The interaction of anti-DNA antibodies with DNA antigen: Evidence for hysteresis for high avidity binding

Pisetsky, David S.; Shaffer, Robert; Armstrong, Dustin D.; Spencer, Diane M

doi:10.1016/j.clim.2021.108848

Cited by 6 publications

(5 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In dissociation assays, the 500 and 1000 mM concentrations produced similar decreases; although, the effects dissociation were possibly less than effects on association. This difference may relate to the time course in which DNA binding occurs as observed previously [ 32 ]. The effects of increasing ionic strength are notable in view of past results suggesting that antibodies to Z-DNA in SLE result from charge–charge interactions [ 22 , 24 ].…”

Section: Resultsmentioning

confidence: 67%

The Binding Properties of Antibodies to Z-DNA in the Sera of Normal Healthy Subjects

Pisetsky,

Gedye,

David

et al. 2024

IJMS

Self Cite

View full text Add to dashboard Cite

Antibodies to DNA are a diverse set of antibodies that bind sites on DNA, a polymeric macromolecule that displays various conformations. In a previous study, we showed that sera of normal healthy subjects (NHS) contain IgG antibodies to Z-DNA, a left-handed helix with a zig-zig backbone. Recent studies have demonstrated the presence of Z-DNA in bacterial biofilms, suggesting a source of this conformation to induce responses. To characterize further antibodies to Z-DNA, we used an ELISA assay with brominated poly(dGdC) as a source of Z-DNA and determined the isotype of these antibodies and their binding properties. Results of these studies indicate that NHS sera contain IgM and IgA as well as IgG anti-Z-DNA antibodies. As shown by the effects of ionic strength in association and dissociation assays, the anti-Z-DNA antibodies bind primarily by electrostatic interactions; this type of binding differs from that of induced anti-Z-DNA antibodies from immunized animals which bind by non-ionic interactions. Furthermore, urea caused dissociation of NHS anti-Z-DNA at molar concentrations much lower than those for the induced antibodies. These studies also showed IgA anti-Z-DNA antibodies in fecal water. Together, these studies demonstrate that antibodies to Z-DNA occur commonly in normal immunity and may arise as a response to Z-DNA of bacterial origin.

show abstract

Section: Resultsmentioning

confidence: 67%

The Binding Properties of Antibodies to Z-DNA in the Sera of Normal Healthy Subjects

Pisetsky,

Gedye,

David

et al. 2024

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Firstly, these interactions can increase the sensitivity of biosensors, allowing for the detection and measurement of specific DNA sequences or chemicals even at low concentrations. 50 Secondly, strong interactions can improve the selectivity of biosensors, reducing the likelihood of false positives or false negatives by enhancing the ability to differentiate between different DNA sequences or targets. 51 Additionally, strong interactions can lead to faster response times in biosensors, enabling quick and accurate detection of biological analytes or modifications to DNA structure.…”

Section: Resultsmentioning

confidence: 99%

Multicomponent synthesis of pyrido[2,3-b]pyrazine derivatives: electrochemical DNA sensing, nonlinear optical properties and biological activity

Rashid,

Khalid,

Ashraf

et al. 2023

RSC Adv.

View full text Add to dashboard Cite

show abstract

“…According to our data, IgG of SLE patients efficiently hydrolyze ODN (AC) 5 in solution (Figure 8), which may be related to the formation of antibodies to such motifs and their efficient recognition. There is also evidence that recognition and high-affinity DNA binding by the antibody depend on monogamous bivalence, in which both Fab sites of the IgG molecule contact the same polynucleotide chain [20,56]. We used short ODNs (10 nucleotides) to exclude the influence of the second Fab site of an IgG molecule.…”

Section: Discussionmentioning

confidence: 99%

“…Available data indicate that anti-DNA antibodies recognize single-stranded (ssDNA), doublestranded (dsDNA), and specific forms of DNA (e.g., B-DNA and Z-DNA) [16,17]. Electrostatic interactions with the DNA backbone are thought to play an important role in DNA binding by antibodies [18][19][20]. The enrichment of positively charged amino acids, such as arginine, in the complementarity determining regions (CDRs) of anti-DNA antibodies, promote interactions with the negatively charged phosphate groups of the DNA backbone [19,21].…”

Section: Introductionmentioning

confidence: 99%

“…The enrichment of positively charged amino acids, such as arginine, in the complementarity determining regions (CDRs) of anti-DNA antibodies, promote interactions with the negatively charged phosphate groups of the DNA backbone [19,21]. However, non-electrostatic interactions may also occur [20]. Nucleotide sequence and secondary nucleic acid structure are also important for binding, as evidenced by data on the specificity of anti-DNA antibodies to both conserved and non-conserved sequences [18,22,23].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hydrolysis of Oligodeoxyribonucleotides on the Microarray Surface and in Solution by Catalytic Anti-DNA Antibodies in Systemic Lupus Erythematosus

Novikova,

Ermakov,

Kostina

et al. 2023

CIMB

View full text Add to dashboard Cite

Anti-DNA antibodies are known to be classical serological hallmarks of systemic lupus erythematosus (SLE). In addition to high-affinity antibodies, the autoantibody pool also contains natural catalytic anti-DNA antibodies that recognize and hydrolyze DNA. However, the specificity of such antibodies is uncertain. In addition, DNA binding to a surface such as the cell membrane, can also affect its recognition by antibodies. Here, we analyzed the hydrolysis of short oligodeoxyribonucleotides (ODNs) immobilized on the microarray surface and in solution by catalytic anti-DNA antibodies from SLE patients. It has been shown that IgG antibodies from SLE patients hydrolyze ODNs more effectively both in solution and on the surface, compared to IgG from healthy individuals. The data obtained indicate a more efficient hydrolysis of ODNs in solution than immobilized ODNs on the surface. In addition, differences in the specificity of recognition and hydrolysis of certain ODNs by anti-DNA antibodies were revealed, indicating the formation of autoantibodies to specific DNA motifs in SLE. The data obtained expand our understanding of the role of anti-DNA antibodies in SLE. Differences in the recognition and hydrolysis of surface-tethered and dissolved ODNs need to be considered in DNA microarray applications.

show abstract

The interaction of anti-DNA antibodies with DNA antigen: Evidence for hysteresis for high avidity binding

Cited by 6 publications

References 45 publications

The Binding Properties of Antibodies to Z-DNA in the Sera of Normal Healthy Subjects

The Binding Properties of Antibodies to Z-DNA in the Sera of Normal Healthy Subjects

Multicomponent synthesis of pyrido[2,3-b]pyrazine derivatives: electrochemical DNA sensing, nonlinear optical properties and biological activity

Hydrolysis of Oligodeoxyribonucleotides on the Microarray Surface and in Solution by Catalytic Anti-DNA Antibodies in Systemic Lupus Erythematosus

Contact Info

Product

Resources

About