The intraperitoneal inoculation of Lactobacillus casei in mice induces total protection against Trichinella spiralis infection at low challenge doses

Abstract

The following effects of Lactobacillus casei in NIH mice were evaluated: the establishment of Trichinella spiralis adult worms in the intestine (AWI), larvae per gram of muscle tissue (LPG), levels of IgG and IgA, and levels of IL-4 and IFN-γ. One hundred and eight mice were allocated at random into 18 groups of six mice each. Each mouse in treated or non-treated groups was inoculated intraperitoneally once a week during 6 weeks with L. casei or phosphate-buffered solution. Later each mouse was challenged either with 200, 50, or 25 T. spiralis infective larvae. When the infection dose was 200 T. spiralis infective larvae, the reductions in AWI were 78.6% at 4 days after infection (dai) and 76.7% at 10 dai; while the reduction of LPG was 80.9% with respect to control groups. When the infection dose was 50 or 25 T. spiralis infective larvae, the reductions of AWI were 100% both at 4 and 10 dai; while the reduction of LPG at 30 dai was also 100% with respect to control groups. The levels of IgG and IgA anti-T. spiralis and IL-4 were significantly higher (P < 0.01) at 4 and 10 dai in mice from groups treated with L. casei than in animals in control groups; while at 10 dai, the levels of IFN-γ were higher in control mice (P < 0.01) than in L. casei-treated animals. The results suggest that frequent treatment of mice with L. casei induces a total protection against infection with low doses of T. spiralis.

PROTECTIVE EFFECT OF THE PROBIOTIC Saccharomyces boulardii IN Toxocara canis INFECTION IS NOT DUE TO DIRECT ACTION ON THE LARVAE

“…The authors report that this probiotic induces a total protection against Trichinella spiralis infection at low doses16. Similar results were obtained in BALB/c mice with inhibited T. spiralis infections and invasion of the intestinal mucosa18.…”

Effect of probiotic acidophilus plus against infection with secondary hydatid disease in BALB /c mice

“…A suspension of Lactobacillus acidohilus, L. delbruckii revealed potential leverage against canine ancylostomiasis (16). Researchers observed that L. casei has anthelminthic 75-100% prophylactic effect, and Lactobacillus plantarum P164 (17) revealed 90% protection against T. spiralis, ensured that these strains are safe as protective and curative probiotics (17,23,35,36), A suspension for different probiotics revealed an increased worthy preservation 90% against Anchylostoma caninum infection in dogs (16), otherwise, Bifidobacterium animalis 04450B dose 9*10 2 CFU showed less protection 33% decrease of adult worms and 21% decrease of egg production Strongyloids venesuelensis infection in mice (37). First of all, we used orally administration with probiotic bacteria in experimental animals, but we didn´t see significant or obvious results that ensure the effect of bacteria as stimulative, when compared with the control group, so we used intraperitoneally injection because larval stages (hydatid cysts) are extraintestinal, so that the effect of intraperitoneal injection was very effectively obvious than oral administration.…”

Histopathology of murine toxoplasmosis under treatment with dialyzable leukocyte extract

“…NIH mice are laboratory rodents that best represent the genetic variability in a typical population, so they can simulate the heterogeneity of the human population and thus be used to evaluate the effect of DLEc under these conditions. In addition, these rodents have been used as an alternative murine model in pharmacological and immunological studies of some parasitic infections, including toxoplasmosis ( Martínez-Gómez et al 2009 , 2011 ).…”