The influence of thyroid hormones on mitochondrial mechanisms of blood neutrophils’ apoptosis in case of experimental periodontitis

Abstract

L'influence des hormones thyroïdiennes sur les mécanismes mitochondriaux de l'apoptose des neutrophiles sanguins en cas de parodontite expérimentale L'objectif de l'étude. Cette étude vise à étudier le rôle des mitochondries dans l'apoptose des neutrophiles sanguins en cas de parodontite associée à une hyper et une hypothyroïdie chez le rat. Méthodes. Des études expérimentales ont été menées sur des rats blancs, non lignés et blancs, âgés d'environ 4 mois. L'analyse des échantillons de cellules pour déterminer les espèces d'oxygène réactives a été évaluée par la méthode de la cytométrie au laser en flux, en utilisant du diacétate de 2,7-dichlorodihydrofluorescéine. Le pourcentage de neutrophiles à faible potentiel mitochondrial transmembranaire et le pourcentage de neutrophiles apoptotiques ont été évalués par la méthode de la cytométrie au laser en flux, à l'aide de kits spécifiques.

The influence of chronic hyper-homocysteinemia on characteristics of peripheral neutrophils’ programmed death in lipo-polysaccharide-induced periodontitis

“…Neutrophils produce marked bactericidal effect; they neutralize pathogens by releasing free radicals (in so called 'respiratory burst'), and then absorbing them through phagocytosis [28]. Moreover, neutrophil cells contain an array of hydrolytic enzymes, including proteases, lipases, and deoxyribonucleases, in addition to compounds specifically targeting the bacterial cell wall, such as lysozyme and lactoferrin [29,30]. Additionally, a new antibacterial defense mechanism has been discovered in recent years: neutrophil extra-cellular traps [31].…”

The Qualitative and Quantitative Structure of Oral Microbiocenosis in Rats with Periodontitis in a Setting of Hyper- and Hypothyroidism

“…However, limited data are available regarding the relationship between thyroid hormone imbalance and periodontal health [4,5]. Periodontitis is an immune inflammatory response which arises from the interaction between the periodontal-pathogenic bacteria and the host, thus, dysbiosis play an important role in its pathogenesis [6,7]. In periodontitis, the severity of dysbiosis directly correlates with poor oral hygiene, higher periodontal indices and the severity of tooth decay [8].…”

A Comparative Study of Connective Tissue Metabolism Indices in Experimental Comorbidity-Free Periodontitis and Periodontitis Combined with Thyroid Dysfunction

“…Periodontal disease is a chronic bacterial infection characterized by persistent inflammation, connective tissue breakdown, and alveolar bone destruction [ 10 , 11 ]. The aggression of periodontopathogenic microflora is associated with the presence in the shell of bacteria of proteolytic enzymes and endotoxins.…”