The interplay between sulphur and selenium metabolism influences the intracellular redox balance in Saccharomyces cerevisiae

Mapelli, Valeria; Hillestrøm, Peter René; Patil, K. D.; Larsen, Erik Huusfeldt; Olsson, Lisbeth

doi:10.1111/j.1567-1364.2011.00757.x

Cited by 32 publications

(20 citation statements)

References 49 publications

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“…In conditions of sulfur deficiency, selenium uptake by S. cerevisiae cells led to an intracellular redox imbalance, linked to a disproportionate ration between the reduced form of glutathione and the oxidized one, a circumstance shown to be detrimental for cell viability [46]. …”

Section: Discussionmentioning

confidence: 99%

P. brasiliensis Virulence Is Affected by SconC, the Negative Regulator of Inorganic Sulfur Assimilation

et al. 2013

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Conidia/mycelium-to-yeast transition of Paracoccidioides brasiliensis is a critical step for the establishment of paracoccidioidomycosis, a systemic mycosis endemic in Latin America. Thus, knowledge of the factors that mediate this transition is of major importance for the design of intervention strategies. So far, the only known pre-requisites for the accomplishment of the morphological transition are the temperature shift to 37°C and the availability of organic sulfur compounds. In this study, we investigated the auxotrophic nature to organic sulfur of the yeast phase of Paracoccidioides , with special attention to P. brasiliensis species. For this, we addressed the role of SconCp, the negative regulator of the inorganic sulfur assimilation pathway, in the dimorphism and virulence of this pathogen. We show that down-regulation of SCONC allows initial steps of mycelium-to-yeast transition in the absence of organic sulfur compounds, contrarily to the wild-type fungus that cannot undergo mycelium-to-yeast transition under such conditions. However, SCONC down-regulated transformants were unable to sustain yeast growth using inorganic sulfur compounds only. Moreover, pulses with inorganic sulfur in SCONC down-regulated transformants triggered an increase of the inorganic sulfur metabolism, which culminated in a drastic reduction of the ATP and NADPH cellular levels and in higher oxidative stress. Importantly, the down-regulation of SCONC resulted in a decreased virulence of P. brasiliensis, as validated in an in vivo model of infection. Overall, our findings shed light on the inability of P. brasiliensis yeast to rely on inorganic sulfur compounds, correlating its metabolism with cellular energy and redox imbalances. Furthermore, the data herein presented reveal SconCp as a novel virulence determinant of P. brasiliensis.

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Section: Discussionmentioning

confidence: 99%

P. brasiliensis Virulence Is Affected by SconC, the Negative Regulator of Inorganic Sulfur Assimilation

et al. 2013

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“…Indeed, the Se-metabolite profile of Se-yeast is influenced by cultivation conditions (Rao et al, 2010). For example, it has been recently shown that Se toxicity and Se-metabolite profile in Se-yeast are significantly affected by the amount of sulphur provided during the fermentation (Mapelli et al, 2012). Secondly, analytical limitations to SeMet determination in Se-yeast further complicate this issue (Kubachka et al, 2017).…”

Section: Se-yeast: Advantages and Disadvantagesmentioning

confidence: 99%

Selenium in Poultry Nutrition: from Sodium Selenite to Organic Selenium Sources

et al. 2018

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Selenium (Se) is an essential element in poultry nutrition and its bio-efficacy depends on its chemical form. A growing body of research proves that organic forms of Se, mainly selenomethionine (SeMet), in poultry diets have a range of important advantages over traditional sodium selenite. In fact, the organic Se concept considers SeMet as a storage form of Se in the chicken body. As chickens are not able to synthesize SeMet, its provision through diet is a key strategy to fight commercially relevant stresses. Indeed, in stress conditions, when increased selenoprotein expression requires additional Se, while its provision via feed usually decreases due to a reduction in feed consumption, Se reserves in the body (mainly in the muscles) could help maintain an effective antioxidant defense and prevent detrimental consequences of stresses. The poultry industry is looking for the most effective sources of organic Se for commercial use. In this review, advantages and disadvantages of main organic Se sources for poultry (Se-yeast, SeMet, and OH-SeMet) are analyzed, and future directions for the development of new Se sources are identified.

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“…It proved the activation of processes associated with metabolism of this element. Several authors [ 29 , 30 ] suggest that the presence of selenium in the culture medium effected the changes in the antioxidant system of yeast cells, which resulted in an increased protein biosynthesis of selenium-dependent proteins.…”

Section: Resultsmentioning

confidence: 99%

Influence of Selenium Content in the Culture Medium on Protein Profile of Yeast CellsCandida utilisATCC 9950

Kieliszek

Błażejak

Bzducha-Wróbel

2015

Oxidative Medicine and Cellular Longevity

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Selenium is an essential trace element for human health and it has been recognized as a component of several selenoproteins with crucial biological functions. It has been identified as a component of active centers of many enzymes, as well as integral part of biologically active complexes. The aim of the study was to evaluate the protein content and amino acid profile of the protein of fodder yeast Candida utilis ATCC 9950 cultured in media control and experimental enriched selenium. Protein analysis was performed using SDS-PAGE method consisting of polyacrylamide gel electrophoresis in the presence of SDS. The highest contents of soluble protein (49,5 mg/g) were found in yeast cells after 24-hour culture conducted in control (YPD) medium. In the presence of selenium there were determined small amounts of protein content. With increasing time of yeast culture (to 72 hours) the control and experimental media were reported to reduce soluble protein content. In electropherogram proteins from control cultures was observed the presence of 10 protein fractions, but in all the experimental cultures (containing 20, 30, and 40 mg/L selenium) of 14 protein fractions. On the basis of the molecular weights of proteins, it can be concluded that they were among others: selenoprotein 15 kDa and selenoprotein 18 kDa.

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The interplay between sulphur and selenium metabolism influences the intracellular redox balance in Saccharomyces cerevisiae

Cited by 32 publications

References 49 publications

P. brasiliensis Virulence Is Affected by SconC, the Negative Regulator of Inorganic Sulfur Assimilation

P. brasiliensis Virulence Is Affected by SconC, the Negative Regulator of Inorganic Sulfur Assimilation

Selenium in Poultry Nutrition: from Sodium Selenite to Organic Selenium Sources

Influence of Selenium Content in the Culture Medium on Protein Profile of Yeast CellsCandida utilisATCC 9950

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