Effect of selenium on growth and antioxidative system of yeast cells

Kieliszek, Marek; Błażejak, Stanisław; Bzducha-Wróbel, Anna; Kot, Anna M.

doi:10.1007/s11033-019-04630-z

Cited by 78 publications

(55 citation statements)

References 40 publications

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“…Yeast cells under different kinds of stress may generate abundant GSH, which is involved in the cell protection against the damage from O 2− . The high level of GSH could be a marker of an acute oxidative stress [48]. The data suggest that under optimal conditions, mannitol, the synthesis pathway of which is branched from the glycolysis at the glucose-6-phosphate stage, is of great importance for the development of Y. lipolytica [16].…”

Section: Cytosol Carbohydrate Profilementioning

confidence: 94%

Soluble Sugar and Lipid Readjustments in the Yarrowia lipolytica Yeast at Various Temperatures and pH

et al. 2019

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Microorganisms cope with a wide range of environmental challenges using different mechanisms. Their ability to prosper at extreme ambient pH and high temperatures has been well reported, but the adaptation mechanism often remains unrevealed. In this study, we addressed the dynamics of lipid and sugar profiles upon different cultivation conditions. The results showed that the cells grown at various pH and optimal temperature contained mannitol as the major cytosol sugar alcohol. The elevated temperature of 38 °C led to a two- to three-fold increase in total cytosol sugars with concurrent substitution of mannitol for trehalose. Lipid composition in the cells at optimal temperature changed insignificantly at any pH tested. The increase in the temperature caused some drop in the storage and membrane lipid levels, remarkable changes in their composition, and the degree of unsaturated fatty acids. It was shown that the fatty acid composition of some membrane phospholipids varied considerably at changing pH and temperature values. The data showed a pivotal role and flexibility of the sugar and lipid composition of Y. lipolytica W29 in adaptation to unfavorable environmental conditions.

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Section: Cytosol Carbohydrate Profilementioning

confidence: 94%

Soluble Sugar and Lipid Readjustments in the Yarrowia lipolytica Yeast at Various Temperatures and pH

et al. 2019

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“…It occurs in many proteins, changing their conformational structure and functional activity. The right dose of selenium also affects the occurrence of changes in the activity of antioxidant enzymes and glutathione [7].…”

Section: Effect Of Selenium On the Number Of Yeast Cellsmentioning

confidence: 99%

“…Selenium is an element found in many enzymes, especially those with antioxidant activity: glutathione peroxidase (GPx), iodothyronine deiodinase (DIO) and thioredoxin reductase (TRxR). These enzymes prevent the formation of hydrogen peroxide and the peroxidation of phospholipid cell membranes [6,7].…”

Section: Introductionmentioning

confidence: 99%

Accumulation of Selenium in Candida utilis Growing in Media of Increasing Concentration of this Element

et al. 2020

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Selenium is considered an essential component of all living organisms. Studies on the enrichment of yeast cells with selenium, using the ability of cell biomass to bind this element, are being reported more and more. Yeast cultures were cultivated in YPD medium enriched with Na2SeO3 salts for 72 h at 28 °C on a shaker utilizing reciprocating motion. Selenium in cell biomass was determined with the use of ICP–MS. It was observed that the addition of selenium to the experimental medium (in the range of 4–100 mg/L) increased the content of this element in the yeast cell biomass. During the extension of cultivation time, the number of yeast cells and biomass yield exhibited a decreasing trend. Based on the obtained results, it was concluded that yeast cells exhibited the ability to accumulate selenium in both logarithmic and stationary growth phases. The dose of 20 and 30 mg/L of selenium in the culture medium meets the expectations in terms of both the content of selenium bound to yeast cells (1944 ± 110.8 μg/g dry weight) under 48-h cultivation. The obtained results confirmed that the Candida utilis ATCC 9950 strain exhibits the ability to bind selenium, which means that the biomass of these yeasts may be used as a natural source of selenium in the diet of humans and animals.

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“…Glutathione is one of the most essential redox-scavenging compounds in yeast cells. The ratio of the two different forms, reduced (GSH) and oxidized (GSSG, glutathione disulfide) and the GSSG:GSH rate predicts the physiological and oxidative state of the cell, with healthy cells having lower GSSG:GSH values [49][50][51]. The biosynthesis of great amounts of glutathione correlates with the yeast oxidative stress resistance.…”

Section: Metabolic Pathway Involved In the Response To Ethanol Stressmentioning

confidence: 99%

Delta-Integration of Single Gene Shapes the Whole Metabolomic Short-Term Response to Ethanol of Recombinant Saccharomyces cerevisiae Strains

et al. 2020

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In yeast engineering, metabolic burden is often linked to the reprogramming of resources from regular cellular activities to guarantee recombinant protein(s) production. Therefore, growth parameters can be significantly influenced. Two recombinant strains, previously developed by the multiple δ-integration of a glucoamylase in the industrial Saccharomyces cerevisiae 27P, did not display any detectable metabolic burden. In this study, a Fourier Transform InfraRed Spectroscopy (FTIR)-based assay was employed to investigate the effect of δ-integration on yeast strains' tolerance to the increasing ethanol levels typical of the starch-to-ethanol industry. FTIR fingerprint, indeed, offers a holistic view of the metabolome and is a well-established method to assess the stress response of microorganisms. Cell viability and metabolomic fingerprints have been considered as parameters to detecting any physiological and/or metabolomic perturbations. Quite surprisingly, the three strains did not show any difference in cell viability but metabolomic profiles were significantly altered and different when the strains were incubated both with and without ethanol. A LC/MS untargeted workflow was applied to assess the metabolites and pathways mostly involved in these strain-specific ethanol responses, further confirming the FTIR fingerprinting of the parental and recombinant strains. These results indicated that the multiple δ-integration prompted huge metabolomic changes in response to short-term ethanol exposure, calling for deeper metabolomic and genomic insights to understand how and, to what extent, genetic engineering could affect the yeast metabolome.

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Effect of selenium on growth and antioxidative system of yeast cells

Cited by 78 publications

References 40 publications

Soluble Sugar and Lipid Readjustments in the Yarrowia lipolytica Yeast at Various Temperatures and pH

Soluble Sugar and Lipid Readjustments in the Yarrowia lipolytica Yeast at Various Temperatures and pH

Accumulation of Selenium in Candida utilis Growing in Media of Increasing Concentration of this Element

Delta-Integration of Single Gene Shapes the Whole Metabolomic Short-Term Response to Ethanol of Recombinant Saccharomyces cerevisiae Strains

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