The inhibitory effects of different diets on the protease activities of Argyrosomus regius (Pisces, Scianidae) larvae as a potential candidate species

Abstract

The aim of this research was to gather preliminary data about the potential inhibitory effects of commercial diets on the protease activities of meagre larvae using in vitro techniques. Four commercial diets (Gemma Micro 150 (100-200 µm), Caviar (200-300 µm), Caviar (300-500 µm) and Perla Larva Proactive 4.0 (300-500 µm)) were tested in this study. The differences in the protease activities of meagre larvae during the sampling period were statistically significant (p < .05). The highest and lowest protease activities of meagre larvae were 393.97 ± 7.9 U/mg protein (7 DAH) and 9.64 ± 1.25 U/ mg protein (20 DAH), respectively. The digestive proteases of meagre larvae showed the greatest sensitivity to protease inhibitors present in Gemma Micro 150 (100-200 µm). In conclusion, Caviar (200-300 µm), Caviar (300-500 µm) and Perla Larva Proactive 4.0 (300-500 µm) are moderately advisable as the commercial diets in the feeding protocol of meagre larvae from 15 to 32 DAH except for more than 50% inhibitions, but not Gemma Micro 150 (100-200 µm).

Seasonal variations of digestive enzymes in sea cucumbers (Holothuria tubulosa, G. 1788) under culture conditions

“…In this sense, it could be attributable to some biotical/abiotical factors such as physiological characters, temperature, climate, food content, and demands of species to biochemical compounds which are likely to affect the protease enzyme activity (Kreeger, 1993;Reid & Rauchert, 1976). Previous studies conducted with different fish types also reported the effect of salinity (Moutou, Panagiotaki, & Mamuris, 2004), illumination (Suzer, Saka, & Fırat, 2006), starvation (Zeng et al, 2012), temperature (Sun et al, 2015), and different foods (Diken, Demir, & Naz, 2018) on protease enzyme activity. It is commonly known that enzymatic activity in cultured animals is strongly effected by abiotical factors such as salinity, pH, illumination, and water parameters, especially, temperature.…”

The Inhibitory Effects and Positive Contributions of Live Foods on Protease Activities of Meagre, Argyrosomus regius (Asso 1801), Larvae in vitro Assay

“…In this study and the other studies related to the production of meagre (Argyrosomus regius), which is an important species for the Mediterranean Basin, it was determined that the larvae developed rapidly [27][28][29][30][31][32][33][34]. This can be considered an important advantage for the feeding and culturing of meagre larvae.…”

“…However, it is important to determine the changes in the ontogenetic and enzymatic activities of the larvae as well as larval development. In relation to this topic, there have also been studies dealing with the enzymatic changes of the meagre [31,[33][34][35][36]. On the other hand, there is a lack of studies and an inadequacy of practical applications to evaluate the optimum potential of live food sources in terms of changes in larval development and enzymatic activities.…”

The Inhibitory Effects and Positive Contributions of Live Foods on Protease Activities of Meagre, <em>Argyrosomus regius</em> (Asso 1801) Larvae <em>in vitro</em> Assay

“…There are studies dealing with the production of meagre, Argyrosomus regius an important species for the Mediterranean Basin [27][28][29][30][31][32][33][34]. It has been found that the larvae of meagre are showing rapid development in these studies and in the study carried out.…”

“…However, it is important to determine the changes in the ontogenetic and enzymatic activities of the larvae as well as larval development. In relation to this topic, there are also studies dealing with the enzymatic changes of the meagre [31,[33][34][35][36]. On the other hand, the lack of studies and the inadequacy of practical applications to determine the potential of evaluation of live food sources due to changes in larval development and enzymatic activities have been determined.…”