The isolation, characterization endophytic bacteria on indole-3-acetic acid-producing and phosphate solubilizing from roots of local rice plant Kamba in Bada Valley, Central Sulawesi

Abstract

Abstract. Sudewi S, Ala A, Baharuddin, Farid M. 2020. The isolation, characterization endophytic bacteria on from roots of local rice plant Kamba in Central Sulawesi, Indonesia. Biodiversitas 21: 1614-1624. The local Kamba rice plants are cultivated by the Bada Valley people for generations by obtaining seeds from previous crops. This rice plant is able to survive even though it is cultivated with traditional cultivation techniques. The ability to survive is suspected because this local rice plant is associated with endophytic bacteria through a variety of mechanisms including being able to produce the IAA hormone and its ability to dissolve phosphate. This study aims to find endophytic bacteria that have the potential to produce IAA and phosphate solvents through morphological and physiological characterization (Gram reaction test with 3% KOH, catalase test, hypersensitivity reaction and hemolysis activity), screening of IAA-producing bacteria qualitative and quantitative, phosphate dissolution activity on Pikovskaya medium, and quantitative using a spectrophotometer. Endophytic bacteria isolates from Gintu give the highest yields in IAA production and phosphate solubility activity, namely RKGU11 and RKGU6, respectively at 4,905 mg L-1 and 10.984 mg L-1 so that it has the potential to be used as a candidate for biofertilizer agents in developing effective sustainable agriculture, respectively, efficient and environmentally friendly.

Microbial seed coating: An attractive tool for sustainable agriculture

“…Some research has demonstrated that PGPB can be effective in increasing the production of phytohormones (e.g., auxins, cytokinins, and gibberellins) [ 36 ]. The production of some phytohormones, such as ABA, by PGPB can be due to aquaporin upregulation under stress conditions [ 37 ].…”

Application of Organic Fertilizer Cow Dung and Biofertilizer in Shallots (Allium Acalonicum L.) in Lowland

“…The activity of Azospirillum and Azotobacter bacteria was to provide N elements, and some were able to provide P for plants and could produce growth hormones such as indole acetic acid (IAA). Azotobacter and Azospirillum bacteria will tether N from the air and convert it to NH 3 using the nitrogenase enzyme found in the bacterial cell wall, then NH 3 was converted into glutamine or alanine, so that it could be absorbed by plants in the form of NO 3 and NH 4 + (Goswami 2015;Sudewi et al 2020; Sutariati 2020). Phosphate solubilizing bacteria were bacteria that could dissolve P which was absorbed by the surface of iron and aluminum oxides as Fe-P and Al-P compounds (Novatriana 2020;Tuhuteru et al, 2017), so that P nutrients were released from metal compounds (Fe and Al).…”

Growth and yield response of shallot (Allium ascalonicum L. var. Tuktuk) from different source materials applied with liquid biofertilizers

“…The bacteria will tether N from the air and convert it to NH3 by using nitrogenase, then NH3 is converted to glutamine or alanine, so that it can be absorbed by plants in the form of NO3 and NH 4+ . N-fixing bacteria and phosphate solvents are effective, their population in the soil is only about 0.1-0.5% of the total microorganisms present (Goswami 2015;Sudewi et al 2020;Sutariati 2020).…”