Influence of Selenium Content in the Culture Medium on Protein Profile of Yeast Cells<i>Candida utilis</i>ATCC 9950

Kieliszek, Marek; Błażejak, Stanisław; Bzducha-Wróbel, Anna

doi:10.1155/2015/659750

Cited by 31 publications

(24 citation statements)

References 25 publications

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“…To visualize protein bands, the gels were stained with Coomassie Brilliant Blue R-250. The electrophoretically separated proteins were documented with the GelDoc 2000 gels registration system (Bio-Rad, France) and analyzed using Quantity One computer program [21,22].…”

Section: Protein Extraction and Gel Electrophoresismentioning

confidence: 99%

Characterization of structure and protein of vitelline membranes of precocial (ring-necked pheasant, gray partridge) and superaltricial (cockatiel parrot, domestic pigeon) birds

2020

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Of all the known oviparous taxa, female birds lay the most diverse types of eggs that differ in terms of shape, shell pigmentation, and shell structure. The pigmentation of the shell, the weight of the egg, and the composition of the yolk correlate with environmental conditions and the needs of the developing embryos. In this study, we analyzed the structure and protein composition of the vitelline membrane (VM) of ring-necked pheasant, gray partridge, cockatiel parrot, and domestic pigeon eggs. We found that the VM structure is characteristic of each species and varies depending on whether the species is precocial (ring-necked pheasant and gray partridge) or superaltrical (cockatiel parrot and domestic pigeon). We hypothesize that a multilayer structure of VM is necessary to counteract the aging process of the egg. The multilayer structure of VM is only found in species with a large number of eggs in one clutch and is characterized by a long incubation period. An interesting discovery of this study is the three-layered VM of pheasant and partridge eggs. This shows that the formation of individual layers of VM in specific sections of the hen's reproductive system is not confirmed in other species. The number of protein fractions varied between 19 and 23, with a molecular weight ranging from 15 to 250 kDa, depending on the species. The number of proteins identified in the VM of the study birds' eggs is as follows: chicken-14, ring-necked pheasant-7, gray partridge-10, cockatiel parrot-6, and domestic pigeon-23. The highest number of species-specific proteins (21) was detected in the VM of domestic pigeon. This study is the first to present the structure and protein composition in the VM of ringnecked pheasant, gray partridge, cockatiel parrot, and domestic pigeon eggs. In addition, we analyzed the relationship between the hatching specification of birds and the structure of the VM.

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Section: Protein Extraction and Gel Electrophoresismentioning

confidence: 99%

Characterization of structure and protein of vitelline membranes of precocial (ring-necked pheasant, gray partridge) and superaltricial (cockatiel parrot, domestic pigeon) birds

2020

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“…Protein content in the supernatants was determined according to the Lowry method. Bovine albumin (Sigma-Aldrich, Poland) was used to prepare a standard curve [7].…”

Section: Determination Of Protein Contentmentioning

confidence: 99%

“…Selenium at appropriate concentrations exhibits antioxidant and regulatory properties [5] and is considered to be a potential therapeutic agent [6]. In addition, in humans, 25 genes encoding selenoproteins that are enzymes or structural proteins have been discovered, whereas in yeasts, 12 such genes have been identified, which indicates a new role of selenium in the metabolism of eukaryotic cells [7].…”

Section: Introductionmentioning

confidence: 99%

Effect of selenium on growth and antioxidative system of yeast cells

et al. 2019

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Selenium exhibits health-promoting properties in humans and animals. Therefore, the development of selenium-enriched dietary supplements has been growing worldwide. However, it may also exhibit toxicity at higher concentrations, causing increased oxidative stress. Different species of yeasts may exhibit different tolerances toward selenium. Therefore, in this study, we aimed to determine the effect of selenium on growth and on the antioxidative system in Candida utilis ATCC 9950 and Saccharomyces cerevisiae ATCC MYA-2200 yeast cells. The results of this study have demonstrated that high doses of selenium causes oxidative stress in yeasts, thereby increasing the process of lipid peroxidation. In addition, we obtained an increased level of GSSG from aqueous solutions of yeast biomass grown with selenium supplementation (40-60 mg/L). Increased levels of selenium in aqueous solutions resulted in an increase in the activity of antioxidant enzymes, including glutathione peroxidase and glutathione reductase. These results should encourage future research on the possibility of a thorough understanding of antioxidant system functioning in yeast cells.

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“…Therefore, it also has an enzymatic and stabilizing function. Selenium prevents vascular diseases, stimulates the immune system and exhibits antiproliferative activity [2][3][4]. Selenium plays a role in optimizing thyroid function [5].…”

Section: Introductionmentioning

confidence: 99%

“…Due to the ability of yeasts to permanently incorporate macronutrients and trace elements into the structure of their cells, they have become a source of not only protein but also of microelements for humans and animals. In addition, the body absorbs these elements more efficiently in organic form than in mineral preparations [2,11]. Yeasts also exhibit decreased toxicity and increased efficacy in the treatment of deficiency of this element.…”

Section: Introductionmentioning

confidence: 99%

Accumulation of Selenium in Candida utilis Growing in Media of Increasing Concentration of this Element

et al. 2020

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Selenium is considered an essential component of all living organisms. Studies on the enrichment of yeast cells with selenium, using the ability of cell biomass to bind this element, are being reported more and more. Yeast cultures were cultivated in YPD medium enriched with Na2SeO3 salts for 72 h at 28 °C on a shaker utilizing reciprocating motion. Selenium in cell biomass was determined with the use of ICP–MS. It was observed that the addition of selenium to the experimental medium (in the range of 4–100 mg/L) increased the content of this element in the yeast cell biomass. During the extension of cultivation time, the number of yeast cells and biomass yield exhibited a decreasing trend. Based on the obtained results, it was concluded that yeast cells exhibited the ability to accumulate selenium in both logarithmic and stationary growth phases. The dose of 20 and 30 mg/L of selenium in the culture medium meets the expectations in terms of both the content of selenium bound to yeast cells (1944 ± 110.8 μg/g dry weight) under 48-h cultivation. The obtained results confirmed that the Candida utilis ATCC 9950 strain exhibits the ability to bind selenium, which means that the biomass of these yeasts may be used as a natural source of selenium in the diet of humans and animals.

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Influence of Selenium Content in the Culture Medium on Protein Profile of Yeast CellsCandida utilisATCC 9950

Cited by 31 publications

References 25 publications

Characterization of structure and protein of vitelline membranes of precocial (ring-necked pheasant, gray partridge) and superaltricial (cockatiel parrot, domestic pigeon) birds

Characterization of structure and protein of vitelline membranes of precocial (ring-necked pheasant, gray partridge) and superaltricial (cockatiel parrot, domestic pigeon) birds

Effect of selenium on growth and antioxidative system of yeast cells

Accumulation of Selenium in Candida utilis Growing in Media of Increasing Concentration of this Element

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