in rat neuron and glioblastoma cells

Cited by 63 publications

References 58 publications

Inhibition of growth of U87MG human glioblastoma cells by Usnea longissima Ach.

Inhibition of growth of U87MG human glioblastoma cells by Usnea longissima Ach.

Juniperus Communis Extract Exerts Antitumor Effects in Human Glioblastomas Through Blood-Brain Barrier

EVALUATION OF ANTIOXIDANT ACTIVITY OF in vitro PROPAGATED MEDICINAL Ceratophyllum demersum L. EXTRACTS

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